RU-2026100579-A - A METHOD FOR PRODUCING L-ARGININE USING RECOMBINANT ESCHERICHIA COLI AND THE RECOMBINANT BACTERIA USED IN THE METHOD

Inventors

• СЕ, Сисянь
• ЦЗЯН, Шуай
• У, Хэюнь
• МА, Цянь
• ЛИ, Жань
• МЭН, Ган
• ЧЖАО, Чуньгуан
• ВЭЙ, Айин
• ЦЗЯ, Хуэйпин

Assignees

• НИНСЯ ЭППЕН БИОТЕК КО., ЛТД

Dates

Publication Date

20260508

Application Date

20240703

Priority Date

20230718

Claims (20)

1. 1. A recombinant enterobacterium, wherein said recombinant enterobacterium comprises a recombinant strain obtained by overexpressing a gene encoding a cyanate transporter and/or overexpressing a gene encoding carbonic anhydrase in recipient enterobacterium.
2. 2. The recombinant enterobacterium according to claim 1, characterized in that the cyanate transporter gene comes from Escherichia coli and the carbonic anhydrase gene comes from Escherichia coli.
3. 3. A recombinant enterobacterium according to claim 1 or 2, characterized in that: the cyanate transporter includes any of the following proteins:
4. A1) a protein with an amino acid sequence encoded by a gene encoding a cyanate transporter, said protein being presented in SEQ ID NO: 4;
5. A2) a protein obtained by substitution and/or deletion and/or addition of amino acid residues in the amino acid sequence of A1) having more than 80% identity with protein A1) and possessing cyanate transporter activity;
6. A3) a fusion protein having cyanate transporter activity obtained by attaching a label to the N-terminus and/or C-terminus of A1) or A2);
7. Carbonic anhydrase includes any of the following proteins:
8. B1) a protein with an amino acid sequence encoded by a gene encoding carbonic anhydrase, said protein being presented in SEQ ID NO: 5;
9. B2) a protein obtained by substitution and/or deletion and/or addition of amino acid residues in the amino acid sequence B1) having more than 80% identity with protein B1) and possessing carbonic anhydrase activity;
10. B3) a fusion protein possessing carbonic anhydrase activity obtained by attaching a label to the N-terminus and/or C-terminus of B1) or B2).
11. 4. A recombinant enterobacterium according to any one of paragraphs 1-3, characterized in that the coding gene for the cyanate transporter includes any of the following:
12. C1) a DNA molecule with the nucleotide sequence SEQ ID NO: 2;
13. C2) a DNA molecule characterized by more than 80% identity to the nucleotide sequence defined in C1) and encoding a cyanate transporter;
14. C3) a DNA molecule that hybridizes with the nucleotide sequence defined in any of D1)-C2) under stringent conditions and encodes a cyanate transporter;
15. The gene encoding carbonic anhydrase includes any of the following:
16. D1) a DNA molecule with the nucleotide sequence SEQ ID NO: 3;
17. D2) a DNA molecule characterized by more than 80% identity of the nucleotide sequence defined in d1) and encoding carbonic anhydrase;
18. D3) a DNA molecule that hybridizes with the nucleotide sequence defined in any of d1)-d2) under stringent conditions and encodes carbonic anhydrase.
19. 5. A method for constructing recombinant enterobacteria according to any one of paragraphs 1-4, comprising:
20. Q1) regulating the yield of arginine in microorganisms by regulating the expression of the gene encoding the cyanate transporter according to any one of paragraphs 1-4 or regulating the activity or content of the cyanate transporter, with the production of microorganisms with altered yield of arginine;