RU-2861368-C1 - STRAIN PSEUDOMONAS CITRONELLOLIS POSSESSING LIPOLYTIC ACTIVITY

RU2861368C1RU 2861368 C1RU2861368 C1RU 2861368C1RU-2861368-C1

Abstract

FIELD: biotechnology. SUBSTANCE: invention relates to a strain Pseudomonas citronellolis A13 VKM B-3953D possessing lipolytic activity. The strain is deposited in the All-Russian Collection of Microorganisms of the Federal Research Centre 'Pushchino Scientific Centre for Biological Research of the Russian Academy of Sciences', Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS) under registration number VKM B-3953D. EFFECT: production of extracellular lipolytic enzymes – lipase, esterase. 1 cl

Inventors

Ivasenko Denis Aleksandrovich
GERASIMCHUK ANNA LEONIDOVNA
Glukhova Liubov Borisovna
Levitskaia Kristina Dmitrievna
Sysoeva Anastasiia Nikolaevna

Dates

Publication Date: 20260505
Application Date: 20251218

Claims (1)

Pseudomonas citronellolis strain A13 BKM B-3953D, which has lipolytic activity.

Description

The invention relates to agricultural microbiology and biotechnology, in particular to lipolytically active microorganisms, namely to the strain Pseudomonas citronellolis A13 VKM B-3953D for the destruction of fat-containing waste. Industrial wastewater from food processing plants represents a complex, multi-component system of contaminants, among which animal and vegetable fats occupy a special place. The main sources of such wastewater include meat processing plants, dairies, oil and fat factories, fish processing plants, and large catering establishments. The operation of food processing plants and the increasing load on industrial and domestic wastewater treatment plants have made the removal of oil and fat contaminants from wastewater particularly challenging. Industrial wastewater accounts for 70-75% of the total discharge from meat processing plants. Approximately 50% of this discharge contains oil and fat contaminants (Belousova, N.I., Manuilova, T.D. Sposoby I. Means for Purifying Grease-Containing Wastewater // Meat Industry. 2007. No. 7, pp. 57-60). Removing fat deposits from the sewer systems of food processing plants and disposing of them in grease traps is a serious problem. There are many wastewater treatment methods, including pneumatic, hydrodynamic, mechanical, thermal, and chemical cleaning methods. Ultrasonic cleaning effectively removes stubborn contaminants using ultrasonic vibrations. Grease traps, a device for mechanical wastewater treatment, can be installed on pipes. Special equipment that removes blockages using pressure—so-called cleaning machines—is also used, but this method requires frequent repetition, depending on the load on the sewer system. Recently, bioremediation methods based on the use of microorganisms that degrade oil and fat waste have been increasingly used to address this problem. The use of biopreparations in the sewer system helps remove existing fatty deposits and slow the formation of new ones by creating an active biofilm. When exposed to wastewater, this biofilm breaks down complex fat molecules into simple, safe substances. A known method for purifying fat-containing wastewater involves introducing a culture of microscopic fungi into the wastewater. The fungi used are Aspergillus niger 288, while the pH and temperature of the wastewater are maintained within the ranges of 4.5-5 and 28-30°C, respectively, and the water is treated for 48-50 hours (RU Patent for Invention 2016851, IPC C02F 3/34; published: 30.07.1994). The method improves the extraction of polluting components from wastewater and increases the growth of microbial biomass, with subsequent use as livestock feed. The microbiological preparation "BionexGT" (https://bionex.pro/production/industrial/septic-treat?ysclid=mj41mkwsbj486627544, accessed: 12/11/2025) contains 8 strains of facultative anaerobic microorganisms that promote the breakdown of fat macromolecules into simpler substances, eliminate pathogenic microflora and reduce the level of pollution in industrial wastewater, and also prevent the formation of fatty deposits on the surfaces of separation systems and pumping equipment. A known method for purifying wastewater containing residues of edible oils and fats is characterized in that a fermentation process is carried out on wastewater using a powdered biopreparation created on the basis of bacterial cultures Acinetobacter species M-2, Acinetobacter species M-3, Arthrobacter species M-4, Rhodococcus species M-5, in the presence of sources of nitrogen, phosphorus, potassium and microelements at a temperature of 10-50 ° C. (patent for invention of the Russian Federation 2161595, IPC C02F 3/34; C12N 1/20; published: 10.01.2001). The method is characterized by the following features: the following features: the use of biopreparations in the form of dry powders and environmental friendliness due to the use of non-pathogenic bacterial strains for wastewater treatment. The strain Pseudomonas extremaustralis ARC 38 VKPM B-13084 is known for the destruction of oil and oil products in an aquatic environment at temperatures from +20 to -2.5°C and a salinity of 30±10 g/l under nutrient-poor conditions (patent for invention of the Russian Federation No. 2694610, IPC C12N 1/20, C02F 3/34; published: 16.07.2019). A microbial preparation is known for the disposal of hydrocarbon contaminants in the aquatic environment (patent for invention of the Russian Federation No. 2697381, IPC C02F 3/34, C12N 1/26; published: 13.08.2019) at a temperature of +20 to -2.5 ° C and a salinity of 30 ± 10 g / l, containing a biomass of psychoactive microorganisms: strain Psychrobacter cibarius ARC 35 VKPM B-13081, strain Pseudomonas extremaustralis ARC 38 VKPM B-13084, strain Yarrowia lipolytica ARC 48 VKPM Y-4347 in the form of a suspension or dried powder, taken in a ratio of 1: 1: 1 by volume or by weight, respectively, with an acceptable deviation from the specified value of no more than 10% with concentration of micr