RU-2861439-C1 - METHOD FOR REARING COCCINELLID LEIS DIMIDIATA FABR.

RU2861439C1RU 2861439 C1RU2861439 C1RU 2861439C1RU-2861439-C1

Abstract

FIELD: biotechnology. SUBSTANCE: invention is a method for rearing the coccinellid Leis dimidiata Fabr., comprising rearing larvae on a nutrient medium containing soy flour, dry brewer's yeast, methylparaben, agar-agar and distilled water, characterised in that using a nutrient medium additionally containing fresh grain aphids and Nutrilon milk formula in the following content of initial components, mas.%: soy flour 10, fresh grain aphids 10, dry brewer's yeast 2, Nutrilon milk formula 14.5, methylparaben 0.12, agar-agar 2, distilled water the balance. EFFECT: significant reduction in the consumption of expensive, labour-intensive and energy-intensive components to manufacture. 1 cl

Inventors

Kashutina Evgeniia Viktorovna
Bugaeva Liudmila Nikolaevna
Ignateva Tatiana Nikolaevna
Iasiuk Larisa Viktorovna
Zhugareva Natalia Anatolevna

Dates

Publication Date: 20260505
Application Date: 20250521

Claims (2)

A method for breeding the coccinellid Leis dimidiata Fabr., which involves growing the larvae on a nutrient medium containing soy flour, dry brewer's yeast, metaben, agar-agar and distilled water, characterized in that the nutrient medium is used, additionally containing fresh cereal aphids and Nutrilon milk mixture with the following content of the initial components, wt. %:
Soy flour 10 Fresh cereal aphids 10 Dry brewer's yeast 2 Nutrilon milk formula 14.5 Metaben 0.12 Agar-agar 2 Distilled water rest

Description

The invention relates to biotechnology, in particular to methods of breeding beneficial insects. A method for breeding the coccinellid Harmonia axyridis Hall is known, which involves growing coccinellid larvae on a nutrient medium containing casein, sucrose, cystine, dry milk, dry bean aphid, corn and coconut oil, Wesson's salt, cholesterol, brewer's yeast autolysate, tocopherol, choline chloride, ascorbic, folic, para-aminobenzoic, nicotinic, glutamic acids, pyridoxine, biotin, calcium pantothenate, arginine, methionine, agar-agar, distilled water (Author's certificate No. 801827, A01K 67/00, 1980). The disadvantage of this method is the high consumption of expensive and labor-intensive components of the nutrient medium. The closest to the proposed method is the method for breeding the coccinellid Harmonia axyridis Hall., which involves growing larvae on a nutrient medium containing soy flour, sucrose, Wesson's salt, dry milk, melted agar-agar, palm kernel oil, dry brewer's yeast, dry cereal aphid, inositol, ascorbic acid, tocopherol, vitamins B1, B6, B12, metaben and distilled water (RU Patent No. 2520860, C12N 1/00, A01K 67/033, published on 27.06.2014). Another disadvantage of this method is the high consumption of expensive, labor-intensive and energy-consuming components to manufacture. The technical result of the invention is a reduction in the consumption of expensive, labor-intensive and energy-consuming components. This result is achieved by the fact that the proposed method for breeding the coccinellid Leis dimidiata Fabr. involves growing adults and larvae on a nutrient medium containing soy flour, fresh cereal aphids, dry brewer's yeast, Nutrilon milk mixture, metaben, agar-agar and distilled water in the following ratio of components, wt. %: Soy flour10Fresh cereal aphids10Dry brewer's yeast2Nutrilon milk formula14.5Metaben0.12Agar-agar2Distilled waterthe rest The method of breeding the coccinellid Leis dimidiata Fabr. is carried out as follows. An artificial nutrient medium is prepared by dosing and mixing the components in the specified ratio. The medium is poured into Petri dishes and stored in the refrigerator at 4-6°C. The medium has a gelatinous consistency and is easy to use. Larvae are reared in universal cages at a temperature of 23-26°C on the prepared nutrient medium applied to pieces of sterile polyethylene film. The feed is changed daily. To rear 1,000 Leis dimidiata Fabr. individuals, 750 g of nutrient medium is required. A comparative analysis of the composition of the nutrient media used in the proposed and closest analogues revealed that the number of components is significantly reduced when culturing Leis dimidiata Fabr. adults and larvae on the nutrient medium according to the invention. Components such as sucrose, palm kernel oil, Wesson's salt, tocopherol, ascorbic acid, vitamins B1, B6, B12, inositol, and dry milk are excluded. These components are replaced by Nutrilon milk formula, which does not affect the quality of the medium, as it contains a balanced amount of all the elements necessary for insect life. Fresh cereal aphids are used instead of the labor-intensive and energy-consuming component of dried cereal aphids. Experiments have shown that changing the nutrient medium formulation according to the invention does not degrade the conditions or results of culturing the coccinellid Leis dimidiata Fabr. The beetles actively fed for 60 days. When transferred to natural food, adults mated and laid eggs on days 4-5. Average daily fecundity was 21.7%. Viable larvae emerged from the resulting eggs within 5-6 days. Preimaginal stage survival was 85.3%, which is consistent with the closest analogue (RU 2515688 - 85% survival, egg laying within 8-10 days, average daily fecundity of 19.1%). With subsequent maintenance of emerged beetles on the proposed nutrient medium, the lifespan of adults is over three months. Thus, the proposed method for breeding the coccinellid Leis dimidiata Fabr. is cheaper and more technologically advanced, which allows its wide application in the practice of mass breeding of entomophages for the organization of biological plant protection.