KR-20260064842-A - Extracts of potentilla chinensis with improved antioxidant activity and method of producing the same

Abstract

The present invention relates to a method for preparing a Weilingcai extract, and more specifically, to a method for preparing a Weilingcai extract with enhanced antioxidant activity depending on the extraction method and solvent conditions.

Inventors

• 임순호
• 곽유진

Assignees

• 동신대학교산학협력단

Dates

Publication Date

20260508

Application Date

20241029

Claims (12)

1. Potentilla chinensis extract with enhanced antioxidant activity, extracted from the whole plant using an aqueous ethanol solution as a solvent.
2. In Article 1, The above extraction is performed by reflux extraction or ultrasonic extraction, Weilingcai extract with enhanced antioxidant activity.
3. In Article 1, The concentration of the above ethanol aqueous solution is 40 to 60%, Weilingcai extract with enhanced antioxidant activity.
4. In Article 1, The above Weilingcai extract has a total polyphenol content of 23.64 to 33.24 mg TAE (tannic acid equivalent)/g, Weilingcai extract with enhanced antioxidant activity.
5. In Article 1, The above Weilingcai extract has a total flavonoid content of 7.83 to 8.93 mg CE (catechin equivalent)/g, Weilingcai extract with enhanced antioxidant activity.
6. In Article 1, The above Weilingcai extract is one with increased radical scavenging activity, Weilingcai extract with enhanced antioxidant activity.
7. In Article 1, The above Weilingcai extract is one with increased ferric-reducing antioxidant power (FRAP), Weilingcai extract with enhanced antioxidant activity.
8. Step of preparing Weilingcai powder by drying and grinding the whole Weilingcai plant; A step of obtaining an ethanol extract by adding an aqueous ethanol solution to the above Weilingcai powder and extracting it; A step of filtering the obtained ethanol extract through a filter; A method comprising the step of obtaining the supernatant after centrifuging the filtered ethanol extract. Method for preparing Weilingcai extract with enhanced antioxidant activity.
9. In Paragraph 8, The above extraction is performed by reflux extraction or ultrasonic extraction, Method for preparing Weilingcai extract with enhanced antioxidant activity.
10. In Paragraph 8, The concentration of the above ethanol aqueous solution is 40 to 60%, Method for preparing Weilingcai extract with enhanced antioxidant activity.
11. In Article 9, The above reflux extraction method is performed at 100℃ for 120 minutes, Method for preparing Weilingcai extract with enhanced antioxidant activity.
12. In Article 9, The above ultrasonic extraction method is performed at 40℃ at 60kHz for 120 minutes, Method for preparing Weilingcai extract with enhanced antioxidant activity.

Description

Extracts of potentilla chinensis with improved antioxidant activity and method of producing the same The present invention relates to a method for preparing a Weilingcai extract, and more specifically, to a method for preparing a Weilingcai extract with enhanced antioxidant activity depending on the extraction method and extraction solvent conditions. Humans have long strived to maintain health and youth, and numerous studies have been conducted to this end. However, contrary to these efforts, reactive oxygen species (ROS), produced inside and outside the human body by various causes such as respiration, dietary habits, environmental pollution, alcohol consumption, smoking, chemicals, stress, and ultraviolet rays, have adverse effects on humans. The abnormal operation of the redox system is the primary cause of these ROS. In particular, an increase in ROS induces oxidative stress. Because ROS is structurally very unstable, it reacts with cells to cause cell and tissue damage, resulting in various pathological abnormalities such as diabetes, cardiovascular disease, neurological disease, and even DNA damage that leads to cancer and accelerates aging. Representative ROS include singlet oxygen ( 1 O 2 ), superoxide anion radical (O 2 ·- ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH · ). Singlet oxygen is a key molecule in photoaging, generated in the skin due to photosensitivity reactions; along with hydroxyl radicals, it is the most reactive of all reactive oxygen species, diffuses through membranes, and primarily destroys antioxidant enzymes present in the body, thereby compromising the antioxidant defense system. Superoxide anion radicals can be scavenged by the enzyme SOD (superoxide dismutase), but they are sometimes converted into hydrogen peroxide during this process. Superoxide anion radicals react with hydrogen peroxide (Fenton reaction) to generate hydroxyl radicals when transition metals such as Fe(III) are reduced to produce Fe(II). For example, the amount of iron increases in areas exposed to ultraviolet rays, and it is known that sweat contains amounts of transition metals capable of rapidly triggering radical generation reactions. Although hydrogen peroxide is broken down into water and oxygen by the enzyme catalase, it has a relatively long lifespan compared to other ROS and easily passes through cell membranes. Thus, superoxide anion radicals and hydrogen peroxide do not directly contribute significantly to tissue damage, but they accelerate oxidation in the human body by generating hydroxyl radicals, which are highly reactive ROS. In order to defend against these ROS, endogenous antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) function to prevent DNA damage, protein modification, and lipid peroxidation by maintaining a balance with ROS; however, there are limitations to relying solely on these in vivo antioxidant enzymes. Therefore, research has been conducted on the development of synthetic and natural antioxidants capable of enhancing this in vivo antioxidant defense system or regulating ROS. However, synthetic antioxidants such as BHT and BHA, which were used to prevent and treat aging and various diseases, have recently been reported to have safety issues when consumed in excessive amounts. To overcome these challenges, research is currently underway to develop antioxidants derived from natural products such as various herbal medicines, fruits, and vegetables. Meanwhile, *Potentilla chinensis* is a perennial herb belonging to the Rosaceae family and is also known as *Ttakjikkot*. It grows to a height of 30–60 cm; its leaves are pinnately deeply lobed and willow-leaf shaped, and its yellow flowers bloom in clusters at the tips of branches. It thrives on mountain slopes, roadsides, near fields, forest grasslands, and open fields, particularly in sandy soil along streams. The whole plant is used to treat hemoptysis, diarrhea, and colitis, and is widely used to reduce inflammation in cases of the common cold, gastrointestinal diseases, rheumatoid arthritis, gout, bronchial asthma, and coughs. It is known that the whole plant and roots contain large amounts of flavonoids, as well as components such as saponin, kaempferol, cyanidin, catechin, ascorbic acid, tannin, quercetin, p-coumaric acid, and ellagic acid. Previous studies on Potentilla chinensis have reported various effects of extracts from different parts of the plant on skin cells, the isolation and identification of major components of Potentilla chinensis root extracts, the anti-inflammatory effects of Potentilla chinensis chloroform extracts, and the effects of Potentilla chinensis root extracts as cosmetic ingredients. However, research on the correlation between suitable extraction methods and antioxidant evaluations of Potentilla chinensis extracts at different ethanol (EtOH) concentrations, as well as cytotoxicity evaluations and ROS scavenging activity, is cur