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KR-20260064817-A - Extraction method of functional materials from eel by-products using supercritical carbon dioxide

KR20260064817AKR 20260064817 AKR20260064817 AKR 20260064817AKR-20260064817-A

Abstract

The present invention relates to a method for extracting functional substances derived from eel by-products using supercritical carbon dioxide, and more specifically, to a method for extracting functional substances using supercritical carbon dioxide by pulverizing eel heads. In addition, the present invention provides a method for preparing a supercritical extract containing a functional substance derived from eel by-products having antioxidant, anti-inflammatory, antibacterial, anti-dementia, and anticancer activities, which can be utilized in various fields such as the food, pharmaceutical, and cosmetics industries.

Inventors

  • 전병수
  • 박진석

Assignees

  • 국립부경대학교 산학협력단

Dates

Publication Date
20260508
Application Date
20241029

Claims (10)

  1. A step of manufacturing powder by drying and grinding eel by-products; A step of separating oil from the above-mentioned eel byproduct powder using supercritical carbon dioxide; and A method for extracting a functional substance derived from an eel byproduct, comprising the step of extracting the functional substance from the oil-separated eel byproduct powder using a co-solvent and supercritical carbon dioxide.
  2. In Article 1, The above eel by-product is, A method for extracting a functional substance derived from eel by-products, characterized by one or more selected from the head, internal organs, skin, muscles, or bones of the eel.
  3. In paragraph 1, The step of separating the above oil is, A method for extracting functional substances derived from eel by-products, characterized by performing the process using supercritical carbon dioxide as a solvent at a flow rate of 20 to 30 mL/min at a temperature of 50 to 60℃ and a pressure of 20 to 40 MPa.
  4. In paragraph 1, The step of extracting the above functional substance is, A method for extracting functional substances derived from eel by-products, characterized by adding ethanol as the above-mentioned co-solvent and performing the process using supercritical carbon dioxide as a solvent at a flow rate of 5 to 10 mL/min at a temperature of 30 to 50℃ and a pressure of 20 to 40 MPa.
  5. In paragraph 1, The above functional substance is, A method for extracting a functional substance derived from eel by-products, characterized by comprising one or more substances from the group consisting of lysophosphatidic acid (LPA), phosphatidic acid (PA), cardiolipin (CD), phosphatidylglycerol (PG), lysophosphatidylethanolamine (LPE), lysophosphatidylcholine (LPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), sphingomyelin (SM), and phosphatidylserine (PS).
  6. In paragraph 1, The above functional substance is, A method for extracting a functional substance derived from eel by-products, characterized by having antioxidant, anti-inflammatory, antibacterial, anti-dementia, and anticancer activities.
  7. A health functional food composition for anti-inflammatory and antioxidant purposes, comprising as an active ingredient a functional substance derived from eel by-products extracted by the extraction method according to claim 1.
  8. A cosmetic composition for anti-inflammatory and antioxidant purposes, comprising as an active ingredient a functional substance derived from eel by-products extracted by the extraction method according to claim 1.
  9. An antibacterial composition comprising, as an active ingredient, a functional substance derived from eel by-products extracted by the extraction method according to claim 1.
  10. A pharmaceutical composition for the prevention or treatment of Alzheimer's dementia and cancer, comprising as an active ingredient a functional substance derived from eel by-products extracted by the extraction method according to claim 1.

Description

Extraction method of functional materials from eel by-products using supercritical carbon dioxide The present invention relates to a method for extracting functional substances derived from eel by-products using supercritical carbon dioxide, and more specifically, to a method for extracting functional substances using supercritical carbon dioxide by pulverizing eel heads. Phospholipids (PLs) are amphiphilic compounds that have attracted significant attention in the food and pharmaceutical industries due to their special nutritional and emulsifying properties. Substances with low polarity are classified into phosphoglycerides (PGs) and sphingomyelins depending on the attachment of glycerol or sphingosyl frames; in the case of PGs, the first and second hydroxyl groups of glycerol form ester bonds with FA, while the third group is esterified with a phosphate group to one of the alcohols of different molecular weights, such as choline, ethanolamine, inositol, and serine. Thus, the major phospholipids (PLs) are phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS). The pharmacological significance of PL includes its application in the treatment of dyslipidemia, neurological disorders, and liver diseases, as well as its modification of the immune system by activating specific and non-specific defense systems. Furthermore, PL constitutes the structural blocks of biomolecules, supports cell and organ membranes, and plays a crucial role in cellular biochemistry and physiology; in the food, pharmaceutical, and cosmetic industries, PL exhibits physicochemical properties suitable for dispersion, stabilization, emulsification, and wetting. In particular, PC is widely used as a component of liposomes or as an emulsifier because it can form a flexible bilayer capable of creating spherical structures, or liposomes, in water; additionally, due to its natural origin, it is sometimes used as a biodegradable and biocompatible component. Commercially, PL is obtained from soybeans, egg yolks, or brain tissue. Soybeans are the most frequent and studied source of lecithin to date, but soybean lecithin is mainly rich in saturated fatty acids and low in unsaturated fatty acids, and does not contain some important PUFAs including EPA and DHA. Marine PL originates from marine resources and is characterized by the presence of significant amounts of ω-3 PUFAs. Appropriate extraction methods are required to ensure the proper application of such PL and maintain consistent quality. PL is mostly extracted using organic solvents such as diethyl ether, chloroform, and hexane, but some of these solvents pose a problem as they can have harmful effects on the environment and the human body. Recently, scientists and industry stakeholders have shown great interest in applying SC- CO2 fluid, a hydrophobic and eco-friendly medium, as an alternative to conventional organic solvents for separating target compounds from various solid matrices. SC- CO2 extraction is a promising and eco-friendly technology for extracting edible oils and fats because it is non-toxic, non-flammable, and the extraction process is performed in a sealed chamber, resulting in significantly less oxidation of the extracted oil and residue compared to conventional solvent extraction. Additionally, the low critical temperature of CO2 prevents the decomposition of thermally degradable components present in the matrix. Since PL is a protic substance, it is difficult to extract even when varying the conditions of SC- CO2 . Therefore, by adding a polar cosolvent (e.g., ethanol) to SC- CO2 , the interfacial tension and viscosity of SC- CO2 are reduced, and density, diffusivity, viscosity, and solubility are improved, thereby enhancing the mass transfer rate and enabling effective recovery. Conger myriaster is a widely consumed fish in Japan and Korea, and unlike ordinary fish, it is known for its high protein and PUFA content. In particular, its high levels of EPA and DHA help reduce cholesterol, making it highly effective in preventing adult diseases such as hypertension, diabetes, and obesity, and thus possessing significant value as a fishery resource. Based on the above background technology, the inventors have completed the present invention regarding a method for extracting functional substances derived from eel by-products using supercritical carbon dioxide. Figure 1 is the result of analyzing the phospholipid composition of eel head-derived lipids extracted by various methods according to one embodiment or comparative example of the present invention using 31 P NMR. Figure 2 is the result of analyzing the cytotoxicity and anticancer activity of eel head-derived phospholipids extracted according to one embodiment or comparative example of the present invention. Figure 3 is the result of analyzing the anti-inflammatory activity of eel head-derived phospholipids extracted according to one embodiment or comparative example o