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RU-2861545-C1 - METHOD FOR ASSESSING BIOLOGICAL AGE

RU2861545C1RU 2861545 C1RU2861545 C1RU 2861545C1RU-2861545-C1

Abstract

FIELD: medicine. SUBSTANCE: invention relates to clinical laboratory diagnostics, gerontology and dentistry, and can be used to determine biological age. Receiving the patient's oral fluid. In the oral fluid, determining the following parameters: total protein in g/l, albumin in g/l, alkaline phosphatase in U/l, inorganic phosphorus in mmol/l and total calcium in mmol/l, determining the phosphorus-calcium ratio by dividing the concentration of inorganic phosphorus by the concentration of total calcium, creatinine in mcmol/l, urea in mmol/l, cholesterol in mmol/l, creatine kinase MB in U/l, beta-galactosidase in ng/ml, final glycation products in ng/ml. For each obtained parameter, determining the value of the logarithm to the base two. Calculating the biological age using the formulas: first, the logarithmised value of the biological age, then the biological age of the person. EFFECT: ability to accurately determine the biological age and assess the rate of human aging, and can be used in assessing the effectiveness of therapeutic and preventive measures, especially in dentistry, by determining the claimed laboratory parameters in the patient's oral fluid. 1 cl, 1 tbl, 3 ex

Inventors

  • BAZARNYJ VLADIMIR VIKTOROVICH
  • Kopenkin Maksim Aleksandrovich

Dates

Publication Date
20260505
Application Date
20250428

Claims (16)

  1. A method for determining biological age, including obtaining oral fluid from a patient, characterized by the fact that the following indicators are determined in the oral fluid: total protein in g/l, albumin in g/l, alkaline phosphatase in U/l, inorganic phosphorus in mmol/l and total calcium in mmol/l, with the determination of the phosphorus-calcium ratio by dividing the concentration of inorganic phosphorus by the concentration of total calcium, creatinine in μmol/l, urea in mmol/l, cholesterol in mmol/l, creatine kinase MB in U/l, beta-galactosidase in ng/ml, advanced glycation end products in ng/ml, for each obtained indicator the logarithm value to the base two is determined, from which the logarithmic value of biological age BV log is then determined using the formula
  2. where BV log is the logarithmic value of biological age with an accuracy of three decimal places,
  3. logarithmized values to base two:
  4. OB - total protein,
  5. ALB - albumin,
  6. ALP - alkaline phosphatase,
  7. PCa - phosphorus-calcium ratio,
  8. Kr - creatinine,
  9. Mo - urea,
  10. HS - cholesterol,
  11. CCMB - creatine kinase MB,
  12. BG - beta-galactosidase,
  13. APG - advanced glycation end products;
  14. biological age is determined by the formula
  15. ,
  16. where BA is biological age, in years.

Description

The invention relates to medicine, specifically to clinical laboratory diagnostics, gerontology, and dentistry, for determining a person's biological age based on laboratory analysis of oral fluid. The method can be used to assess the rate of aging and the effectiveness of treatments. One of the modern demographic trends is the gradual increase in the proportion of elderly and senile individuals in the population. Aging-related morphofunctional changes affect the entire body, including the tissues and organs that form the oral cavity. Dental hard tissues are subject to enamel thinning and wear, periodontal disease is characterized by gum recession, thinning of the periodontal ligament, and a shortening of the dental arch, a decrease in the number of elastic fibers in the oral mucosa, depletion of microcirculation, and the development of salivary gland hypofunction. Dental health largely determines quality of life in old age. Passport age does not always reflect the progression of the aging process. Cases of delayed or accelerated aging are often observed, when biological age is less than or greater than passport age. Various methods for assessing biological age have been proposed, based on metabolic, functional, and morphological parameters, using various mathematical models. Methods for determining biological age in men and women have been proposed, based on the determination of clinical and functional indicators with subsequent calculation of "derived indicators" [1, 2]. In men, hearing acuity, accommodation, vital capacity, arterial systolic, diastolic and pulse pressure, statistical balancing on the left leg, the Wechsler symbol-digital test for attention, body weight, subjective health assessment, and the electrocardiographic Q-T interval are determined. In women, in addition to the above, breath holding on exhalation is determined, the R-R interval is assessed instead of the Q-T interval, and the Wechsler attention test is not performed. Biological age is estimated using multiple linear regression equations. Although the proposed methods are highly accurate—the R2 determination coefficient for men was 0.87 and for women 0.86—they are labor-intensive and time-consuming to implement. This method requires the use of a tonometer, an open-type spirograph, medical scales, Sivtsev charts, an audiometer, an electrocardiograph, and other equipment. Determining biological age using this method requires training for both the specialists conducting the functional tests and the subject. A method for determining biological age based on cardiac ultrasound data exists [3]. The ratio of the peak velocity of early diastolic filling of the left ventricle to the peak velocity of late diastolic filling of the left ventricle (E/A) and the end-systolic myocardial stress (ESMS) index are determined. Biological age is estimated using a formula proposed by the authors and compared with the calculated value of the "proper" biological age to assess the rate of aging. The method is based on cardiac ultrasound, making it complex and labor-intensive. The resulting model for determining bioage is not highly accurate, with the R2 determination coefficient being only 47.5%. Furthermore, the authors compare the obtained bioage with a calculated "expected" bioage, rather than with the chronological age, making it difficult to evaluate. The closest analogue (prototype) is the method of assessing biological age based on hemostasis parameters [4]. This method is based on determining the level of fibrinogen in blood plasma, the number of platelets, assessing the activated partial thromboplastin time, fibrinolytic activity, the concentration of soluble fibrin monomer complexes and the level of antithrombin 3. Biological age is determined using multiple linear regression and discriminant analysis. The method is invasive due to the use of blood plasma. Obtaining biomaterial requires personnel trained in phlebotomy techniques and maintaining sterile conditions during blood collection. Some of the proposed laboratory methods are relatively complex or outdated, making it impossible to replicate in any accessible clinical diagnostic laboratory. The method requires complex, expensive equipment, personnel specially trained in hemostasis, and costs not only for reagents but also for blood collection equipment. The aim of the claimed invention is to develop a simple to implement and cost-effective method for determining the biological age of a person, which is based on a laboratory analysis of oral fluid (OF), which is simple, non-invasive and does not require special training and participation of the person being examined [5]. The technical result of the claimed method consists in increasing the availability of biological age assessment due to its simplification and cost-effectiveness with high accuracy. The proposed method for assessing biological age is carried out by laboratory measurement of a number of parameters of oral fluid: total protein; al