KR-20260065767-A - OBSERVATION METHOD AND APPARATUS USING OPTICAL DEVICE

KR20260065767AKR 20260065767 AKR20260065767 AKR 20260065767AKR-20260065767-A

Abstract

One embodiment of the present disclosure provides an observation device comprising: a first polarizing unit that polarizes light emitted from a light source unit; a light splitting unit that splits light passing through the first polarizing unit into a first reflected light and a first transmitted light; an objective lens that collects light containing information of an object based on the first reflected light; an image sensor that detects input light based on the light collected by the objective lens; and a second polarizing unit that blocks the first transmitted light so that the first transmitted light is not detected by the image sensor.

Inventors

박영호
안성은
김봉우
조태연

Assignees

주식회사 큐리오시스

Dates

Publication Date: 20260511
Application Date: 20260415
Priority Date: 20240926

Claims (15)

As an observation device, A first polarizing unit that polarizes light emitted from a light source unit; A light splitting unit that splits the light passing through the first polarizing unit into a first reflected light and a first transmitted light; An objective lens that collects light containing information of an object based on the first reflected light above; An image sensor that detects input light based on light collected by the above objective lens; and A second polarizing unit that blocks the first transmitted light so that the first transmitted light is not detected by the image sensor. An observation device including
In paragraph 1, the light detected by the image sensor is, The first reflected light is scattered from above the object and the scattered light is transmitted light that passes through the object; or The scattered light of the first reflected light scattered from the object including, Observation device.
In paragraph 2, The light scattered from above the object by the first reflected light is, An observation device comprising light scattered by at least one of the top plate of a container or the surface of a media.
In paragraph 3, the light collected by the objective lens is, It includes light reflected by at least one of the above object, the above container, or the above media surface, and An observation device in which the above reflected light is blocked by the second polarizing part when it is light that has not undergone a scattering process.
In paragraph 1, the observation device is, An observation device further comprising the light source unit provided inside the observation device.
In claim 1, the polarization angle of the first polarization part is, An observation device characterized by forming a 90-degree angle with the polarization angle of the second polarization part.
In claim 1, the second polarizing unit is, Polarizing the light collected by the above objective lens, The above image sensor is, An observation device that acquires light polarized by the second polarizing unit.
In paragraph 1, the object is contained in a single-layer container or a multi-layer container, Observation device.
In claim 8, the multilayer vessel is CellSTACK™, HYPERStack™, Cell Factory™, Nunc™ EasyFill™ Cell Factory, Falcon® Cell Culture Multi-Layer Flask, STACKMAX™, iCELLis™ Nano and iCELLis™ 500+, TripleFlask™ system, or HYPERFlask® Observation device.
In paragraph 1, the object is contained in a container, and The above container is composed of a bottom plate and a top plate, or is composed only of a bottom plate. Observation device.
In paragraph 1, the object is contained in a container, and The above container comprises an optically transparent material, Observation device.
In paragraph 1, the observation device is, An observation device that is a live cell imaging system.
Regarding the method of observation, A step of emitting light by a light source; A step of polarizing the light emitted by the light source unit by the first polarizing unit; A step of dividing the light that has passed through the first polarizing unit into a first reflected light and a first transmitted light by means of a light splitting unit; A step of blocking the first transmitted light by the second polarizing unit so that the first transmitted light is not detected by the image sensor; A step of collecting light containing information of an object by means of an objective lens based on the first reflected light; An observation method comprising the step of detecting light input to the image sensor based on light collected by the objective lens.
In paragraph 13, the light detected by the image sensor is, The first reflected light is scattered from above the object and the scattered light is transmitted light that passes through the object; or The scattered light of the first reflected light scattered from the object An observation method that includes
As a method of observation, A step in which at least a portion of the light emitted from a light source is scattered on an object or above the object; and An observation method comprising the step of detecting scattered light scattered from the object, or transmitted light scattered from above the object and then transmitted through the object.

Description

Observation method and apparatus using an optical device The present disclosure relates to an observation method and apparatus using an optical device. More specifically, the present disclosure relates to a method and apparatus for observing an object using a polarizing element that polarizes light. Optical devices, such as microscopes, are used as important tools in various disciplines including biology, chemistry, and materials science because they allow for the magnification and observation of minute objects. In particular, optical systems equipped with in-line illumination allow light to be incident perpendicularly on the surface, reducing scattering and facilitating observation of objects without distortion caused by reflection. Furthermore, since the illumination and observation paths align, a separate mechanism for adjusting the illumination angle is not required, thereby simplifying the design of the optical device. However, in-line illumination is primarily used for observing macroscopic objects such as metals and other objects, and in-line illumination systems are not utilized in microscopes for biological applications, such as the detailed structures of cells, tissues, proteins, and other biomaterials. This is because transmitting illumination is required to observe transparent objects like cells, necessitating appropriate control of light intensity; however, the light emitted by the light source from in-line illumination is too strong to see the light emitted by microscopic and transparent cells. The present disclosure aims to solve the problem that in-line illumination cannot be used in an observation device for observing such cells, etc. FIG. 1 is a drawing showing an optical system using an in-line illumination method according to one embodiment of the present disclosure. FIG. 2a is a diagram showing the process of light emitted from a light source unit according to one embodiment of the present disclosure reaching an object. FIG. 2b is a diagram showing the process of light passing through an object of observation according to one embodiment of the present disclosure reaching an image acquisition unit. FIG. 3 is a drawing showing an image obtained by observing a macroscopic object using an observation device according to one embodiment of the present disclosure. FIGS. 4a and 4b are drawings showing images obtained by observing a microscopic object using an observation device according to one embodiment of the present disclosure. FIG. 5a is a diagram illustrating a bright-field imaging method for the process in which light emitted from a light source unit reaches an object according to one embodiment of the present disclosure. FIG. 5b is a diagram illustrating a bright-field imaging method for the process in which light scattered or reflected from an object reaches an image acquisition unit according to one embodiment of the present disclosure. FIG. 6a is a diagram illustrating a dark-field imaging method for the process in which light emitted from a light source unit reaches an object according to one embodiment of the present disclosure. FIG. 6b is a diagram illustrating a dark-field imaging method for the process in which light transmitted or reflected from an object reaches an image acquisition unit according to one embodiment of the present disclosure. FIG. 7 is a drawing illustrating a bright-field imaging method for acquiring light scattered by an object and incident on an objective lens according to one embodiment of the present disclosure. To clarify the technical concept of the present disclosure, embodiments of the present disclosure will be described in detail with reference to the attached drawings. In describing the present disclosure, detailed descriptions of related known functions or components will be omitted if it is determined that such detailed descriptions would unnecessarily obscure the essence of the present disclosure. Components having substantially the same functional configuration among the drawings have been assigned the same reference numerals and symbols as much as possible, even if they are shown in different drawings. For convenience of explanation, devices and methods will be described together where necessary. Each operation of the present disclosure does not necessarily have to be performed in the order described and may be performed in parallel, selectively, or individually. The terms used in the embodiments of this disclosure have been selected to be as widely used and general as possible, taking into account the functions of this disclosure; however, these terms may vary depending on the intent of those skilled in the art, case law, the emergence of new technologies, etc. Additionally, in specific cases, terms have been selected at the applicant's discretion, and in such cases, their meanings will be described in detail in the description of the relevant embodiments. Therefore, terms used in this specification should be defined not merely by their names,