KR-20260067829-A - Cosmetic composition containing strawberry(Fragaria x ananassa) root extract as an effective ingredient and its manufacturing method

Abstract

The present invention relates to a cosmetic composition containing strawberry root extract as an active ingredient, and more specifically, to the antioxidant, anti-inflammatory, and whitening effects of the extract of the strawberry root portion. A cosmetic composition containing strawberry root extract as an active ingredient according to the present invention can confirm the anti-inflammatory efficacy of the strawberry root extract in macrophages activated by LPS, and confirm the antioxidant effect through electron donating ability and ABTS radical scavenging ability. In addition, by confirming tyrosinase inhibitory activity and melanin biosynthesis inhibitory activity, the whitening effect can be enhanced, and thus it can be utilized as a cosmetic composition.

Inventors

• 유단희
• 김정환
• 이인철
• 강정욱

Assignees

• 서원대학교산학협력단

Dates

Publication Date

20260513

Application Date

20241106

Claims (3)

1. A cosmetic composition containing strawberry root extract as an active ingredient, characterized by containing strawberry root 70% ethanol extract as an active ingredient and having antioxidant, anti-inflammatory activity and whitening efficacy.
2. In paragraph 1, The above extract is, It has tyrosinase inhibitory activity, Having DPPH radical scavenging ability and ABTS radical scavenging ability, It has inhibitory activity on nitric oxide production in macrophages activated by LPS, and A cosmetic composition containing strawberry root extract as an active ingredient, characterized in that the above extract has a concentration of 500 μg/ml.
3. In paragraph 2, The above extract is, A step of washing strawberry roots and then hot-air drying them at 60℃ for 15 hours; The step of crushing the dried strawberry roots mentioned above; A step of adding 10 parts by weight of 70% ethanol to 1 part by weight of the crushed strawberry root and stirring at room temperature for 24 hours; and The method includes a step of separating the supernatant and the precipitate through primary filtration using a nonwoven fabric after the above stirring step; A second step of adding 10 parts by weight of 70% ethanol based on 1 part by weight of the precipitate obtained from the above stirring step and the step of separating the supernatant and precipitate, and stirring and separating the supernatant and precipitate; and A third step of adding 10 parts by weight of 70% ethanol based on 1 part by weight of the precipitate obtained from the second step above, and stirring and separating the supernatant and the precipitate; further comprising A step of secondary filtration of the supernatant obtained from hot water and 70% ethanol extraction using filter paper and a vacuum pump; A step of concentrating the supernatant of the second filtered liquid using a rotary evaporator under reduced pressure; and A method for preparing a cosmetic composition containing strawberry root extract as an active ingredient, characterized by further including the step of freeze-drying the concentrate concentrated under reduced pressure as described above.

Description

Cosmetic composition containing strawberry (Fragaria x ananassa) root extract as an effective ingredient and its manufacturing method The present invention relates to a cosmetic composition containing strawberry root extract as an active ingredient, and more specifically, to the antioxidant, anti-inflammatory, and whitening effects of the extract of the strawberry root portion. In modern society, rapid advancements in science and technology have led to increased life expectancy and improved living standards, resulting in a growing interest in beauty alongside health. In particular, due to environmental pollution, ozone depletion, and various chemical and synthetic substances, there is increasing interest in superior natural materials that cause fewer side effects on the skin. The epidermis is located at the outermost layer of the skin and possesses a barrier function to protect against the influx of various external factors, such as ultraviolet rays, viruses, and harmful substances. It is known to primarily contain keratinocytes, Langerhans immune cells, and melanocytes. When the skin is subjected to continuous stimuli—such as external factors like ultraviolet rays and environmental pollution, or internal factors like aging and mental stress—reactive oxygen species (ROS) are generated, leading to oxidative stress. This oxidative stress causes the oxidation of proteins or DNA, lipid peroxidation, and the destruction of skin antioxidants. Consequently, the skin barrier function fails to operate smoothly, resulting in harmful effects such as chronic inflammatory diseases, increased melanin production, and pigmentation caused by premature aging. The inflammatory response is an essential mechanism for repairing and regenerating damaged tissues and the body by preventing damage from physical and chemical stimuli and secreting various inflammatory mediators. Reactive oxygen species, the primary cause of oxidative stress, promote the secretion of cytokines such as tumor necrosis factor-α, interleukin 6, and IL-1β (Kader & Coyle, 2007; Yayeh et al., 2012), as well as inflammation-related mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2). In particular, NO is produced by inducible nitric oxide synthase, and excessive production leads to inflammation, genetic mutations, and tissue and nerve damage. Additionally, PGE2 is produced by cyclooxygenase-2 and acts as an inflammatory factor that causes pain and fever. α-melanocyte-stimulating hormone (α-MSH), secreted when skin is exposed to ultraviolet rays, activates various intracellular signals and factors and induces the expression of tyrosinase by increasing the expression of microphthalmia-associated transcription factor, a key transcription regulator in the melanin synthesis process. Tyrosinase, a key enzyme that produces melanin polymers, converts L-tyrosine into L-dihydroxyphenylalanine (L-DOPA), L-DOPA into DOPA quinone, and DOPA quinone into dopachrome through auto-oxidation and enzymatic reactions to produce melanin, a dark brown copolymer. However, since excessive melanin production in the skin can cause pigmentation, melasma, freckles, and skin cancer, research is continuously being conducted on the possibility of inducing a whitening effect by inhibiting tyrosinase activity to suppress melanin synthesis. Meanwhile, the strawberry (Fragaria x ananassa) is a perennial plant belonging to the Rosaceae family. Although it is a crop that requires a long cultivation period and a lot of labor, it can grow at low temperatures and can be harvested from November to May through low-heat facility cultivation. While the composition of strawberries varies depending on the variety, they are rich in sugars and organic acids and contain functional components such as vitamin C, quercetin, ferulic acid, and flavonols. Strawberries, which contain various components, have antioxidant activity that is about 2 to 11 times higher than that of apples, peaches, pears, grapes, tomatoes, oranges, and kiwis. It has been reported that strawberry calyx extract and hot water and 70% ethanol extracts of strawberry stems are effective for antioxidant function, inhibition of age-related degenerative diseases, and soothing of skin exposed to ultraviolet rays, and have superior whitening effects compared to existing whitening ingredients, along with high melanin inhibitory activity in cells. As such, while research on the bioactivity of various parts of the strawberry is ongoing, the verification of the bioactivity of strawberry root extracts is currently insufficient; therefore, this study aimed to verify the antioxidant, anti-inflammatory, and whitening efficacy of strawberry root extracts to utilize them as a natural cosmetic ingredient. FIG. 1 is a graph briefly illustrating the electron donor measurement results in a cosmetic composition containing strawberry root extract as an active ingredient according to an embodiment of the present invention, where the gray bar graph repres