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KR-20260065106-A - Composition comprising fermented hemp stem exosomes and uses thereof

KR20260065106AKR 20260065106 AKR20260065106 AKR 20260065106AKR-20260065106-A

Abstract

The present invention relates to a composition comprising fermented hemp stem exosomes and the use thereof. It has been confirmed that the fermented hemp stem exosomes of the present invention have wound healing and skin regeneration efficacy, skin whitening efficacy, and antibacterial activity, so the composition can be used for wound treatment, antibacterial and skin improvement purposes.

Inventors

  • 박순영
  • 김한솔

Assignees

  • 주식회사 네오켄바이오

Dates

Publication Date
20260508
Application Date
20241031

Claims (10)

  1. A cosmetic composition for improving skin condition comprising exosomes derived from fermented hemp stems.
  2. A composition according to claim 1, wherein the fermented hemp stem product is fermented by inoculating it with lactic acid bacteria.
  3. A composition according to claim 1, wherein the fermented product comprises a fermented filtrate or a fermented extract.
  4. A composition according to claim 1, wherein the average diameter of the exosomes is 50 nm to 500 nm.
  5. A composition according to claim 1, characterized in that the exosomes are included at a concentration of 1.0 x 10⁵ to 1.0 x 10¹⁵ particles/mL.
  6. A composition according to claim 1, wherein the improvement of the skin condition comprises one or more selected from the group consisting of wound healing, skin regeneration, whitening, and skin antibacterial activity.
  7. A topical skin composition for improving skin condition, comprising exosomes derived from fermented cannabis stems.
  8. A pharmaceutical composition for wound healing or skin regeneration comprising exosomes derived from fermented cannabis stems.
  9. An antimicrobial composition comprising exosomes derived from fermented cannabis stems.
  10. The composition of claim 9, wherein the composition has antibacterial activity against Escherichia coli .

Description

Composition comprising fermented hemp stem exosomes and uses thereof The present invention relates to a composition comprising hemp stem fermented exosomes and the use thereof. Cannabis sativa L. is an annual plant of the genus Cannabis of the family Cannabaceae that has been widely cultivated in tropical and temperate regions, centered in Central Asia, for 12,000 years. It is a collective term for Cannabis chemovars containing various types of cannabinoid compounds known as medicinal and pharmaceutical components, as well as Cannabis sativa subspecies sativa, Cannabis sativa subspecies indica, Cannabis sativa subspecies ruderalis , including its variants var. indica and var. kafiristanica , and plants resulting from genetic crosses, self-crosses, or their hybridization. In traditional medical texts from China and Korea, hemp seeds with the hulls removed, known as *Majain* or *Hwamain*, have been used for constipation, diabetes, pain disorders, menstrual irregularities, skin diseases, and dysentery. Additionally, hemp leaves (*Macha*, *Mayeop*) have been used as an anthelmintic, hair protectant, for asthma, for pain relief, anesthesia, and as a diuretic. Furthermore, records indicate that hemp roots were used for treating difficult childbirth and resolving blood stasis, hemp bark for bruises and open wounds, hemp flowers for paralysis and itching, and hemp powder was used for difficult childbirth, constipation, gout, phlegm, and insomnia, with each part of the hemp plant being used according to the specific condition. Meanwhile, an exosome refers to a nano-sized extracellular vesicle that is secreted from inside a cell and released into the extracellular space. These exosomes contain substances similar to those found inside the parent cell, such as proteins, lipids, metabolites, and nucleic acids, and act as messengers for these substances between cells. Recently, technologies utilizing the function of exosomes to deliver substances between cells, such as drugs or vaccines to target organs, have been developed; however, research on the application of exosomes in the field of cosmetic dermatology remains somewhat lacking. Against this backdrop, the present invention was completed by confirming the significantly superior skin improvement efficacy, such as wound healing and skin regeneration, of exosomes derived from fermented cannabis stems. Figure 1 is a diagram showing the size of exosomes derived from fermented cannabis stems. Figure 2 is a diagram showing the results of confirming the cytotoxicity of exosomes derived from fermented cannabis stems. Figure 3 is a diagram showing the results of evaluating the wound healing efficacy of exosomes derived from fermented cannabis stems. Figure 4 is a diagram showing the results of evaluating the skin whitening efficacy of exosomes derived from fermented hemp stems. Figure 5 is a diagram showing the results of evaluating the antibacterial activity of exosomes derived from fermented cannabis stems against E. coli. The following examples will be explained in more detail. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples. Example 1: Preparation of Hemp Stem Fermentation Exosomes The following experiment was performed to produce and obtain exosomes derived from fermented cannabis stems (cannabis stem fermentation exosomes). Specifically, fermentation microorganisms ( Lactobacillus acidophilus ) were inoculated (OD 0.1) into 300 ml of culture medium and cultured at 30°C for 48 hours. Subsequently, 20 g of hemp stems were added to 200 ml of the culture medium and fermented at 30°C for 72 hours. Afterward, the obtained fermented product was squeezed using a press with sterile gauze to obtain only the fermentation culture medium. The fermentation culture medium was centrifuged at 3,000 rpm for 20 minutes to collect the supernatant, and the obtained supernatant was centrifuged at 10,000 rpm for 2 hours to collect only the supernatant, which was then vacuum filtered using a 0.8 μm membrane filter and a 0.2 μm membrane filter. PEG at a concentration of 10% w/v was added to the solution filtered from the fermentation medium to dissolve it completely, and the mixture was refrigerated for 20 hours. Afterwards, centrifugation was performed at 10,000 rpm for 1 hour, the supernatant was removed, and the precipitate was recovered with PBS. Next, NTA analysis was performed to determine the size and concentration of the cannabis stem fermentation exosomes produced through the above process. The analysis results confirmed that the average size of the cannabis stem fermentation exosomes was 149.2 nm and the concentration was 4.6 x 10¹² Particles/mL (Fig. 1). Example 2: Evaluation of Cytotoxicity of Hemp Stem Fermented Exosomes To evaluate the cytotoxicity of fermented cannabis stem exosomes, the following experiments were performed. Specifically, HaCaT cells (keratinocytes) were placed in a 100Φ dish with