RU-2024132847-A - RESTORATION OF FERTILITY IN PLANTS

Inventors

• АЛБЕРТСЕН, Марк С
• ФОКС, Тим
• СИНГХ, Манджит
• УИЛЛЬЯМС, Марк И

Assignees

• ПАЙОНИР ХАЙ-БРЕД ИНТЕРНЭШНЛ, ИНК.

Dates

Publication Date

20260508

Application Date

20241101

Priority Date

20180914

Claims (20)

1. 1. A wheat plant cell comprising a homozygous mutation in the male fertility polynucleotide Ms45 of the wheat genomes A, B and D that causes a male sterility phenotype, and a donor chromosomal component that ensures restoration of plant fertility, comprising a chromosomal component 4E from Thinopyrum or Agropyron, wherein the chromosomal component 4E comprises: a plant polynucleotide that ensures a plant seed phenotype linked to a locus that ensures restoration of male fertility mediated by Ms45, which functionally complements the male sterility phenotype from homozygous mutations of Ms45 when expressed in a wheat plant with Ms45-mediated male sterility.
2. 2. A wheat plant cell according to claim 1, wherein the plant polynucleotide that confers the phenotype to the plant seed is located on the same chromosomal arm of chromosomal component 4E as the locus that provides for the restoration of male fertility mediated by Ms45, and they are not separated by a centromere.
3. 3. The wheat plant cell of claim 1, wherein the plant polynucleotide that confers the phenotype to the plant seed is a phenotype related to the color, physiology, or morphology of the seed.
4. 4. A wheat plant cell according to claim 3, wherein the color is represented by blue aleurone, gene P, anthocyanin, or Kala 4.
5. 5. A wheat plant cell according to claim 1, wherein the donor chromosomal component that ensures restoration of plant fertility is from a plant species other than a wheat species.
6. 6. A wheat plant cell according to claim 1, wherein the donor chromosomal component that ensures restoration of plant fertility is derived from a Sebesta Blue, Blue Sando, Blue Baart, Blue Onas, Blue 1, PBB or Blue Norco wheat line containing a donor chromosomal component that ensures restoration of plant fertility, wherein the donor chromosomal component that ensures restoration of plant fertility is derived from a plant other than a wheat plant.
7. 7. A wheat plant obtained from a wheat plant cell according to claim 1.
8. 8. A wheat seed from a plant according to claim 7, wherein the wheat seed expressing the phenotype of the plant seed indicates that the seed will grow into a male-fertile wheat plant after sowing.
9. 9. A wheat seed obtained as a result of self-pollination of a plant according to claim 7, wherein the wheat seed containing homozygous mutations in the Ms45 polynucleotide, which ensures male fertility, of the wheat genomes A, B and D, and not containing the plant polynucleotide, which ensures the phenotype of the plant seed, indicates that a female wheat plant with male sterility will grow from the seed after sowing, wherein the wheat seed containing a homozygous mutation in the Ms45 polynucleotide, which ensures male fertility, of the wheat genomes A, B and D, and the plant polynucleotide, which ensures the phenotype of the plant seed, indicates that a wheat plant with male fertility will grow from the seed after sowing.
10. 10. A method for producing wheat seeds that enable the production of female wheat plants with male sterility, wherein the method comprises:
11. a. sowing a mixture of wheat seeds in a field, where the mixture contains a first part of wheat seeds containing homozygous mutations in the Ms45 polynucleotide that ensures male fertility, wheat genomes A, B and D, which ensure male sterility of the wheat plant, and a second part of wheat seeds containing a donor chromosomal component that ensures the restoration of plant fertility, including the chromosomal component 4E from Thinopyrum or Agropyron, where the chromosomal component 4E, including the plant polynucleotide, which ensures the phenotype of the plant seed, linked to the locus that ensures the restoration of male fertility mediated by Ms45, where the locus that ensures the restoration of male fertility mediated by Ms45, functionally complements the phenotype that ensures male sterility, from homozygous mutations of Ms45 in the wheat plant with male sterility;
12. b. growing plants from a mixture of wheat seeds, wherein the wheat plants with male sterility mediated by Ms45 are the female parent wheat plants, and wherein the wheat plants containing the chromosomal component 4E are the male parent plants;
13. c. ensuring pollination by male parent plants of female parent plants of wheat with male sterility mediated by Ms45, with the production of wheat seeds containing homozygous mutations in the Ms45 polynucleotide providing male fertility, and wheat seeds containing homozygous mutations in the Ms45 polynucleotide providing male fertility, wheat genomes A, B and D, and a donor chromosomal component providing restoration of plant fertility.
14. 11. The method according to claim 10, including sowing a mixture of wheat seeds in a row in a field.
15. 12. The method according to claim 10, including sowing a mixture of wheat seeds in the same row in a field.
16. 13. The method of claim 10, wherein at least about 20%, 25%, 30%, 35%, 40%, or 45% of the resulting wheat seeds are wheat seeds containing homozygous mutations of the male fertility polynucleotide Ms45 and chromosomal component 4E.
17. 14. The method of claim 10, wherein at least about 55%, 60%, 65%, 70%, 75%, or 80% of the resulting wheat seeds are wheat seeds containing homozygous mutations of the male fertility polynucleotide Ms45, but not the chromosomal component 4E.
18. 15. The method according to claim 10, wherein the number of wheat seeds containing homozygous mutations of the Ms45 polynucleotide that ensures male fertility is increased compared to the number of wheat seeds containing the chromosomal component 4E and homozygous mutations of the Ms45 polynucleotide that ensures male fertility, obtained in a field where the wheat seeds from which the development of female and male parent plants will occur are planted in separate rows.
19. 16. The method according to claim 10, wherein the plant polynucleotide that provides the phenotype of the plant seed is located on the same chromosomal arm of chromosomal component 4E as the locus that provides restoration of male fertility mediated by Ms45, and they are not separated by a centromere.
20. 17. The method of claim 10, wherein the chromosomal component 4E is from a species other than wheat.