KR-20260066888-A - BIOLOGICAL MATERIAL INFORMATION GENERATION DEVICE AND METHOD THEREOF

KR20260066888AKR 20260066888 AKR20260066888 AKR 20260066888AKR-20260066888-A

Abstract

The present disclosure relates to an apparatus and method for generating bio-sample information, comprising at least one memory including computer program instructions and at least one processor for executing computer program instructions, wherein the at least one processor performs light source control for irradiating light onto a bio-sample contained in a sample container using a light source, performs detection control for detecting a light signal using a photodetector based on diffuse light for the incident light, and generates bio-sample information regarding the bio-sample based on the light signal.

Inventors

김철
김경남
황보용훈

Assignees

한국전기연구원

Dates

Publication Date: 20260512
Application Date: 20241105

Claims (14)

In a bio-sample information generation device, At least one memory containing computer program instructions; and It includes at least one processor that executes the above computer program instructions, The above at least one processor is, Light source control is performed to irradiate light onto a bio-sample contained in a sample container using a light source, and Detection control is performed to detect an optical signal using a photodetector based on diffuse light of the incident light, and A bio-sample information generating device that generates bio-sample information regarding the bio-sample based on the above-mentioned optical signal.
In paragraph 1, The above bio sample information is, A bio-sample information generating device comprising at least one of quantitative information regarding the amount of the bio-sample and active information regarding the activity of the bio-sample.
In paragraph 2, The above light detector is, It includes a first photodetector that detects a first photosignal based on diffuse light in which the incident light passes through the bio sample in an amount less than the critical passing amount, and a second photodetector that detects a second photosignal based on diffuse light in which the incident light passes through the bio sample in an amount greater than the critical passing amount. The above processor is, A bio-sample information generation device that performs balanced detection control using the signal difference between the first optical signal and the second optical signal.
In paragraph 2 The above processor is, A bio-sample information generating device that determines an autocorrelation function based on the above-mentioned optical signal, determines an optical characteristic value based on the above-mentioned autocorrelation function, and generates the above-mentioned quantitative information based on the above-mentioned optical characteristic value.
In paragraph 2, The above processor is, A bio sample information generating device that further performs temperature control by controlling a thermoelectric element installed near the sample container so that the temperature of the bio sample reaches a preset target temperature.
In paragraph 5, The above processor is, A bio-sample information generation device that performs PID feedback temperature control based on temperature difference information between the temperature of the bio-sample and the target temperature.
In paragraph 5, The above processor is, A bio sample information generation device that, when the temperature of the bio sample reaches the target temperature through the above temperature control, sets the active information as active if the rate of change of the quantitative information is greater than or equal to a preset threshold rate of change, and sets the active information as inactive if the rate of change of the quantitative information is less than a preset threshold rate of change.
A light source control step for irradiating light onto a bio-sample contained in a sample container using a light source; A detection control step for controlling the detection of an optical signal using a photodetector based on diffuse light of the incident light; and A method for generating bio-sample information comprising an information generation step of generating bio-sample information regarding the bio-sample based on the above-mentioned optical signal.
In paragraph 8, The above bio sample information is, A method for generating bio-sample information comprising at least one of quantitative information regarding the amount of the bio-sample and active information regarding the activity of the bio-sample.
In Paragraph 9, The above light detector is, It includes a first photodetector that detects a first photosignal based on diffuse light in which the incident light passes through the bio sample in an amount less than the critical passing amount, and a second photodetector that detects a second photosignal based on diffuse light in which the incident light passes through the bio sample in an amount greater than the critical passing amount. The above detection control step is, A method for generating bio-sample information comprising performing balanced detection control utilizing the signal difference between the first optical signal and the second optical signal.
In Paragraph 9, The above information generation step is, A method for generating bio-sample information, comprising determining an autocorrelation function based on the above-mentioned optical signal, determining an optical characteristic value based on the above-mentioned autocorrelation function, and generating the above-mentioned quantitative information based on the above-mentioned optical characteristic value.
In Paragraph 9, A method for generating bio-sample information, further comprising a temperature control step of controlling a thermoelectric element installed near the sample container so that the temperature of the bio-sample reaches a preset target temperature.
In Paragraph 12, The above temperature control step is, A method for generating biosample information, comprising performing PID feedback temperature control based on temperature difference information between the temperature of the biosample and the target temperature.
In Paragraph 12, The above information generation step is, A method for generating bio sample information, comprising: when the temperature of the bio sample reaches the target temperature through the above temperature control, setting the active information as active if the rate of change of the quantitative information is greater than or equal to a preset threshold rate of change, and setting the active information as inactive if the rate of change of the quantitative information is less than the preset threshold rate of change.

Description

Biological Material Information Generation Device and Method Thereof The present disclosure relates to an apparatus and method for generating bio-sample information, and more specifically, to an apparatus and method for generating bio-sample information based on an optical signal. Bio-samples may contain cells, microorganisms, or biomolecules, all of which possess unique characteristics in that they are microscopic in size, difficult to identify with the naked eye, and can react sensitively to environmental factors such as temperature and humidity. In addition, when only trace amounts of bio-samples are present, conventional detection and analysis techniques have the problem of requiring a long time for the sample to grow sufficiently, or making it difficult to accurately detect and analyze. FIG. 1 is a diagram illustrating the configuration of a bio-sample detection system according to the present disclosure in an exemplary manner. FIG. 2 is a block diagram of a computing system according to the present disclosure. FIG. 3 is a graph illustrating a configuration for performing detection control using an autocorrelation function according to one embodiment. FIG. 4 is a diagram illustrating a configuration for performing detection control using a balanced type detection according to one embodiment. FIG. 5 is a diagram illustrating a configuration for performing temperature control according to one embodiment. FIG. 6 is a graph illustrating a configuration for performing PCR temperature control in temperature control according to one embodiment. FIG. 7 is a diagram illustrating a configuration for performing feedback control in temperature control according to one embodiment. FIG. 8 is a graph illustrating a configuration for generating active information in information generation according to one embodiment. FIG. 9 is a flowchart illustrating a method for generating bio-sample information according to one embodiment. FIG. 10 is a flowchart illustrating a process for generating bio-sample information including temperature control according to one embodiment. Hereinafter, some embodiments of the present disclosure will be described in detail with reference to the exemplary drawings. In assigning reference numerals to the components of each drawing, the same components may have the same reference numeral as much as possible, even if they are shown in different drawings. Furthermore, in describing the embodiments, if it is determined that a detailed description of related known components or functions may obscure the essence of the technical concept, such detailed description may be omitted. Where terms such as "comprising," "having," or "consisting of" are used in this specification, other parts may be added unless "only" is used. Where a component is expressed in the singular, it may include a plural unless otherwise specified. Additionally, terms such as first, second, A, B, (a), (b), etc., may be used to describe the components of the present disclosure. These terms are used merely to distinguish the components from other components, and the nature, order, sequence, or number of the components are not limited by such terms. In describing the positional relationship of components, where it is stated that two or more components are "connected," "combined," or "joined," it should be understood that while the two or more components may be directly "connected," "combined," or "joined," they may also be "connected," "combined," or "joined" with other components "intervened." Here, the other components may be included in one or more of the two or more components that are "connected," "combined," or "joined" with one another. In describing the temporal flow relationship regarding components, methods of operation, or methods of production, for example, when the temporal or sequential relationship is described using "after," "following," "next," or "before," it may include cases where the relationship is not continuous unless "immediately" or "directly" is used. Meanwhile, where numerical values or corresponding information regarding a component (e.g., levels, etc.) are mentioned, even without separate explicit notation, the numerical values or corresponding information may be interpreted as including a range of error that may occur due to various factors (e.g., process factors, internal or external shocks, noise, etc.). FIG. 1 is a diagram illustrating the configuration of a bio-sample detection system according to the present disclosure in an exemplary manner. Referring to FIG. 1, a bio sample detection system according to the present disclosure may include an integrated control device (110), a light measuring device (120), a light source (122), a first light detector (124), a second light detector (126), a sample container (130), a structure (132), a temperature control device (140), a temperature sensor (142), and a thermoelectric element (144). Each component of the bio sample detection system may be physically or