US-12616105-B2 - Method for producing mycelial sheet

US12616105B2US 12616105 B2US12616105 B2US 12616105B2US-12616105-B2

Abstract

A method for producing a mycelial sheet includes: subjecting a fungus to static cultivation in a liquid medium containing a cellulose, so as to allow the fungus to form the mycelial sheet on a surface of the liquid medium; and collecting the mycelial sheet from the surface of the liquid medium containing the cellulose.

Inventors

Hsiao-Ping KUO
Yi-Tzu Kuo
Shyue-Tsong HUANG
Han-Yun Li
Wan-Rou Lin
Sung-Yuan Hsieh

Assignees

FOOD INDUSTRY RESEARCH AND DEVELOPMENT INSTITUTE

Dates

Publication Date: 20260505
Application Date: 20241204
Priority Date: 20240617

Claims (10)

1 . A method for producing a mycelial sheet, comprising: subjecting a fungus to static cultivation in a liquid medium containing a cellulose having a particle size ranging from 0.035 mm to 4 mm, so as to allow the fungus to form the mycelial sheet on a surface of the liquid medium; collecting the mycelial sheet from the surface of the liquid medium containing the cellulose; and subjecting the mycelial sheet collected from the surface of the liquid medium containing the cellulose to a washing treatment so as to remove the cellulose, wherein the mycelial sheet is free of the cellulose and the static cultivation is the only cultivation performed in the method and the static cultivation is performed under a stationary condition without stirring or shaking.
2 . The method as claimed in claim 1 , wherein the cellulose is present in an amount ranging from 0.05 wt % to 0.5 wt % based on 100 wt % of the liquid medium.
3 . The method as claimed in claim 1 , wherein the cellulose is selected from the group consisting of a bacterial cellulose, a plant cellulose, and a combination thereof.
4 . The method as claimed in claim 3 , wherein the plant cellulose is selected from the group consisting of a potato cellulose, a wheat cellulose, an oat cellulose, and combinations thereof.
5 . The method as claimed in claim 3 , wherein when the liquid medium contains the bacterial cellulose, the mycelial sheet thus obtained is free from the bacterial cellulose.
6 . The method as claimed in claim 3 , wherein when the liquid medium contains the plant cellulose, the mycelial sheet thus obtained is free from the plant cellulose.
7 . The method as claimed in claim 3 , wherein when the liquid medium contains the combination of the bacterial cellulose and the plant cellulose, the mycelial sheet thus obtained is free from the combination of the bacterial cellulose and the plant cellulose.
8 . The method as claimed in claim 1 , wherein the fungus is selected from the group consisting of Cordyceps spp., Trametes spp., Ganoderma multipileum, Inonotus tabacinus, Irpex brevis, Pleurotus cornucopiae, Schizophyllum commune, Terana coerulea , and combinations thereof.
9 . The method as claimed in claim 8 , wherein the Cordyceps spp. is selected from the group consisting of Cordyceps cateniobliqua, Cordyceps farinosa, Cordyceps militaris, Cordyceps tenuipes , and combinations thereof.
10 . The method as claimed in claim 8 , wherein the Trametes spp. is selected from the group consisting of Trametes hirsuta, Trametes gibbosa, Trametes versicolor , and combinations thereof.

Description

CROSS-REFERENCE TO RELATED APPLICATION This application claims priority to Taiwanese Invention patent application No. 113122371, filed on Jun. 17, 2024, the entire disclosure of which is incorporated by reference herein. FIELD The disclosure relates to a method for producing a mycelial sheet. BACKGROUND A mycelial sheet, also known as mycelial mat, is a sheet material generated from a fungus. The mycelial sheet has been used as a substitute for leather or cloth due to good mechanical property (generally with a tensile strength of approximately 1 MPa to 5 MPa) thereof. The mycelial sheet is mainly composed of mycelia, and some studies have found that adding a cellulose to the mycelial sheet to form a mycelium-cellulose composite material can further improve the mycelial sheet's mechanical property. Currently, one of two commonly used methods for producing the mycelial sheet may include subjecting the fungus to solid-state cultivation, so as to allow the fungus to form the mycelial sheet, followed by collecting the mycelial sheet on a surface of a solid culture medium; another one of the methods may include subjecting the fungus to shaking cultivation in a liquid medium, so as to obtain a resultant liquid culture, followed by subjecting the resultant liquid culture to filtration (in some cases, further subjected to pressing after the filtration), thereby obtaining the mycelial sheet. However, with respect to the aforesaid two methods, the former is more time-consuming, and the latter is more complicated in operation. SUMMARY Therefore, an object of the disclosure is to provide a method for producing a mycelial sheet, which can alleviate at least one of the drawbacks of the prior art. The method includes: subjecting a fungus to static cultivation in a liquid medium containing a cellulose, so as to allow the fungus to form the mycelial sheet on a surface of the liquid medium; andcollecting the mycelial sheet from the surface of the liquid medium containing the cellulose. BRIEF DESCRIPTION OF THE DRAWINGS Other features and advantages of the disclosure will become apparent in the following detailed description of the embodiment(s) with reference to the accompanying drawings. It is noted that various features may not be drawn to scale. The FIGURE shows a dry weight of a mycelial sheet determined in each group of each fungal strain of Example 1, infra. DETAILED DESCRIPTION It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Taiwan or any other country. For the purpose of this specification, it will be clearly understood that the word “comprising” means “including but not limited to”, and that the word “comprises” has a corresponding meaning. Unless otherwise defined, all technical and scientific terms used herein have the meaning commonly understood by a person skilled in the art to which the present disclosure belongs. One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present disclosure. Indeed, the present disclosure is in no way limited to the methods and materials described. By conducting research, the applicant surprisingly found that by subjecting a fungus to static cultivation in a liquid medium containing a cellulose, a mycelial sheet can be formed on a surface of the liquid medium, which not only makes forming of the mycelial sheet quick and easy, but also allows the mycelial sheet to be completely formed and to have a good tensile strength. Therefore, the present disclosure provides a method for producing a mycelial sheet, which includes: subjecting a fungus to static cultivation in a liquid medium containing a cellulose, so as to allow the fungus to form the mycelial sheet on a surface of the liquid medium; andcollecting the mycelial sheet from the surface of the liquid medium containing the cellulose. As used herein, the terms “mycelial sheet,” “mycelial mat,” “fungal sheet” and “fungal mat” can be interchangeably used, and mean a continuous flat sheet formed by interconnection of fungal hyphae. As used herein, the term “fungus” is intended to cover any fungal species that is capable of producing a mycelium (i.e., a mycelium-producing fungus or a filamentous fungus). Examples of the fungal species may include, but are not limited to, Cordyceps spp., Trametes spp., Ganoderma multipileum, Inonotus tabacinus, Irpex brevis, Pleurotus cornucopiae, Schizophyllum commune, Terana coerulea, and combinations thereof. In certain embodiments, the Cordyceps spp. may be selected from the group consisting of Cordyceps cateniobliqua, Cordyceps farinosa, Cordyceps militaris, Cordyceps tenuipes, and combinations thereof. In some embodiments, the Cordyceps farinosa may be Cordyceps farinosa deposited at the Bioresource Collection and Research Center (BCRC) unde