Search

KR-20260065675-A - Composition for antimicrobial activity and skin whitening containing a mixture of Hydroxycurvularin diastereomers

KR20260065675AKR 20260065675 AKR20260065675 AKR 20260065675AKR-20260065675-A

Abstract

The present invention aims to provide a cosmetic composition for skin whitening comprising one or more selected from the group consisting of: a culture medium of Curvularia intermedia CNUFC 23CHB15 (accession number: KCTC16013BP); and a culture filtrate of said strain.

Inventors

  • 이향범

Assignees

  • 전남대학교산학협력단

Dates

Publication Date
20260511
Application Date
20241101

Claims (9)

  1. Culture of Curvularia intermedia CNUFC 23CHB15 (accession number: KCTC16013BP); and A cosmetic composition for skin whitening comprising one or more selected from the group consisting of the culture filtrate of the above strain.
  2. In paragraph 1, The above skin whitening cosmetic composition comprises a hydroxycurvurarin mixture as an active ingredient.
  3. In paragraph 2, A cosmetic composition for skin whitening, wherein the above hydroxycurburarin mixture comprises a compound of the following chemical formula 1 and a compound of the following chemical formula 2. [Chemical Formula 1] [Chemical Formula 2]
  4. In paragraph 2, The above cosmetic composition is a skin whitening cosmetic composition comprising a hydroxycurburarin mixture at a concentration of 1 to 50 μM.
  5. In paragraph 1, The above cosmetic composition is a skin whitening cosmetic composition prepared in a formulation selected from the group consisting of cream, foam, nourishing lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, soap, bath additive, sun cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, surfactant-containing cleansing, oil, patch, and spray.
  6. Step of preparing a culture medium of Curvularia intermedia CNUFC 23CHB15 (Accession No.: KCTC16013BP); A step of preparing an extract by adding an organic solvent to the above culture medium; and A method for preparing a mixture of hydroxycurvurarin diastereomers, comprising the step of separating a hydroxycurvurarin mixture by performing medium pressure liquid chromatography (MPLC) on the above extract.
  7. In paragraph 6, A method for preparing a hydroxycurvurarin diastereomer mixture, wherein the above hydroxycurvurarin mixture comprises a compound of Formula 1 and a compound of Formula 2. [Chemical Formula 1] [Chemical Formula 2]
  8. In paragraph 6, A method for preparing a hydroxycurvurarin diastereomer mixture, wherein the above culture medium is obtained by culturing the above-mentioned Curvuraria intermedia strain in an MEA medium at 20 to 40°C for 1 to 5 weeks.
  9. In paragraph 6, A method for preparing a mixture of hydroxycurburarin diastereomers, wherein the above-mentioned medium-pressure liquid chromatography (MPLC) is performed while applying a concentration gradient with an aqueous methanol solution.

Description

Composition for antimicrobial activity and skin whitening containing a mixture of hydroxycurvularin diastereomers The present invention relates to a cosmetic composition for skin whitening containing a mixture of partial stereoisomers of hydroxycurvurarin isolated from the fungus Curvuraria intermedia as an active ingredient. Fungi are widely utilized across all industrial sectors, including agriculture, medicine, pharmacy, food, chemistry, materials, biological restoration, and the environment. In particular, they are among the useful microorganisms that serve as raw materials for approximately 3,000 types of antibiotics in the pharmaceutical market. Additionally, there are about 70 types of microorganisms recognized by the Ministry of Food and Drug Safety as safe for use in food, and among them, a total of 3,179 papers related to fungal-derived antioxidants and anti-inflammatory agents have been published over the past five years. Korea accounts for the largest share (63%) of patents related to antioxidants and anti-inflammatory agents using fungi , and annual patent applications are also on the rise. However, research on developing other taxonomic groups as biomaterials, excluding Ascomycetes and Basidiomycetes , is very rare. Recently, consumer demand for cosmetics containing natural and organic ingredients has been increasing, and fungi belonging to the Ascomycota and Basidiomycota have been utilized as potential ingredients in the cosmetics industry, including skin whitening agents, moisturizers, anti-aging agents, shampoos, and various other products. Rhizopus arrhizus and R. oryzae , belonging to the Zygomycetes, are used in the production of lactic acid and glycolic acid, which are used as skin whitening agents. However, since more than 90% of raw materials are imported, the development of new cosmetic raw materials is necessary for domestic cosmetic companies to compete on equal footing with foreign companies. As the demand for new functional cosmetic ingredients increases, various materials are being explored, and among them, materials derived from various mushrooms and fungi are being tested. To date, skin whitening effects from extracts derived from natural products, such as mushroom extracts, have been known, but there have been no reports of skin whitening functional cosmetics derived from isolated and purified single compounds from fungal strains. Figure 1 shows the phylogenetic tree of the Curvuraria intermedia CNUFC 23CHB15 strain. Figure 2 shows the process (A) of extracting compound 1 (hydroxycurvularin mixture), compound 2 (α,β-Dehydroxycurvulain), and compound 3 (Curvularin) from the culture medium of a Curvularia intermedia strain, and the MPLC (Medium Pressure Liquid Chromatography) results (B). Figure 3 shows the results of analyzing compound 1 (hydroxycurvularin mixture) via 1H -NMR. Figure 4 shows the results of analyzing compound 2 (α,β-Dehyrocurvulain) via 1H -NMR. Figure 5 shows the results of analyzing compound 1 (Curvularin) via 1H -NMR. Figure 6 shows the antibacterial activity of compound 1 (11-hydroxycurvularin mixture) and compound 2 (α,β-Dehydroxycurvularin). Figure 7 shows the cell viability of B16F10 cells treated with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin as a positive control. Figure 8 shows the cell proliferation rates of B16F10 cells treated with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin, a positive control. Figure 9 shows the results of the reduction in the accumulation of melanin produced by α-MSH following treatment with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin as a positive control (left), and the cell viability of B16F10 cells following simultaneous treatment with α-MSH and each substance (right). Figure 10 shows the reduction in melanin content produced by α-MSH following treatment with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin as a positive control. Figure 11 shows the reduction in the activity of intracellular tyrosinase activated by α-MSH following treatment with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin as a positive control. Figure 12 shows the reduction in gene expression of tyrosinase, Tyrp1, and Tyrp2 over time following treatment with a hydroxycurvularin mixture, two isomers included in the mixture, and arbutin, a positive control. The present invention will be described in detail below. Unless otherwise defined, terms used in this specification should be interpreted as generally understood by those skilled in the art. The drawings and embodiments of this specification are intended to enable those skilled in the art to easily understand and practice the present invention; therefore, details that may obscure the essence of the invention may be omitted from the drawings and embodiments, and the present invention is not limited to the drawings and embodiments. The singula