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US-12616135-B2 - Variety corn line TPFX7814

US12616135B2US 12616135 B2US12616135 B2US 12616135B2US-12616135-B2

Abstract

The present invention provides an inbred corn line designated TPFX7814, methods for producing a corn plant by crossing plants of the inbred line TPFX7814 with plants of another corn plant. The invention further encompasses all parts of inbred corn line TPFX7814, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line TPFX7814, and plants produced according to these methods.

Inventors

  • Thomas Joseph Prest

Assignees

  • SYNGENTA CROP PROTECTION AG

Dates

Publication Date
20260505
Application Date
20231030

Claims (20)

  1. 1 . A seed of maize variety TPFX7814, wherein representative seed of said maize variety TPFX7814 has been deposited under ATCC Accession Number PTA-127669.
  2. 2 . A plant of maize variety TPFX7814, wherein representative seed of said maize variety TPFX7814 has been deposited under ATCC Accession Number PTA-127669.
  3. 3 . A plant part of the plant of claim 2 .
  4. 4 . The plant part of claim 3 , wherein said part is a pollen grain, a silk, a protoplast, a cell, a tassel, an anther or an ovule.
  5. 5 . A maize seed produced on the plant of claim 2 .
  6. 6 . A maize plant having of the physiological and morphological characteristics of the plant according to claim 2 and further comprising an additional trait, wherein the additional trait is selected from the group consisting of water stress resistance, waxy starch, male sterility or restoration of male fertility, modified carbohydrate metabolism, modified protein metabolism, modified fatty acid metabolism, altered starch, thermotolerant amylase, herbicide resistance, insect resistance, nematode resistance, bacterial disease resistance, fungal disease resistance, and viral disease resistance.
  7. 7 . The plant of claim 6 wherein the additional trait is conferred by introducing a transgene.
  8. 8 . A converted seed, plant, plant part or plant cell of maize variety TPFX7814, representative seed of the maize variety TPFX7814 having been deposited under ATCC accession number PTA-127669, wherein the converted seed, plant, plant part or plant cell comprises a transgene locus conversion, and wherein the plant or a plant grown from the converted seed, plant part or plant cell comprises the transgene locus conversion and otherwise comprises the phenotypic characteristics of maize variety TPFX7814 listed in Table 1 when grown under the same environmental conditions.
  9. 9 . A process for producing maize seed, said process comprising crossing the maize plant of claim 2 with a different maize plant, and harvesting the seed.
  10. 10 . An F1 maize seed produced by the process of claim 9 .
  11. 11 . An F1 maize plant produced by germinating the seed of claim 10 .
  12. 12 . A method of producing a genetic marker profile comprising extracting nucleic acids from the seed of claim 10 or the plant germinated from said seed and genotyping said nucleic acids at one or more genetic loci, thereby producing a genetic marker profile.
  13. 13 . A method of plant breeding comprising a) isolating nucleic acids from the seed of claim 10 , b) identifying one or more polymorphisms from the isolated nucleic acids, and c) selecting a plant obtained from said seed having said one or more polymorphisms, wherein the plant is used in a plant breeding method.
  14. 14 . A method of plant breeding comprising a) isolating nucleic acids from the plant of claim 11 , b) identifying one or more polymorphisms from the isolated nucleic acids, and c) selecting a plant having said one or more polymorphisms, wherein the plant is used in a plant breeding method.
  15. 15 . A process of introducing an additional trait into maize plant TPFX7814 comprising: (a) crossing TPFX7814 plants grown from TPFX7814 seeds, representative seeds deposited under ATCC Accession Number PTA-127669, with plants of another maize variety that comprise an additional trait to produce hybrid progeny plants, (b) selecting hybrid progeny plants that have the additional trait to produce selected hybrid progeny plants; (c) crossing the selected progeny plants with the TPFX7814 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the additional trait to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) at least three or more times to produce backcross progeny plants that comprise the additional trait and all of the physiological and morphological characteristics of maize inbred plant TPFX7814 when grown in the same environmental conditions.
  16. 16 . A plant produced by the process of claim 15 .
  17. 17 . A method of producing a maize plant derived from the inbred plant TPFX7814, the method comprising the steps of (a) growing the plant of claim 11 ; (b) crossing said plant with itself or a different plant to produce a seed of a progeny plant; (c) repeating step (b) at least one or more times; and (d) growing said progeny plant from said seed and crossing the progeny plant with itself or a different plant to produce a maize plant derived from the inbred plant TPFX7814.
  18. 18 . A method for developing a second maize variety in a maize plant breeding program, comprising applying plant breeding techniques wherein said techniques comprise recurrent selection, backcrossing, pedigree breeding, marker enhanced selection, haploid/double haploid production, or transformation to the maize plant of claim 11 , wherein application of said techniques results in development of a second maize variety.
  19. 19 . A method of producing a commodity plant product comprising growing the plant from the seed of claim 10 , and producing said commodity plant product comprising protein concentrate, protein isolate, starch, meal, flour or oil therefrom.
  20. 20 . A method of producing a maize plant with doubled haploid chromosomes from the maize variety TPFX7814 the method comprising: (a) crossing the plant of claim 11 with an inducer maize plant to produce a progeny with haploid chromosomes; and (b) doubling the haploid chromosomes in the progeny to produce a maize plant with doubled haploid chromosomes.

Description

FIELD OF THE INVENTION This invention is in the field of corn breeding. Specifically, the present invention provides a maize plant and its seed designated TPFX7814, as well as derivatives and hybrids thereof. BACKGROUND OF THE INVENTION Maize (or corn; Zea mays L.) plant breeding is a process to develop improved maize germplasm in an inbred or hybrid plant. Maize plants can be self-pollinating or cross pollinating. Self-pollination for several generations produces homozygosity at almost all gene loci, forming a uniform population of true breeding progeny, known as inbreds. Hybrids are developed by crossing two homozygous inbreds to produce heterozygous gene loci in hybrid plants and seeds. In this process, the inbred is emasculated and the pollen from the other inbred pollinates the emasculated inbred. Emasculation of the inbred can be done by chemical treatment of the plant, detasseling the seed parent, or the parent inbred can comprise a male sterility trait or transgene imparting sterility, eliminating the need for detasseling. This emasculated inbred, often referred to as the female, produces the hybrid seed, F1. The hybrid seed that is produced is heterozygous. However, the grain produced by a plant grown from F1 hybrid seed is referred to as F2 grain. F2 grain which is a plant part produced on the F1 plant will comprise segregating maize germplasm, even though the hybrid plant is heterozygous. Such heterozygosity in hybrids results in robust and vigorous plants. Inbred plants on the other hand are mostly homozygous, rendering them less vigorous. Inbred seed can be difficult to produce due to such decreased vigor. However, when two inbred lines are crossed, the resulting hybrid plant shows greatly increased vigor and seed yield compared to open pollinated, segregating maize plants. An important consequence of the homozygosity and homogeneity of inbred maize lines is that all hybrid seed and plants produced from any cross of two such lines will be the same. Thus the use of inbreds allows for the production of hybrid seed that can be readily reproduced. There are numerous stages in the development of any novel, desirable plant germplasm. Plant breeding begins with the analysis and definition of problems and weaknesses of the current germplasm, the establishment of program goals, and the definition of specific breeding objectives. The next step is selection of germplasm that possess the traits to meet the program goals. The aim is to combine in a single variety an improved combination of desirable traits from the parental germplasm. These important traits may include, for example, higher yield, resistance to diseases, fungus, bacteria and insects, better stems and roots, tolerance to drought and heat, improved nutritional quality, and better agronomic characteristics. Choice of breeding methods depends on the mode of plant reproduction, the heritability of the trait(s) being improved, and the type of cultivar used commercially (e.g., F1 hybrid cultivar, pure line cultivar, etc.). For highly heritable traits, a choice of superior individual plants evaluated at a single location may be effective, whereas for traits with low heritability, selection can be based on mean values obtained from replicated evaluations of families of related plants. Popular selection methods commonly include pedigree selection, modified pedigree selection, mass selection, and recurrent selection. The complexity of inheritance influences the choice of breeding method. Backcross breeding is used to transfer one or a few favorable genes for a highly heritable trait into a desirable cultivar. This approach has been used extensively for breeding disease-resistant cultivars and introducing transgenic events into maize germplasm. Thus, backcross breeding is useful for transferring genes for a simply inherited, highly heritable trait into a desirable homozygous cultivar or inbred line which is the recurrent parent. The source of the trait to be transferred is called the donor parent. After the initial cross, individuals possessing the phenotype of the donor parent are selected and repeatedly crossed (backcrossed) to the recurrent parent. The resulting plant is expected to have the attributes of the recurrent parent (e.g., cultivar) and the desirable trait transferred from the donor parent. Each breeding program generally includes a periodic, objective evaluation of the efficiency of the breeding procedure. Evaluation criteria vary depending on the goals and objectives, but should include gain from selection per year based on comparisons to an appropriate standard, overall value of the advanced breeding lines, and number of successful cultivars produced per unit of input (e.g., per year, per dollar expended, etc.). The ultimate objective of commercial corn breeding programs is to produce high yield, agronomically sound plants that perform well in particular regions of the U.S. Corn Belt, such as a plant of this invention. SUMMARY OF THE INV