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US-12616109-B2 - Spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium

US12616109B2US 12616109 B2US12616109 B2US 12616109B2US-12616109-B2

Abstract

Provided herein are spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium , and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Also provided herein are genomic fragments providing the present resistances and use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium , and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Provided herein are a spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium : including a genomic fragment of Spinacia tetrandra located on chromosome 4 between positions 8255074 and 8620598 of the spinach reference genome, the genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance and including a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance.

Inventors

  • Jordi Cornelis Boshoven
  • Stefanus Johannes Kaandorp
  • Raimon Jozef Laan
  • Roelof Marinus Veenstra
  • Albertus Johannes Maria Schrijver

Assignees

  • BEJO ZADEN B.V.

Dates

Publication Date
20260505
Application Date
20201124

Claims (9)

  1. 1 . A spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium, comprising a genomic fragment of Spinacia tetrandra located on chromosome 4 of said spinach plant and between positions 8255074 and 8620598 of a spinach reference genome, said genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance; comprising a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance; and comprising a genomic fragment located on chromosome 3 and between positions 1201575 and 1220905 of the spinach reference genome providing Cucumber Mosaic Virus (CMV) resistance, wherein said genomic fragment of Spinacia tetrandra providing Peronospora farinosa resistance, said genomic fragment providing Stemphylium vesicarium resistance, and said genomic fragment providing Cucumber Mosaic Virus (CMV) resistance are obtained from a spinach plant deposited under deposit number NCIMB 43676.
  2. 2 . The spinach plant according to claim 1 comprising in its genome one or more nucleotide sequences selected from the group consisting of SEQ ID Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 and 21.
  3. 3 . The spinach plant according to claim 1 , comprising in its genome one or more nucleotide sequences selected from the group consisting of SEQ ID Nos. 31, 33, 35, 37, 39, 41, and 43.
  4. 4 . The spinach plant according to claim 1 , comprising in its genome SEQ ID Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 31, 33, 35, 37, 39, 41, and 43.
  5. 5 . The spinach plant according claim 1 , comprising in its genome one or more nucleotide sequences selected from the group consisting of SEQ ID Nos. 23, 25, 27, and 29.
  6. 6 . The spinach plant according to claim 1 , comprising in its genome SEQ ID Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43.
  7. 7 . The spinach plant according to claim 1 , wherein said Peronospora farinosa resistance comprises resistance to at least Peronospora farinosa isolates 1 to 17, UA2020-01E, and SP1924.
  8. 8 . A plant cell, plant tissue, plant part or seed of a spinach plant according to claim 1 ; wherein the plant cell, plant tissue, plant part or seed each comprises: said genomic fragment of Spinacia tetrandra providing Peronospora farinose resistance, said genomic fragment providing Stemphylium vesicarium resistance, and said genomic fragment providing Cucumber Mosaic Virus (CMV) resistance obtained from a spinach plant deposited under deposit number NCIMB 43676.
  9. 9 . A method of providing a spinach plant resistant to Peronospora farinosa and/or Stemphylium vesicarium , comprising crossing a spinach plant that is susceptible to Peronospora farinosa and/or Stemphylium vesicarium with the spinach plant according to claim 1 and selecting offspring that are resistant to Peronospora farinosa and/or Stemphylium vesicarium.

Description

CROSS REFERENCE TO RELATED APPLICATION This application is the United States national phase of International Patent Application Number PCT/EP2020/083246, filed Nov. 24, 2020, the disclosure of which is hereby incorporated by reference in its entirety. The Sequence Listing associated with this application is filed in electronic format via EFS-Web and is hereby incorporated by reference into the specification in its entirety. The name of the text file containing the Sequence Listing is 2303536 ST25.txt. The size of the text file is 17,351 bytes, and the text file was created on May 3, 2023. BACKGROUND OF THE INVENTION Field of the Invention The present invention relates to spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium, and to spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). The present invention also relates to genomic fragments providing the present resistances and to the use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Description of Related Art Spinach is commercially grown worldwide for its attractive and nutritious leaves. In 2018, production of spinach was close to 26 million tons worldwide. Spinach (Spinacia oleracea) is a member of the Amaranthaceae family, subfamily Chenopodioideae. Other well-known family members include quinoa and beet. The latter is a cultivated plant of major importance for agriculture with sugar beet, red beet and Swiss chard as examples. Regarding nutritional value, while providing only a small amount of calories (only 23 for 100 grams of cooked spinach), spinach is a rich source of vitamins A, B2 (or folate), B6, C, E and K; magnesium, manganese, calcium, potassium, iron and dietary fibre. Spinach flowering is induced by (long) day length and under optimal conditions can reach even up to 4 generations in a year with a life cycle from seed to new harvest completed within 3 months. A bottleneck can be caused by seed dormancy. Spinach is a wind pollinator and its pollen can reach far. A line is considered male if it converts from female or mixed flowering to (all) male flowering within a week. Female lines stay so for at least three weeks without producing any pollen. Hybrids of spinach can be produced making use of plants which have a female flowering phase and plants which have a male flowering phase as pollinator. Before the female plants develop male flowers, all female flowers are fertilized by the male plant. The setting of seeds occurs rapidly within 3 days and after that the ripening of the seed takes approximately a month. Under optimal conditions commercial elite spinach lines are grown and harvested within days to obtain baby leaf spinach. Breeding resulted in spinach plants which are rapid growing without premature flowering. Older varieties tend to have narrower leaves and have a stronger, somewhat bitter taste; newer varieties have broader leaves and a milder taste. Also, recent types have little tendency for bolting in warm conditions and therefore will not prematurely flower and produce seeds. Spinach is cultivated for the leaves. Commercial spinach can have round leaves of dark green color. The leaf morphology is of interest to spinach breeders. A significant share of the market of cultivated spinach is the early harvested baby leaf spinach. For spinach growers it is important that the leaves stand straight up which facilitates easy harvest, dark green colour is desired. Spinach originates from middle Asia but it is now produced all over the world. Traditional areas where spinach was grown as a crop are Europe and Northern America, however contemporarily the biggest volume of spinach is produced in China. Spinach is produced for the food processing industry (canned or frozen spinach) as well as for the fresh market, where especially baby leaf spinach is in demand Breeders develop lines with characteristics best suited for the location or the purpose. An important development in the production and sales of fresh spinach was the introduction of bagged spinach. For this application the desired leaf morphology is such that the leaves are not too closely packed together that are found in the partly savoyed types. Basic types of spinach are on the market: A savoy type with dark green, curly and crinkly leaves (for the fresh market);A flat, or smooth, leaf spinach with broad, smooth leaves that can be cleaned easily. This type is used for industry (canned or frozen spinach, as well as processed food and baby food;Semi savoy is an intermediate type of spinach with a comparable texture as the savoy type but easy to clean as the smooth type of spinach. It is cultivated both for fresh market and industry.An oriental type which is heat tolerant, has long petioles, pointed leaves with several side lobes and as plant has an upright growth. Most spinach is produced at high plant densities for fresh market p