RU-2861488-C1 - METHOD FOR PRODUCING ANTIMICROBIAL WOUND DRESSING WITH SELF-STERILISING SURFACE BASED ON BICOMPONENT NANOPARTICLES

Abstract

FIELD: materials science; nanotechnology in medicine. SUBSTANCE: invention can be used for the treatment of chemical, thermal and physical damage to the skin, including burns, cuts and eczema of various etiologies. A method for producing an antimicrobial wound dressing with a self-sterilising surface includes disinfecting ZnO-Ag nanoparticle powder at 100±1 °C for 2±0.1 h, preparing a 1% aqueous suspension of said nanoparticles in distilled water, dispersing the suspension by ultrasonic treatment for 15±1 min, and impregnating a fibrous material based on cellulose acetate by immersing it in said suspension for at least 60 s, followed by removing excess liquid and air drying at room temperature. Wherein, using ZnO-Ag nanoparticles with an average size of 76±2 nm and a component ratio in the particle ZnO:Ag - 72:28 at.%, having a heterophase "Janus"-type structure with a hexagonal wurtzite zinc oxide phase and a face-centred cubic metallic silver phase, obtained by joint electrical explosion of a twist of zinc and silver wires in an oxygen-containing atmosphere. EFFECT: obtaining a wound dressing with improved antimicrobial properties and reliable attachment of particles to cellulose fibres, which imparts wash resistance and the possibility of reusable use. 5 cl, 8 dwg, 2 tbl, 10 ex

Inventors

• Ivanova Liudmila Iurevna
• BAKINA OLGA VLADIMIROVNA
• Chzhou Valeriia Romanovna
• Iafaeva Anastasiia Eduardovna
• Lerner Marat Izrailevich
• VOROZHTSOV ALEKSANDR BORISOVICH

Dates

Publication Date

20260505

Application Date

20251203

Claims (5)

1. 1. A method for producing an antimicrobial dressing material with a self-sterilizing surface based on bicomponent nanoparticles, which involves disinfecting ZnO-Ag nanoparticle powder in a drying oven at 100±1 °C for 2±0.1 h, preparing a 1% aqueous suspension of said nanoparticles in distilled water, dispersing the suspension by ultrasonic treatment for 15±1 min for deagglomeration, impregnating a fibrous material based on cellulose acetate by immersion in said suspension for at least 60 s, removing excess liquid and drying the material in air at room temperature to constant weight, using ZnO-Ag nanoparticles with an average size of 76±2 nm and a component ratio in the ZnO:Ag particle of 72:28 at.%, having a Janus-type heterophase structure with an oxide phase zinc with a hexagonal wurtzite structure and a metallic silver phase with a face-centered cubic lattice, obtained by a joint electrical explosion of twisted zinc and silver wires of different diameters in an oxygen-containing atmosphere.
2. 2. The method according to claim 1, wherein the ζ-potential of the nanoparticles is positive and is 34±3 mV at pH 7.0-7.5, which ensures the stability of aqueous suspensions of the nanoparticles.
3. 3. The method according to claim 1, in which the joint electrical explosion of twisted zinc and silver wires of a given diameter is carried out in a mixture of 80 vol.% Ar and 20 vol.% O 2 , which provides the selected ratio of zinc oxide and silver in the particle, according to the results of electron microscopy.
4. 4. The method according to claim 1, in which the synthesis of particles is carried out by the method of electrical explosion of wires, due to the features of the synthesis method, the ZnO-Ag nanoparticles do not contain impurities in their phase composition, which ensures the purity of the phase boundary in the heterophase particle, according to X-ray phase analysis.
5. 5. The method according to claim 1, in which, before impregnating the fibrous material, the suspension is additionally treated with ultrasound, ensuring that the proportion of particles with a hydrodynamic diameter of ≤100 nm is at least 75%, based on the results of disk centrifugal sedimentation.

Description

The invention relates to the field of materials science and nanotechnology, as applied to medicine, namely to antimicrobial personal protective equipment and methods for producing them from polymer fibers and bicomponent nanoparticles, for the treatment of chemical, thermal and physical damage to the skin, including burns, cuts and eczema of various etiologies. There are various known methods for modifying dressing materials to impart additional functions, such as absorbing pathogenic secretions, heat-dissipating, antimicrobial, self-sterilizing, etc. For example, in a patent (patent RU 2199350 C1, A61L 15/00, A61K 33/44, A61P 17/02, C01B 31/04 published 27.02.2003) a carbon mixture of graphite and carbon nanocrystals was used as a sorption material to remove excess moisture from the wound surface. The patent (RU 2602360 C1, A61F 13/022, published November 20, 2016) describes dressings comprising a metal substrate, such as aluminum, having a burn-facing side for direct contact with the burn to transfer heat away from the burn by thermal conductivity, and a heat-absorbing side opposite the burn-facing side for contact with a hydrogel to transfer heat away from the metal layer by thermal conductivity. The thin aluminum layer and the hydrogel heat absorber bonded to it provide flexibility and effective heat transfer properties for rapid cooling of the burn wound. A patent (RU 2669626 C2, D06M 11/83, published October 12, 2018) proposes a method for producing cellulose fibers impregnated with metal nanoparticles, in particular silver nanoparticles. The method involves swelling the cellulose fibers in an aqueous alkaline solution. The swollen cellulose fibers are removed from the aqueous alkaline solution and mixed with an aqueous solution of a silver salt and a polymer solution such that the fibers become impregnated with metal nanoparticles. The swollen cellulose fibers impregnated with the metal nanoparticles are then removed from the solution. The invention enables the creation of cellulose fibers for the production of dressings with excellent antimicrobial properties. The patent (RU 2314834 C1, A61L 15/18, A61F 13/00, A61L 15/44, A61P 17/02, published 20.01.2008) describes a wound dressing based on woven and non-woven materials of natural or synthetic origin, containing particles of a metal that has biological activity against pathogenic flora, characterized in that as metal particles it contains nanoparticles of silver from 80 to 99.9%, iron from 0.1 to 20%, aluminum from 0.1 to 20%, copper from 0.1 to 20%, which are applied in a vacuum chamber using magnetron sputtering. The size of the nanoparticles does not exceed 0.1 μm. Metal nanoparticles can be applied to both sides of the coating. The presence of nanoparticles ensures higher activity of metals, and the method of applying metal particles (magnetron sputtering) ensures the sterility of the coating. The widespread use of silver nanoparticles as antimicrobial agents has enabled the study of these particles not only for their biocidal properties but also for their safety. Over the past decade, numerous review papers on the cytotoxicity of silver nanoparticles have been published, including the most recent (Jaswal T., Gupta J., "A review on the toxicity of silver nanoparticles on human health" // Materials Today: Proceedings. - 2023. - Vol. 81. - Pp. 859-863). This limits the use of silver particles as the primary biocidal component. In this regard, the potential of bicomponent metal or oxide nanoparticles as antimicrobial agents is being considered. In the study (Ranathunga K. et al. Preparation and characterization of Fe-ZnO cellulose-based nanofiber mats with self-sterilizing photocatalytic activity to enhance antibacterial applications under visible light //RSC advances. - 2024. - V. 14. - No. 26. - P. 18536-18552), a composite of cellulose-based nanofibers and Fe-ZnO nanoparticles with self-sterilizing photocatalytic activity was developed and studied to improve antibacterial action under the influence of visible light. Using the coprecipitation procedure, Fe-ZnO nanohybrids were obtained with different ZnO concentrations (0, 3, 5, 7, and 10%) and incorporated into a cellulose acetate-based polymer matrix by electrospinning. An IC50 value of 81.44 μg/mL for the particles was demonstrated. Disk diffusion analysis confirmed the antimicrobial activity of the membrane with a nanohybrid containing 5% ZnO obtained by electrospinning against strains (ATCC 25923), (ATCC 49619), (ATCC 25922), (ATCC 27853), and (ATCC 10231) in visible light. The disadvantages of the method include high concentrations of the active substance to achieve the antimicrobial effect. The electrospinning method requires additional equipment, time, and leads to additional costs for reagents. In addition, the studies showed that the minimum inhibitory concentration of Fe-ZnO nanoparticles was 162.88 μg/ml, which is not the best indicator compared to the activity of nanoparticles of oth