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KR-20260066096-A - CARBON DIOXIDE BIOCONVERSION PROCESS

KR20260066096AKR 20260066096 AKR20260066096 AKR 20260066096AKR-20260066096-A

Abstract

A CO2 bioconversion method comprises the step of providing a CO2 - containing substrate to a bioreactor, wherein the CO2- containing substrate contains about 5 mole % to about 90 mole % of CO2 ; and the step of fermenting the CO2- containing substrate with acetic acid-producing bacteria having sodium-transferable ATPase. The medium comprises less than about 0.01 gram/liter of yeast extract, less than about 0.01 gram/liter of carbohydrates, a sodium ion concentration provided at a sodium ion supply rate of about 290 to about 8750 μg/gram cell/min, and a pH of about 4 to about 6.9.

Inventors

  • 세나라트네 라이언
  • 프라이스 아벨
  • 비어드 브랜든

Assignees

  • 주펑 바이오 홍콩 리미티드

Dates

Publication Date
20260512
Application Date
20190802
Priority Date
20180808

Claims (20)

  1. A method including the following steps: A step of providing a gaseous substrate to a bioreactor, wherein the gaseous substrate comprises CO₂ and contains about 5 mole % to about 90 mole % of CO₂ ; Step of providing acetic acid-producing bacteria to a bioreactor; A step of providing sodium ions to a bioreactor through one or more sodium ion sources; and A step of producing one or more organic acids by fermenting a gaseous substrate with acetic acid-producing bacteria in a fermentation broth containing acetic acid-producing bacteria and one or more sodium ion sources; Acetic acid-producing bacteria contain sodium-transferred ATPase that is active during fermentation in a bioreactor, and The fermented broth contains less than about 0.01 gram/liter of yeast extract and less than about 0.01 gram/liter of carbohydrates, and Sodium ions are provided at a sodium supply rate of about 290 to about 8750 μg/gram cell/min, and The fermented broth is maintained at a pH in the range of about 4 to about 6.9.
  2. A method according to claim 1, wherein the CO2- containing gaseous substrate is selected from the group consisting of industrial gases, fermenter gas streams, and mixtures thereof.
  3. A method according to claim 1, wherein the acetic acid-producing bacteria are selected from the group consisting of Acetobacterium bacteria, Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Moorella thermoacetica, Moorella thermoautotrophica, Ruminococcus productus, and combinations thereof.
  4. In claim 3, the method in which the acetic acid-producing bacteria is Acetobacterium woodii.
  5. A method according to claim 1, wherein the sodium ion source is provided by a compound selected from the group consisting of sodium chloride, sodium hydroxide, sodium phosphate, sodium sulfate, sodium nitrate, sodium bicarbonate, sodium bisulfate, and mixtures thereof.
  6. A method according to claim 1, wherein the organic acid is one or more C1 to C10 organic acids.
  7. In claim 6, the method wherein the organic acid is acetic acid, butyric acid, or a mixture thereof.
  8. A method including the following steps: A step of providing a gaseous substrate to a bioreactor, wherein the gaseous substrate comprises CO₂ and H₂ , and contains about 5 mole % to about 90 mole % of CO₂ ; Step of providing acetic acid-producing bacteria to a bioreactor; A step of providing sodium ions to a bioreactor through one or more sodium ion sources; and A step of producing one or more organic acids by fermenting a gaseous substrate with acetic acid-producing bacteria in a fermentation broth containing acetic acid-producing bacteria and one or more sodium ion sources; Acetic acid-producing bacteria contain sodium-transferred ATPase that is active during fermentation in a bioreactor, and The fermented broth contains less than about 0.01 gram/liter of yeast extract and less than about 0.01 gram/liter of carbohydrates, and Sodium ions are provided at a sodium supply rate of about 290 to about 8750 μg/gram cell/min, and The fermented broth is maintained at a pH in the range of about 4 to about 6.9.
  9. In claim 8, the gaseous substrate is selected from the group consisting of industrial gases, fermenter gas streams, and mixtures thereof.
  10. In claim 8, the acetic acid-producing bacteria are Acetobacterium bacteria, Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Moorella thermoacetica, Moorella thermoautotrophica, Ruminococcus productus, and a method selected from a group consisting of combinations thereof.
  11. In claim 10, the method in which the acetic acid-producing bacteria is Acetobacterium woodii.
  12. A method according to claim 8, wherein the sodium ion source is provided by a compound selected from the group consisting of sodium chloride, sodium hydroxide, sodium phosphate, sodium sulfate, sodium nitrate, sodium bicarbonate, sodium bisulfate, and mixtures thereof.
  13. In claim 8, the method wherein the organic acid is one or more C1 to C10 organic acids.
  14. In claim 13, the method wherein the organic acid is acetic acid, butyric acid, or a mixture thereof.
  15. A composition comprising the following: One or more sources of NH₄⁺ , P , K, Fe, Ni, Co, Se, Zn, W, or Mg; A sodium ion source of about 875 to about 35,000 mg/L; and A source of Mo of about 0.009 to about 0.397 mg/L, The composition comprises less than about 0.01 gram/liter of yeast extract and less than about 0.01 gram/liter of carbohydrates, and The composition has a pH of about 4 to about 6.9.
  16. A composition according to claim 15, wherein the sodium ion source is provided by a compound selected from the group consisting of sodium chloride, sodium hydroxide, sodium phosphate, sodium sulfate, sodium nitrate, sodium bicarbonate, sodium bisulfate, and mixtures thereof.
  17. A composition according to claim 15, wherein the composition comprises a complexing agent selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), ethylenediaminediacetic acid (EDDA), ethylenediaminedisuccinic acid (EDDS), and mixtures thereof.
  18. In claim 15, the composition comprises the following: NH4 + source of about 82 to about 3280 mg/L; A phosphorus source of about 20.12 to about 805 mg/L; or Potassium source of about 98.33 to about 3933 mg/L.
  19. In claim 18, the nitrogen is provided from a nitrogen source selected from the group consisting of ammonium hydroxide, ammonium chloride, ammonium phosphate, ammonium sulfate, ammonium nitrate, and mixtures thereof; Phosphorus is provided from a phosphorus source selected from the group consisting of phosphoric acid, ammonium phosphate, potassium phosphate, and mixtures thereof; Potassium is provided from a potassium source selected from the group consisting of potassium chloride, potassium phosphate, potassium nitrate, potassium sulfate, and mixtures thereof. Composition.
  20. In claim 19, the composition comprises the following: An iron source of about 0.85 to about 34 mg/L; A nickel source of about 0.07 to about 2.81 mg/L; A cobalt source of about 0.037 to about 1.49 mg/L; A selenium source of about 0.027 to about 1.1 mg/L; A zinc source of about 0.59 to about 23.8 mg/L; A tungsten source of about 80.25 to about 3210 mg/L; or A magnesium source of about 0.71 to about 28.69 mg/L.

Description

Carbon Dioxide Bioconversion Process This application claims priority to U.S. provisional applications No. 62/716,083 (filed August 8, 2018), No. 62/716,071 (filed August 8, 2018), No. 62/716,053 (filed August 8, 2018), No. 62/741,871 (filed October 5, 2018) and No. 62/741,797 (filed October 5, 2018), all of which are incorporated herein by reference in their entirety. A method for the bioconversion of carbon dioxide is provided. More specifically, the method comprises providing a carbon dioxide-containing gas stream to acetic acid-producing bacteria. The method provides a high level of carbon dioxide conversion and hydrogen utilization. Carbon dioxide generation occurs from natural processes as well as industrial processes, including the combustion of fossil fuels such as coal, oil, and natural gas. Partly due to industrial processes, the concentration of carbon dioxide in the atmosphere continues to increase. This increase in carbon dioxide concentration can contribute to atmospheric changes that lead to climate change and global warming. Due to its highly oxidized state, carbon dioxide is difficult to utilize in biological processes. In addition to carbon dioxide, many industrial processes also result in the production of hydrogen. Hydrogen possesses a high level of reducing power. However, due to its highly flammable nature, hydrogen is difficult to store and utilize. Considering the generation of large amounts of carbon dioxide, a bacterial fermentation system capable of reducing carbon dioxide emissions is required. Additionally, a fermentation system capable of effectively utilizing the reducing power of hydrogen is necessary. The method comprises providing a gaseous substrate to a bioreactor. The gaseous substrate contains CO₂ and contains about 5 mole% to about 90 mole% of CO₂ . The method comprises the steps of providing acetic acid-producing bacteria to a bioreactor; providing sodium ions to the bioreactor through one or more sodium ion sources; and fermenting the gaseous substrate with acetic acid-producing bacteria in a fermentation broth comprising acetic acid-producing bacteria and one or more sodium ion sources to produce one or more organic acids. The acetic acid-producing bacteria contain sodium-transferable ATPases that are active during fermentation in the bioreactor. The fermentation broth comprises less than about 0.01 gram/liter of yeast extract, less than about 0.01 gram/liter of carbohydrates, and a sodium ion concentration provided by a sodium supply rate of about 290 to about 8750 μg/g cell/min. The fermentation broth is maintained at a pH in the range of about 4 to about 6.9. In another embodiment, the method comprises providing a gaseous substrate to a bioreactor. The gaseous substrate comprises CO₂ and H₂ and contains about 5 mole% to about 90 mole% of CO₂ . The method comprises the steps of providing acetic acid-producing bacteria to a bioreactor; providing sodium ions to the bioreactor through one or more sodium ion sources; and fermenting the gaseous substrate with the acetic acid-producing bacteria in a fermentation broth comprising the acetic acid-producing bacteria and one or more sodium ion sources to produce one or more organic acids. The acetic acid-producing bacteria contain sodium-potential ATPases that are active during fermentation in the bioreactor. The fermentation broth comprises less than about 0.01 gram/liter of yeast extract, less than about 0.01 gram/liter of carbohydrates, and a sodium ion concentration provided by a sodium supply rate of about 290 to about 8750 μg/g cell/min. The fermentation broth is maintained at a pH in the range of about 4 to about 6.9. The composition comprises one or more sources of NH₄⁺, P, K, Fe, Ni, Co, Se, Zn, W, or Mg; a sodium ion source of about 875 mg/L to about 35,000 mg/L; and a Mo source of about 0.009 mg/L to about 0.397 mg/L. The composition comprises less than about 0.01 gram/L of yeast extract and less than about 0.01 gram/L of carbohydrate. The composition has a pH of about 4 to about 6.9. To enable a detailed understanding of the aforementioned features of the present invention, a more specific description of the invention, as briefly summarized above, may be obtained by referring to embodiments, some of which are illustrated in the accompanying drawings. However, it should be noted that the accompanying drawings illustrate only typical embodiments of the present invention and should not be construed as limiting the scope thereof, because other equally effective embodiments of the present invention may be acknowledged. Figure 1 shows a graph of CO2 conversion and H2 conversion by Acetobacterium woodii in a bioreactor. Figure 2 illustrates the production of acetic acid by Acetobacterium woodii. Figure 3 illustrates the growth of Acetobacterium woodii in the presence of 5% CO₂. Figure 4 illustrates the growth of Acetobacterium woodii in the presence of 5% CO₂. Figure 5 illustrates the CO2 conversion, H2 conversion, and