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KR-20260064822-A - Chitosan-coated exosomes with improved stability, antioxidant and anti-inflammatory effects and their uses

KR20260064822AKR 20260064822 AKR20260064822 AKR 20260064822AKR-20260064822-A

Abstract

The present invention relates to chitosan-coated exosomes with improved stability and uses thereof. More specifically, the present invention relates to chitosan-coated exosomes with improved dispersion stability and storage stability and uses thereof, for example, in antioxidants, anti-inflammatory agents, pharmaceutical compositions, quasi-drug compositions, cosmetic compositions, or food compositions.

Inventors

  • 최원일
  • 오혜련
  • 손판모

Assignees

  • (주) 테라베스트

Dates

Publication Date
20260508
Application Date
20241029

Claims (15)

  1. Exosomes; and An exosome with improved stability comprising chitosan coated on the surface of the exosome.
  2. In paragraph 1, An exosome with improved stability, characterized in that the weight ratio of the exosome to chitosan is 1:0.1 to 5.
  3. In paragraph 1, Exosomes with improved stability, characterized in that the size of the chitosan-coated exosomes is 10 to 500 nm.
  4. An antioxidant comprising, as an active ingredient, an exosome with improved stability according to any one of paragraphs 1 to 3.
  5. An anti-inflammatory agent comprising, as an active ingredient, an exosome with improved water solubility according to any one of paragraphs 1 to 3.
  6. A pharmaceutical composition for the prevention or treatment of diseases caused by excessive production of reactive oxygen species, comprising as an active ingredient exosomes with improved water solubility according to any one of claims 1 to 3.
  7. In paragraph 6, A pharmaceutical composition characterized in that the disease caused by the excessive generation of the above-mentioned reactive oxygen species is stroke, Parkinson's disease, Alzheimer's disease, aging, heart disease, eye disease, ischemia, arteriosclerosis, skin disease, asthma, inflammation, arthritis, autoimmune disease, hyperlipidemia, liver disease, diabetes, cancer, chronic ulcer, burn, or wound.
  8. A quasi-drug composition for the prevention or treatment of diseases caused by excessive production of reactive oxygen species, comprising as an active ingredient exosomes with improved water solubility according to any one of claims 1 to 3.
  9. In paragraph 8, A quasi-drug composition characterized in that the above-mentioned quasi-drug composition is a disinfectant cleaner, shower foam, mouthwash, wet wipe, detergent soap, hand wash, humidifier filler, mask, ointment, or filter filler.
  10. A cosmetic composition for preventing or improving symptoms or diseases caused by excessive production of reactive oxygen species, comprising as an active ingredient exosomes with improved water solubility according to any one of claims 1 to 3.
  11. In Paragraph 10, A cosmetic composition characterized in that the symptoms or diseases caused by the above-mentioned active oxygen are skin aging, wrinkle formation, skin pigmentation, atopy, acne, psoriasis, or eczema.
  12. In Paragraph 10, A cosmetic composition characterized by the above cosmetic being manufactured in the form of an ampoule, cream, lotion, toner, essence, or pack.
  13. A topical skin composition for promoting wound healing or skin regeneration, comprising as an active ingredient an exosome with improved water solubility according to any one of claims 1 to 3.
  14. A food composition for the prevention or improvement of symptoms or diseases caused by reactive oxygen species, comprising as an active ingredient exosomes with improved water solubility according to any one of claims 1 to 3.
  15. In Paragraph 14, A food composition characterized in that the symptoms or diseases caused by the above-mentioned active oxygen are skin aging, wrinkle formation, skin pigmentation, atopy, acne, psoriasis, or eczema.

Description

Chitosan-coated exosomes with improved stability, antioxidant and anti-inflammatory effects and their uses The present invention relates to chitosan-coated exosomes with improved stability, antioxidant and anti-inflammatory effects, and uses thereof. More specifically, the present invention relates to chitosan-coated exosomes with improved stability, antioxidant and anti-inflammatory effects, and uses thereof, for example, in antioxidants, anti-inflammatory agents, pharmaceutical compositions, quasi-drug compositions, cosmetic compositions, or food compositions. Exosomes refer to small membrane-bound vesicles secreted by various cells and are defined as a type of extracellular vesicle (EV). All cells exchange information with other cells or the external environment and secrete extracellular vesicles for this purpose. Exosomes range in size from approximately 50 to 200 nm and contain physiologically active substances such as proteins, lipids, and nucleic acids. Exosomes exist in various cells, including mammals, bacteria, and plants, and can be utilized in diagnosis and treatment as they reflect the state of the cells of origin. As double-phospholipid membrane structures, exosomes facilitate intracellular penetration and perform various physiological and pathological functions, such as immune responses and signal transduction. Recently, research on the various efficacies of exosomes has been conducted, and studies on their skin benefits, such as antioxidant and anti-inflammatory effects, are also beginning. Due to their various advantages and activities, exosomes are materials that can be utilized in fields such as pharmaceuticals, cosmetics, and food. However, despite these diverse advantages and applications, their use is limited due to their structural characteristics as phospholipid bilayers, which result in low dispersibility and a tendency to aggregate. Furthermore, they are unstable at high temperatures and can easily break during the manufacturing process of cosmetic formulations; these properties can reduce the stability of exosomes within the formulation and cause precipitation. Therefore, to maintain continuous activity, it is necessary to improve the stability within the formulation by increasing the dispersibility of exosomes in aqueous solutions. Prior art 1. 10-2022-0019228 A (2022.02.16) Figure 1 shows (A) size, (B) dispersion value, and (C) surface charge of a chitosan-coated exosome (cExosome) according to Example 1. Figure 2 shows the results of the stability evaluation of exosomes (Exo) and cExosome (1:2) according to Example 2, including (A) the size and (B) the variance of exosomes (Exo) and cExosome (1:2) after storage in DIW for 4 weeks, and (C) the size and (D) the variance of exosomes (Exo) and cExosome (1:2) after storage in PBS for 7 days. Figure 3 shows the results of the cytotoxicity evaluation of exosomes (Exo) and cExosome (1:2) at different concentrations according to Example 3. Figure 4 shows the results of evaluating the reactive oxygen species scavenging ability of exosomes (Exo) and cExosome (1:2) according to Example 4 at different concentrations. Figure 5 shows the results of the anti-inflammatory evaluation of exosomes (Exo) and cExosome (1:2) according to Example 5 at different concentrations. Figure 6 shows the results of cell wound healing between the control group and cExosome (1:2) according to Example 6, (A) a picture of NIH 3T3 24 hours after cExosome (1:2) treatment and (B) the distance between cells. Hereinafter, chitosan-coated exosomes with enhanced stability, antioxidant and anti-inflammatory effects according to specific embodiments of the invention and their uses will be described in detail. However, this is presented as merely one example of the invention and does not limit the scope of the invention; it is obvious to those skilled in the art that various modifications to the embodiments are possible within the scope of the invention. Throughout this specification, unless otherwise specified, the terms "include" or "contain" refer to the inclusion of any component (or constituent) without any particular limitation and should not be interpreted as excluding the addition of other components (or constituents). As used in this specification, the term "antioxidant" refers to the action of inhibiting oxidation. Although the human body maintains a balance between prooxidants and antioxidants, various factors can cause this balance to become unbalanced and shift toward prooxidation. This leads to oxidative stress, which induces potential cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of such oxidative stress, are unstable and highly reactive, easily reacting with various biomaterials. They attack macromolecules within the body, causing irreversible damage to cells and tissues, or resulting in mutations, cytotoxicity, and carcinogenesis. Reactive nitrogen species (RNS), such as NO, HNO2 , and ONOO- , are generated