KR-20260067414-A - Method for diagnosing of non-infectious meningoencephalomyelitis in dog using S100B protein expression level and composition therefor

Abstract

The present invention relates to a method for diagnosing non-infectious meningitis in dogs using the S100B protein expression level and a composition for the same. It is expected that using the method of the present invention will enable easy, fast, and simple diagnosis of non-infectious meningitis in dogs.

Inventors

• 송중현
• 김보경

Assignees

• 충남대학교산학협력단

Dates

Publication Date

20260513

Application Date

20241104

Claims (9)

1. (1) Step of isolating a specimen from a dog suspected of having non-infectious meningitis; (2) A step of measuring the concentration of S100B protein in the separated sample; and (3) A step of diagnosing non-infectious meningitis when the concentration of the S100B protein is greater than or equal to a cutoff value; comprising a method for diagnosing non-infectious meningitis in dogs.
2. A method for diagnosing non-infectious meningitis in dogs according to claim 1, characterized in that the non-infectious meningitis is meningoencephalitis of unknown origin.
3. A method for diagnosing non-infectious meningitis in dogs, characterized in that, in claim 1, the specimen is cerebrospinal fluid or blood.
4. A method for diagnosing non-infectious meningitis in dogs according to claim 1, characterized in that the S100B protein is composed of the amino acid sequence of SEQ ID NO. 1.
5. A method for diagnosing non-infectious meningitis in dogs, characterized in that, in claim 1, the cutoff value is 830 to 980 pg/㎖.
6. A method for diagnosing non-infectious meningitis in dogs, characterized in that, in claim 5, the cutoff value is 835.82 pg/mL when the sample in step (1) is cerebrospinal fluid, and the cutoff value is 977.06 pg/mL when it is blood.
7. A composition for diagnosing non-infectious meningitis in dogs, comprising a preparation for measuring the expression level of S100B protein consisting of the amino acid sequence of SEQ ID NO. 1.
8. A composition for diagnosing non-infectious meningitis in dogs according to claim 7, wherein the preparation for measuring the S100B protein expression level comprises an antibody or aptamer specific to said protein.
9. A kit for diagnosing non-infectious meningitis in dogs comprising the composition of claim 7 or 8.

Description

Method for diagnosing of non-infectious meningoencephalomyelitis in dog using S100B protein expression level and composition therefor The present invention relates to a method for diagnosing non-infectious meningitis in dogs using S100B protein expression levels and a composition for the same. Non-infectious central nervous system diseases are common in dogs and can progress to serious neurological problems if not treated immediately. Non-infectious central nervous system diseases in dogs can be broadly classified into steroid-responsive meningitis-arteritis and meningoencephalitis of unknown origin; while steroid-responsive meningitis-arteritis can be easily diagnosed based on specific clinical symptoms, diagnosing meningoencephalitis of unknown origin is not easy. Meningoencephalitis of unknown origin in dogs is a representative intractable brain disease that collectively refers to clinically indistinguishable autoimmune central nervous system diseases. It is known to account for about one-quarter of all neurological patients visiting veterinary hospitals in Korea, and the average life expectancy after diagnosis is reported to be approximately 1 to 2 years. Much is unknown regarding the fundamental etiology and pathogenesis of meningitis of unknown etiology. Since a provisional diagnosis is made via magnetic resonance imaging (MRI) and an invasive brain biopsy is essential for final confirmation, it is difficult to reach a definitive diagnosis in general veterinary clinics, resulting in significant medical expenses during the diagnostic process. Furthermore, MRI scans require anesthesia, and it is known that elevated cerebral pressure caused by anesthesia can worsen symptoms in animals suffering from meningitis of unknown etiology. Therefore, there is a pressing need for research into easier and safer methods for diagnosing meningitis of unknown etiology in dogs. Meanwhile, Korean Published Patent No. 2020-0083371 discloses a 'method for providing information for diagnosing meningitis' using a vitamin D binding protein, and Korean Registered Patent No. 1592854 discloses a 'biomarker composition for diagnosing hydrocephalus containing Anks1a protein,' but there is no description regarding the 'method for diagnosing non-infectious meningitis in dogs using S100B protein expression levels and a composition for the same' of the present invention. Figure 1 shows the results of measuring the concentration of S100B protein in cerebrospinal fluid (A) and serum (B) isolated from healthy dogs (Control), dogs with meningoencephalitis of unknown origin (MUO), and dogs with idiopathic epilepsy (IE). CSF: cerebrospinal fluid. Figure 2 shows the results of evaluating the correlation between the concentration of S100B protein in cerebrospinal fluid and the concentration of S100B protein in serum using Spearman's correlation analysis with S100B protein concentrations in cerebrospinal fluid and serum isolated from healthy dogs, dogs with meningitis of unknown etiology, and dogs with idiopathic epilepsy. r s : Spearman's rank correlation coefficient. Figure 3 is a Receiver Operating Characteristic (ROC) curve analyzed using the concentration of S100B protein in cerebrospinal fluid (A) and serum (B) isolated from healthy dogs, dogs with meningitis of unknown etiology, and dogs with idiopathic epilepsy. The vertical axis represents sensitivity, and the horizontal axis represents specificity. To achieve the objective of the present invention, the present invention provides a method for diagnosing non-infectious meningitis in dogs, comprising: (1) a step of isolating a specimen from a dog suspected of having non-infectious meningitis; (2) a step of measuring the concentration of S100B protein in the isolated specimen; and (3) a step of diagnosing non-infectious meningitis when the concentration of S100B protein is greater than or equal to a cutoff value. In the method for diagnosing non-infectious meningitis in dogs according to the present invention, the non-infectious meningitis may preferably be meningoencephalitis of unknown origin, but is not limited thereto. In the method for diagnosing non-infectious meningitis in dogs according to the present invention, the specimen may be cerebrospinal fluid or blood, but is not limited thereto. In the method for diagnosing non-infectious meningitis in dogs according to the present invention, the S100B protein may preferably be composed of the amino acid sequence of SEQ ID NO. 1, but is not limited thereto. In addition, the S100B protein comprises a functional equivalent of a protein having the amino acid sequence represented by SEQ ID NO. 1. In the present invention, the term "functional equivalent" refers to a protein that exhibits substantially the same physiological activity as the protein represented by SEQ ID NO. 1, having at least 70%, preferably 80%, more preferably 90%, and most preferably 95% sequence homology with the amino acid sequence represented by SEQ ID NO. 1 as a resul