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US-12616112-B2 - Canola inbred 4PWGT51R

US12616112B2US 12616112 B2US12616112 B2US 12616112B2US-12616112-B2

Abstract

A novel canola variety designated 4PWGT51R and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety 4PWGT51R with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 4PWGT51R through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety 4PWGT51R or a locus conversion of 4PWGT51R with another canola variety.

Inventors

  • Chadwick Bruce Koscielny

Assignees

  • PIONEER HI-BRED INTERNATIONAL, INC.

Dates

Publication Date
20260505
Application Date
20231031

Claims (20)

  1. 1 . A seed, plant, plant part or plant cell of canola line 4PWGT51R, representative seed of the canola line 4PWGT51R having been deposited under NCMA accession number 202309004.
  2. 2 . The seed, plant, plant part, or plant cell of claim 1 , wherein the seed, plant, plant part, or plant cell is a plant part and wherein the plant part is an ovule or pollen.
  3. 3 . An F1 hybrid Brassica seed of 4PWGT51R produced from the cross of the plant or plant part of claim 1 with a different Brassica plant.
  4. 4 . An F1 hybrid Brassica plant or a plant part thereof produced by growing the F1 hybrid seed of claim 3 , wherein the plant part comprises at least one F1 hybrid plant cell.
  5. 5 . A method for producing a second Brassica plant, the method comprising applying plant breeding techniques to the F1 plant or plant part of claim 4 to produce the second Brassica plant.
  6. 6 . A method for producing progeny Brassica plants, the method comprising doubling the chromosomes of haploid plants produced from haploid embryos produced by microspore culture from the plant or plant part of claim 4 .
  7. 7 . A method of producing a modified seed, plant, plant part, or plant cell, the method comprising genome editing the seed, plant, plant part, or plant cell of claim 1 , thereby producing the modified seed, plant, plant part, or plant cell.
  8. 8 . A method for producing a second canola plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 1 .
  9. 9 . A method of producing a modified progeny seed, the method comprising: a) crossing or selfing the plant or plant part of claim 1 to produce a progeny seed and genome editing the progeny seed, or a plant grown therefrom, to produce modified progeny seed; or b) genome editing the plant or plant part of claim 1 to produce modified seed and crossing or selfing the modified seed to produce modified progeny seed.
  10. 10 . A method for producing nucleic acids, the method comprising isolating nucleic acids from the seed, plant, plant part, or plant cell of claim 1 .
  11. 11 . A converted seed, plant, plant part or plant cell of canola line 4PWGT51R, representative seed of the canola line 4PWGT51R having been deposited under NCMA accession number 202309004, wherein the converted seed, plant, plant part or plant cell comprises fewer than six locus conversions, and wherein the plant or a plant grown from the converted seed, plant part or plant cell comprises the fewer than six locus conversions and otherwise comprises all of the physiological and morphological characteristics of canola line 4PWGT51R when grown under the same environmental conditions.
  12. 12 . The converted seed, plant, plant part or plant cell of claim 11 , wherein the fewer than six locus conversions confers a property selected from the group consisting of male sterility, a site for site-specific recombination, abiotic stress tolerance, altered phosphate, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide tolerance, insect resistance and disease resistance.
  13. 13 . A Brassica seed produced by crossing the plant or plant part of claim 11 with a different Brassica plant.
  14. 14 . A hybrid Brassica plant or plant part produced by growing the seed of claim 13 , wherein the plant part comprises at least one cell of the hybrid Brassica plant.
  15. 15 . A method for producing a second Brassica plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 14 to produce the second Brassica plant.
  16. 16 . A method of making a commodity plant product comprising carbohydrate, silage, oil or protein, the method comprising producing the commodity plant product from the Brassica plant or plant part of claim 14 .
  17. 17 . A method for producing nucleic acids, the method comprising isolating nucleic acids from the converted seed, plant, plant part, or plant cell of claim 11 .
  18. 18 . A seed of canola line 4PWGT51R, further comprising a transgene, wherein the transgene was introduced into canola line 4PWGT51R by backcrossing or genetic transformation, wherein representative seed of the canola line 4PWGT51R have been deposited under NCMA accession number 202309004, and wherein a plant grown from the seed comprises a trait conferred by the transgene and otherwise all of the physiological and morphological characteristics of canola line 4PWGT51R when grown under the same environmental conditions.
  19. 19 . A method of producing progeny seed, the method comprising crossing a plant grown from the seed of claim 18 with itself or a second plant to produce progeny seed.
  20. 20 . A method of making a commodity plant product comprising silage, carbohydrate, oil or protein, the method comprising producing the commodity plant product from an F1 plant or grain produced thereon grown from the seed of claim 18 .

Description

BACKGROUND A novel rapeseed line designated 4PWGT51R is the result of years of careful breeding and selection. Since such variety is of high quality and possesses a relatively low level of erucic acid in the vegetable oil component and a relatively low level of glucosinolate content in the meal component, it can be termed “canola” in accordance with the terminology commonly used by plant scientists. SUMMARY A novel Brassica napus line designated 4PWGT51R is provided. Seed of canola line 4PWGT51R, plants of canola line 4PWGT51R, plant parts of canola line 4PWGT51R, and processes for making a canola plant that comprise crossing canola line 4PWGT51R with another Brassica plant are provided. Also provided is 4PWGT51R with cytoplasm comprising a gene or genes that cause male sterility. Processes for making a plant containing in its genetic material one or more traits introgressed into 4PWGT51R through backcross conversion and/or transformation, and to the seed, plant and plant parts produced thereby are provided. A hybrid canola seed, plant or plant part can be produced by crossing the line 4PWGT51R or a locus conversion of 4PWGT51R with another Brassica plant. Definitions In the description and examples which follow, a number of terms are used. The following definitions and evaluation criteria are provided. Anther Arrangement. The general disposition of the anthers in typical fully opened flowers is observed. Anther Fertility. The ability of a plant to produce pollen; measured by pollen production. 1=sterile, 9=all anthers shedding pollen (vs. Pollen Formation which is amount of pollen produced). Chlorophyll Content. The typical chlorophyll content of the mature seeds is determined by using methods recommended by the Western Canada Canola/Rapeseed Recommending Committee (WCC/RRC). 1=low (less than 8 ppm), 2=medium (8 to 15 ppm), 3=high (greater than 15 ppm). Also, chlorophyll could be analyzed using NIR (Near Infrared) spectroscopy as long as the instrument is calibrated according to the manufacturer's specifications. CMS. Abbreviation for cytoplasmic male sterility. Cotyledon. A cotyledon is a part of the embryo within the seed of a plant; it is also referred to as a seed leaf. Upon germination, the cotyledon may become the embryonic first leaf of a seedling. Cotyledon Length. The distance between the indentation at the top of the cotyledon and the point where the width of the petiole is approximately 4 mm. Cotyledon Width. The width at the widest point of the cotyledon when the plant is at the two to three-leaf stage of development. 3=narrow, 5=medium, 7=wide. If scores are averaged, a value with decimal places may be provided. CV %: Abbreviation for coefficient of variation. Cytoplasmic Conversion. A plant that has been developed by transferring the cytoplasm of a plant to a variety of interest. This can be done through crossing the variety of interest to a plant that has the desired cytoplasm and backcrossing to the variety of interest. The cytoplasm will be transferred through the female parent. The result would be the genome of the variety of interest with the cytoplasm of another plant, generally the cytoplasm from the other plant will confer male sterility. Disease Resistance: Resistance to various diseases is evaluated and is expressed on a scale of 0=not tested, 1=resistant, 3=moderately resistant, 5=moderately susceptible, 7=susceptible, and 9=highly susceptible. Erucic Acid Content: The percentage of the fatty acids in the form of C22:1.as determined by one of the methods recommended by the WCC/RRC, being AOCS Official Method Ce 2-66 Preparation of Methyl esters of Long-Chain Fatty Acids or AOCS Official Method Ce 1-66 Fatty Acid Composition by Gas Chromatography. F1 Progeny. A first-generation progeny plant produced by crossing a plant of canola variety 4PWGT51R with a plant of another canola plant. Fatty Acid Content: The typical percentages by weight of fatty acids present in the endogenously formed oil of the mature whole dried seeds are determined. During such determination the seeds are crushed and are extracted as fatty acid methyl esters following reaction with methanol and sodium methoxide. Next the resulting ester is analyzed for fatty acid content by gas liquid chromatography using a capillary column which allows separation on the basis of the degree of unsaturation and fatty acid chain length. Flower Bud Location. A determination is made whether typical buds are disposed above or below the most recently opened flowers. 1=buds above most recently opened flowers, 9=buds below most recently opened flowers. Flower Date 50%. (Same as Time to Flowering) The number of days from planting until 50% of the plants in a planted area have at least one open flower. Flower Petal Coloration. The coloration of open exposed petals on the first day of flowering is observed. 1=white, 2=light yellow, 3=medium yellow, 4=dark yellow, 5=orange, 6=other. Frost Tolerance (Spring Type Only). The ability of young p