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US-12616643-B2 - Method of preparing new biomaterials for dental pulp regeneration and a composition of new biomaterials prepared accordingly

US12616643B2US 12616643 B2US12616643 B2US 12616643B2US-12616643-B2

Abstract

Proposed are a method of preparing new biomaterials for dental pulp regeneration and a composition of new biomaterials prepared accordingly. The composition of new biomaterials prepared according to the method of preparing new biomaterials for dental pulp regeneration by mixing a first precursor containing calcium silicate-based cement (CSC) at an appropriate concentration and a second precursor containing a secretome of dental pulp-derived mesenchymal stem cells in an appropriate ratio and then drying the resulting mixture under appropriate drying conditions is characterized by further improving the osteogenic differentiation ability of dental pulp stem cells compared to existing materials while maintaining the microhardness of the composition to an appropriate standard. In addition, the composition is advantageous in that an aesthetic effect is excellent by not causing tooth discoloration.

Inventors

  • Sin Young Kim

Assignees

  • THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Dates

Publication Date
20260505
Application Date
20231026
Priority Date
20221103

Claims (12)

  1. 1 . A method of preparing new biomaterials for dental pulp regeneration, the method comprising: preparing a first precursor comprising a calcium silicate-based cement (CSC); preparing a second precursor comprising a secretome of a pulp-derived mesenchymal stem cell; preparing a secretome-mixed CSC preliminary composition by mixing the first and second precursors; and drying the preliminary composition.
  2. 2 . The method of claim 1 , wherein the CSC comprises one or more types selected from the group consisting of dentin restorative material, hydraulic calcium zirconium complex, and premixed mineral trioxide aggregate.
  3. 3 . The method of claim 1 , wherein the CSC of the first precursor has a concentration of 3 to 7 mg/mL.
  4. 4 . The method of claim 1 , wherein the secretome has a concentration of 3 wt % to 7 wt % with respect to 100 wt % of the total resulting mixture of the first and second precursors.
  5. 5 . The method of claim 1 , wherein the drying of the preliminary composition is performed for 7 to 9 days.
  6. 6 . The method of claim 5 , wherein the drying of the preliminary composition comprises: performing primary drying on the preliminary composition at room temperature for 2 to 3 days; and performing secondary drying on the resulting product obtained through the primary drying, at a temperature of 36° C. to 38° C. for 5 to 6 days.
  7. 7 . A composition of new biomaterials for dental pulp regeneration, the composition prepared by the method of claim 1 .
  8. 8 . The composition of claim 7 , wherein the CSC comprises one or more types selected from the group consisting of dentin restorative material, hydraulic calcium zirconium complex, and premixed mineral trioxide aggregate.
  9. 9 . The composition of claim 7 , wherein the CSC of the first precursor has a concentration of 3 to 7 mg/mL.
  10. 10 . The composition of claim 7 , wherein the secretome has a concentration of 3 wt % to 7 wt % with respect to 100 wt % of the total resulting mixture of the first and second precursors.
  11. 11 . The composition of claim 7 , wherein the drying of the preliminary composition is performed for 7 to 9 days.
  12. 12 . The composition of claim 11 , wherein the drying of the preliminary composition comprises: performing primary drying on the preliminary composition at room temperature for 2 to 3 days; and performing secondary drying on the resulting product obtained through the primary drying, at a temperature of 36° C. to 38° C. for 5 to 6 days.

Description

CROSS REFERENCE TO RELATED APPLICATION The present application claims priority to Korean Patent Applications 10-2022-0145171, filed Nov. 3, 2022 and No. 10-2023-0121994, filed Sep. 13, 2023, the entire contents of which is incorporated herein for all purposes by this reference. FIELD Proposed are a method of preparing new biomaterials for dental pulp regeneration and a composition of new biomaterials prepared accordingly. BACKGROUND Dental caries was the second most common disease among teenagers in Korea in 2019, showing a rapidly growing trend with an average annual increase rate of 7.4%. Root canal treatment is often required for advanced dental caries. However, compared to the traditional root canal treatment for permanent teeth that is performed all the way to the root apex, the concept of root canal treatment for immature permanent teeth in adolescence is not well-established. Hence, in the case of immature permanent teeth with an open root apex, research on regenerative root canal treatment is in progress by focusing on the regeneration of the dental pulp and periapical root tissue by performing root canal treatment only on the upper side, allowing stem cells from the apical papilla (SCAP) to be left at the root end. However, according to recent research on regenerative root canal treatment, in the case of existing materials used in such treatment, complete setting takes a long time. In addition, with longer setting time, the existing materials come into contact with blood or tissue fluids, causing discoloration and thus deteriorating the aesthetic effect. Furthermore, there is a disadvantage leading to a decrease in compressive strength. DOCUMENT OF RELATED ART Patent Document (Patent Document 1) Korean Patent Application Publication No. 10-2010-0103821 SUMMARY Proposed are a method of preparing a composition for dental pulp regeneration by mixing a first precursor containing calcium silicate-based cement (CSC) at an appropriate concentration and a second precursor containing a secretome of dental pulp-derived mesenchymal stem cells and then drying the resulting mixture under predetermined conditions, and a composition for dental pulp regeneration. A method of preparing new biomaterials for dental pulp regeneration, according to one aspect, includes: preparing a first precursor containing a calcium silicate-based cement (CSC); preparing a second precursor containing a secretome of dental pulp-derived mesenchymal stem cells; preparing a secretome-mixed CSC preliminary composition by mixing the first and second precursors; and drying the preliminary composition. The CSC may include one or more types selected from the group consisting of Biodentine, RetroMTA, and Endocem MTA Premixed. The CSC of the first precursor may have a concentration of 3 to 7 mg/mL. The secretome may have a concentration of 3 to 7 wt % with respect to 100 wt % of the total resulting mixture of the first and second precursors. The drying of the preliminary composition may be performed for 7 to 9 days. The drying of the preliminary composition may include: performing primary drying on the preliminary composition at room temperature for 2 to 3 days; and performing secondary drying on the resulting product obtained through the primary drying, at a temperature of 36° C. to 38° C. for 5 to 6 days. A composition of new biomaterials for dental pulp regeneration, according to another aspect, contains a CSC and a secretome of a dental pulp-derived mesenchymal stem cell, prepared by the method described above. A composition of new biomaterials prepared according to a method of preparing new biomaterials for dental pulp regeneration from first and second precursors contained in appropriate concentrations by being subjected to appropriate drying conditions is characterized by further improving osteogenic differentiation ability and viability of dental pulp stem cells compared to existing materials while maintaining the hardness of the composition to an appropriate standard. In addition, the composition is advantageous in that an aesthetic effect is excellent without causing tooth discoloration. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing results of Vickers hardness values of a composition for dental pulp regeneration with varying secretome concentrations of pulp-derived mesenchymal stem cells; FIG. 2 is a graph showing results of Vickers hardness values of a composition for dental pulp regeneration with varying drying conditions; FIG. 3 is a graph showing measurement results of the total amount of alkaline phosphatase (Days 3 and 6) in dental pulp stem cells when adding a composition for dental pulp regeneration; FIG. 4 is a graph showing measurement results of the amount of alkaline phosphatase on Day 3 in dental pulp stem cells when adding a composition for dental pulp regeneration; FIG. 5 is a graph showing measurement results of the amount of alkaline phosphatase on Day 6 in dental pulp stem cells when adding a compositio