KR-20260065560-A - Composition comprising exosomes loaded with cannabinoid and uses thereof

Abstract

The present invention relates to a composition comprising exosomes containing cannabinoids as an active ingredient and the use thereof. It has been confirmed that the exosomes containing cannabinoids of the present invention have wound healing and skin regeneration efficacy and anti-inflammatory activity, and thus the composition can be used for skin improvement purposes such as wound healing and anti-inflammation.

Inventors

• 김정국
• 송중호
• 박순영
• 박지수

Assignees

• 주식회사 네오켄바이오

Dates

Publication Date

20260508

Application Date

20251030

Priority Date

20241031

Claims (12)

1. A cosmetic composition for improving skin condition, comprising exosomes containing cannabinoids as an active ingredient.
2. A composition according to claim 1, wherein the exosomes are derived from plants or microorganisms.
3. A composition according to claim 2, wherein the plant comprises hemp.
4. A composition according to claim 1, wherein the exosomes are derived from a hemp stem or a fermented hemp stem.
5. A composition according to claim 1, wherein the cannabinoid comprises one or more selected from the group consisting of cannabidiol (CBD), cannabichromene, cannabinol, cannabigerol, tetrahydrocannabinol, and derivatives thereof.
6. A composition according to claim 1, wherein the cannabinoid is loaded into an exosome.
7. A composition according to claim 1, wherein the average diameter of the exosomes is 50 nm to 500 nm.
8. A composition according to claim 1, characterized in that the exosomes are included at a concentration of 1.0 x 10⁵ to 1.0 x 10¹⁵ particles/mL.
9. A composition according to claim 1, wherein the improvement of the skin condition comprises one or more selected from the group consisting of wound healing, skin regeneration, and anti-inflammatory.
10. A topical skin composition for improving skin condition, comprising exosomes containing cannabinoids as an active ingredient.
11. A pharmaceutical composition for wound healing or skin regeneration comprising exosomes containing cannabinoids as an active ingredient.
12. An anti-inflammatory composition comprising exosomes containing cannabinoids as an active ingredient.

Description

Composition comprising exosomes loaded with cannabinoid and uses thereof The present invention relates to a composition comprising exosomes containing cannabinoids as an active ingredient and the use thereof. Cannabis sativa L. is an annual plant of the genus Cannabis of the family Cannabaceae that has been widely cultivated in tropical and temperate regions, centered in Central Asia, for 12,000 years. It is a collective term for Cannabis chemovars containing various types of cannabinoid compounds known as medicinal and pharmaceutical components, as well as Cannabis sativa subspecies sativa, Cannabis sativa subspecies indica, Cannabis sativa subspecies ruderalis , including its variants var. indica and var. kafiristanica , and plants resulting from genetic crosses, self-crosses, or their hybridization. Cannabis contains about 400 compounds, most of which are cannabinoids, terpenes, and phenolic compounds. Among these, cannabinoids, which are important natural components for medicine and pharmaceuticals, number about 90, and many of these components are found only in cannabis. Among the cannabinoids of cannabis, the psychoactive component is 9-tetrahydrocannabinol (9-THC), and cannabidiol (CBD) is a non-psychoactive component known to exhibit physiological effects through various receptors in the human body, including adrenaline receptors and cannabinoid receptors. Meanwhile, an exosome refers to a nano-sized extracellular vesicle that is secreted from inside a cell and released into the extracellular space. These exosomes contain substances similar to those found inside the parent cell, such as proteins, lipids, metabolites, and nucleic acids, and act as messengers for these substances between cells. Recently, technologies utilizing the function of exosomes to deliver substances between cells, such as drugs or vaccines to target organs, have been developed; however, research on the application of exosomes in the field of cosmetic dermatology remains somewhat lacking. Against this backdrop, the present invention was completed by confirming the significantly superior skin improvement efficacy, such as wound healing and skin regeneration, of exosomes derived from cannabis stems loaded with cannabinoids derived from cannabis. Figure 1 is a diagram showing the size of hemp stem-derived exosomes loaded with cannabidiol. Figure 2 is a diagram showing the results of the cytotoxicity evaluation of hemp stem-derived exosomes loaded with cannabidiol. Figure 3 is a diagram showing the results of evaluating the wound healing efficacy of hemp stem-derived exosomes loaded with cannabidiol. Figure 4 is a diagram showing the results of evaluating the anti-inflammatory activity of hemp stem-derived exoskeletons loaded with cannabidiol. Figure 5 is a diagram showing the size of exosomes derived from cannabis stems and exosomes derived from cannabis stem fermentation products. Figure 6 is a diagram showing the results of analyzing the loading efficiency of exosomes loaded using ultrasonic treatment. Figure 7 is a diagram showing the results of analyzing the loading efficiency of cannabinoids loaded into exosomes derived from cannabis stems. Figure 8 is a diagram showing the results of analyzing the loading efficiency of cannabinoids loaded into exosomes derived from fermented cannabis stems. Figure 9 is a diagram showing the results of the cytotoxicity evaluation of exosomes derived from fermented cannabis stems loaded with cannabidiol. Figure 10 is a diagram showing the results of evaluating the wound healing efficacy of exosomes derived from fermented hemp stems loaded with cannabidiol. The following examples will be explained in more detail. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples. Example 1: Preparation of cannabidiol-loaded exosomes and evaluation of their activity 1.1: Manufacture of cannabidiol-loaded exosomes The following experiment was performed to prepare and obtain plant-derived exosomes loaded with cannabidiol. First, to obtain plant-derived exosomes, the target plant (specifically, cannabis stems) was washed and dried for 1 to 2 hours, then mixed with PBS buffer (pH 7.4) in a blender and subjected to high-speed grinding. The obtained ground material was pressed using a press with sterile gauze to collect only the ground liquid. The obtained ground liquid was centrifuged at 3,000 rpm for 20 minutes, and the supernatant was collected. Subsequently, the supernatant was collected by centrifuging at 8,000 rpm for 2.5 hours, and then filtered under reduced pressure using a 0.8 μm membrane filter and a 0.2 μm membrane filter. PEG at 10% w/v was added to the filtered solution and stirred to completely dissolve it, after which the solution was refrigerated for 20 hours. The refrigerated solution was centrifuged at 10,000 rpm for 1 hour, the supernatant was discarded, and the precipitate was recovered with PBS. Next, to load cannabid