KR-20260067405-A - Peptide for antimicrobial, anti-inflammatory, anti-acne, improvement of skin itching or moisturizing and cosmetic composition for comprising the same

Abstract

The present invention relates to a peptide for antibacterial, anti-inflammatory, anti-acne, skin itching improvement, or skin moisturization, wherein the peptide is a peptide in which zinc is chelated to the amino acid sequence of SEQ ID NO. 1 or 2, and has antibacterial, anti-inflammatory, anti-acne, skin itching improvement, or skin moisturization effects, and can be utilized as a cosmetic composition containing the same.

Inventors

• 한상철
• 장소영
• 이정임
• 박혜린

Assignees

• (주)더마펌

Dates

Publication Date

20260513

Application Date

20241104

Claims (9)

1. A peptide for antibacterial, anti-inflammatory, anti-acne, improvement of skin itching, or skin moisturization, comprising a peptide composed of the amino acid sequence of SEQ ID NO. 1 or 2, wherein zinc (Zn) is bonded to said peptide.
2. A peptide according to claim 1, wherein the zinc is chelated with alanine (Ala) and histidine (His) of a peptide composed of the amino acid sequence of SEQ ID NO. 1.
3. A peptide according to claim 1, wherein the zinc is chelated with glycine (Gly) and histidine (His) of a peptide consisting of the amino acid sequence of SEQ ID NO. 2.
4. A peptide according to claim 1, wherein the bacterium is Cutibacterium acnes.
5. A peptide according to claim 1, wherein the anti-inflammatory effect is due to the inhibition of the production of IL-6 or IL-8.
6. A cosmetic composition for antibacterial, anti-inflammatory, or anti-acne use comprising any one of the peptides of claims 1 to 5.
7. A cosmetic composition for improving skin itching comprising any one of the peptides of claims 1 to 5.
8. A cosmetic composition according to claim 7, wherein the skin itching is caused by skin dryness.
9. A cosmetic composition for moisturizing the skin comprising any one of the peptides of claims 1 to 5.

Description

Peptide for antimicrobial, anti-inflammatory, anti-acne, improvement of skin itching or moisturizing and cosmetic composition for comprising the same The present invention relates to a peptide for antibacterial, anti-inflammatory, anti-acne, improvement of skin itching, or skin moisturization, and a cosmetic composition containing the same. The human skin functions as a protective barrier that shields the body from the external environment. It plays a role in preventing the loss of biological components, such as water and electrolytes, while simultaneously blocking harmful substances from entering the body. Furthermore, it must prevent moisture loss from the body and maintain flexibility by retaining an adequate amount of moisture itself. To this end, ingredients similar to sebum, natural moisturizing factors (NMFs), or moisturizers such as polyols have been added to cosmetics. However, water-soluble polyols with three or more hydroxyl groups (OH groups), such as glycerin and solitol, exhibit excellent moisturizing power but cause discomfort due to excessive stickiness during use, while propylene glycol and 1,3-butylene glycol with two hydroxyl groups (OH groups) can cause skin side effects. Furthermore, natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea have strong electrolytic properties that compromise the emulsion stability of cosmetics. In addition, amino acids, collagen, and elastin are also known to possess moisturizing abilities, but they have limitations in their moisturizing capacity. Therefore, the development of substances that are safe, highly usable, and have high moisturizing effects is one of the important research tasks in cosmetics. Meanwhile, antimicrobial activity refers to the inhibition of the growth of bacteria and fungi by drugs or physiologically active substances, and is broadly classified into bactericidal and bacteriostatic types. Among substances possessing antimicrobial action, antibiotics are considered the most important. The range of microorganisms that an antibiotic can act upon is called the antimicrobial spectrum or antimicrobial range, and antibiotics with a broad antimicrobial spectrum are called broad-spectrum antibiotics. Tetracycline antibiotics and cephalosporin antibiotics fall into this category. While it is a well-known fact that most bacterial infections have been suppressed with the advent of antibiotics, the emergence and proliferation of drug-resistant bacteria that resist antimicrobial action pose serious problems for treatment. Meanwhile, inflammation refers to a complex lesion characterized by the concomitant occurrence of three factors: tissue degeneration, circulatory disorders and exudation, and tissue proliferation, as one of the biological tissue's defensive responses to stimuli. It can also be described as the manifestation of an in vivo defense mechanism against various forms of infection or irritating substances among metabolic products. Various chemical mediators are involved in the mechanism of inflammation, and its etiology is highly complex. When inflammation occurs, microvessels are perforated, blood components leak into the interstitial spaces, and leukocytes migrate to the inflamed tissue; this is typically accompanied by clinical symptoms such as erythema, edema, hyperalgesia, and pain. Various biochemical phenomena are involved in the causes of inflammation in living organisms, and in particular, nitric oxide synthase (NOS), an enzyme that generates nitric oxide (NO), and enzymes involved in the biosynthesis of prostaglandins are known to play an important role in mediating inflammatory responses. However, widely known anti-inflammatory materials include organic compounds (heterocyclic or non-cyclic compounds), herbal extracts, enzymes, peptides, sugars, steroids, and oils, but they have disadvantages such as poor stability in formulations due to physicochemical instability, concerns about side effects from long-term use due to the nature of dermatitis, and potential skin irritation such as secondary infection caused by atopy. Therefore, there is an urgent need to develop an ingredient that is safe for the body, has stable active ingredients, and, above all, is more effective than existing substances with anti-inflammatory, antibacterial, anti-acne, anti-itch, and moisturizing properties. Figure 1 confirms the cytotoxicity of Zn-AHKRRRR and Zn-GHKRRRR peptides. Figures 2 and 3 confirm the inflammatory factor reduction effect of Zn-AHKRRRR and Zn-GHKRRRR peptides. Figure 4 confirms the inhibitory activity of Zn-AHKRRRR and Zn-GHKRRRR peptides against acne-causing bacteria ( C. acnes ). Figure 5 confirms the inhibition of the formation of biofilms causing antibiotic resistance by Zn-AHKRRRR and Zn-GHKRRRR peptides. Figure 6 shows the change in maximum and mean itching scores after applying a cream containing Zn-AHKRRRR and Zn-GHKRRRR peptides for 2 consecutive weeks (mean ± SD, *p < 0.05 ba