US-12616717-B2 - Regenerative CO2 treatment apparatus and method

US12616717B2US 12616717 B2US12616717 B2US 12616717B2US-12616717-B2

Abstract

The present technology provides regenerative treatment methods and apparatus to promote regeneration of target biological tissue. Regenerative treatments include the subcutaneous and cutaneous application of carbon dioxide. Regenerative treatment methods include an initial treatment phase and a maintenance treatment phase. Regenerative treatment apparatus include effervescent mineral compositions for cutaneous application of carbon dioxide.

Inventors

Renee Tornatore

Assignees

Renee Tornatore

Dates

Publication Date: 20260505
Application Date: 20200709

Claims (19)

1 . A regenerative treatment method for treating target biological tissue within a target area of a patient, the method comprising: administering an initial treatment phase comprising administering at least one initial phase therapy session and administering at least one initial phase personal administration, wherein administering each initial phase therapy session includes at least: subcutaneously applying carbon dioxide in the target area; and simultaneously administering electrical stimulation to at least the target area and carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid, by: providing a first water bath and a second water bath, each water bath containing a volume of water and the effervescent mineral composition; and providing a current generator having a positive lead connected to the current generator and a negative lead connected to the current generator, the positive lead being further connected to the first water bath, and the negative lead being further connected to the second water bath; and generating a biphasic current using the current generator; and wherein administering each initial phase personal administration consists of applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid; and administering a maintenance treatment phase after the initial treatment phase, the maintenance phase comprising administering at least one maintenance phase therapy session and administering at least one maintenance phase personal administration, wherein administering each maintenance phase therapy session includes at least subcutaneously applying carbon dioxide in the target area and administering each maintenance phase personal administration consists of applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid.
2 . The regenerative treatment method of claim 1 , wherein administering each initial phase therapy session further comprises, subsequent to the subcutaneously applying carbon dioxide in the target area, applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid.
3 . The regenerative treatment method of claim 1 , wherein administering each therapy session further comprises physically manipulating the target area subsequent to subcutaneously applying carbon dioxide in the target area.
4 . The regenerative treatment method of claim 1 , wherein administering each therapy session further includes administering a secondary treatment to the target area.
5 . The regenerative treatment method of claim 4 , wherein administering the secondary treatment comprises administering electrical stimulation to at least the target area.
6 . The regenerative treatment method of claim 4 , wherein administering the secondary treatment comprises administering extracorporeal shock wave therapy to at least the target area.
7 . The regenerative treatment method of claim 1 , wherein administering the at least one maintenance phase therapy session further includes simultaneously administering carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid, and electrical stimulation to at least the target area.
8 . The regenerative treatment method of claim 7 , wherein the simultaneously administering carbon dioxide to the outer surface of the skin in the target area and electrical stimulation to at least the target area includes: providing a first water bath and a second water bath, each water bath containing a volume of water and the effervescent mineral composition; and providing a current generator having a positive lead connected to the current generator and a negative lead connected to the current generator, the positive lead being further connected to the first water bath, and the negative lead being further connected to the second water bath; and generating a biphasic current using the current generator.
9 . The regenerative treatment method of claim 1 , wherein the effervescent mineral composition further comprises: at least one fat facilitator.
10 . The regenerative treatment method of claim 1 , wherein the bicarbonate and the acid are present in a ratio of 3:2.
11 . The regenerative treatment method of claim 9 , wherein the bicarbonate comprises at least one of sodium hydrogen carbonate and sodium carbonate.
12 . The regenerative treatment method of claim 9 , wherein the acid comprises at least one of citric acid, lactic acid, and vinegar.
13 . The regenerative treatment method of claim 9 , wherein the at least one fat facilitator comprises at least one of a moisturizer, lactose, and calcium silicate.
14 . The regenerative treatment method of claim 9 , wherein the effervescent mineral composition further comprises a vasodilator.
15 . The regenerative treatment method of claim 14 , wherein the vasodilator comprises nitric oxide.
16 . The regenerative treatment method of claim 9 , wherein the effervescent mineral composition is provided in the form of a compressed solid or a powder.
17 . The regenerative treatment method of claim 9 , wherein the effervescent mineral composition is provided within an enclosed pack having a water permeable layer.
18 . The regenerative treatment method of claim 1 , wherein generating a biphasic current using the current generator comprises generating a biphasic current with a frequency of 8 Hertz and a voltage of +0 to 36V, and −0 to 36V.
19 . A regenerative treatment method for treating target biological tissue within a target area of a patient, the method comprising: administering an initial treatment phase comprising administering at least one initial phase therapy session and administering at least one initial phase personal administration; wherein administering each initial phase therapy session includes subcutaneously applying carbon dioxide in the target area, and subsequently administering electrical stimulation to at least the target area and simultaneously applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid by: providing a first water bath and a second water bath, each water bath containing a volume of water and the effervescent mineral composition; and providing a current generator having a positive lead connected to the current generator and a negative lead connected to the current generator, the positive lead being further connected to the first water bath, and the negative lead being further connected to the second water bath; and generating a biphasic current using the current generator; and wherein administering each initial phase personal administration consists of applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the outer surface of the skin in the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid; and administering a maintenance treatment phase after the initial treatment phase, the maintenance phase comprising administering at least one maintenance phase therapy session and administering at least one maintenance phase personal administration; wherein administering each maintenance phase therapy session includes subcutaneously applying carbon dioxide in the target area, and subsequently applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the outer surface of the skin in the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid; and wherein administering each maintenance phase personal administration consists of applying carbon dioxide to the outer surface of the skin in the target area by applying activated effervescent mineral composition to the target area, wherein the effervescent mineral composition comprises bicarbonate and an acid.

Description

RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 62/872,749, entitled “CO2 Treatment Apparatus and Method,” filed Jul. 11, 2019, and the disclosure thereof is hereby incorporated by reference in its entirety. FIELD OF THE INVENTION The present technology relates to the use of carbon dioxide (CO2) for regenerative therapeutic purposes, and provides certain methods of regeneration treatment, as well as devices for implementing treatment and for the delivery of carbon dioxide to targeted body tissues. BACKGROUND The regeneration of damaged biological tissues is of significant interest. Regeneration is distinguishable from processes of scarring and healing, and regenerative medicine focuses on ways to optimize tissue regeneration by therapeutically manipulating the body's natural reaction of fibroblast deposition and scar formation in response to injury or disease. Various strategies have been developed for regenerative treatments to promote tissue regeneration, including for example, the use of biomaterials as scaffolds, cells, and combinations of biomaterials and cells. Many studies have been conducted in both plants and animals to try to understand how regenerative processes work. For example, as described in “Nature's Electric Potential: A systematic Review of the Role of Bioelectricity in Wound Healing and Regenerative Process in Animals, Humans, and Plants, from the Frontiers in Physiology,” Shena E. B. Tyler (Frontiers in Physiology, Sep. 4, 2017), regenerative processes can be broken down into five phases. In the first phase, there is an injury (wound), or disruption to the tissue cells, such as by 1) spatial variations in ion channels or pumps, or 2) disruption in gap junctions. This creates extracellular ionic current flow, and established voltage gradients. This essentially creates a bioelectric voltage gradient. Bioelectricity is the flow of current carried by mobile charged ions, across cell membrane and along exterior and interior ionic environments of cells. The body then responds by initiating processes to either regenerate or scar and repair. In the second phase, changes in polarity are communicated. The signals may be carried via: Electrical Fields—presence of dipole with no immediate barrier; Membrane potential—presence of dipole across barrier with selective permeability created by ion gradients across a membrane via action of ion channels and pumps; Flux—flow of ions through channels or pumps per unit of time; PH gradients—by proton pumps to modify H+ gradients. The article “Bioelectric Signaling in Regeneration: Mechanisms of Ionic Controls of Growth and Form,” McLaughlin et al., (Developmental Biology 433 (2018)), explains that transmembrane potentials, fluxes of individual ions, and iso-electric cell compartments that are established by gap junctions, convey information to at least target cells. Further, as described in the article “Molecular Bioelectricity: How Endogenous Voltage Potentials Control Cell Behavior And Instruct Pattern Regulation in Vivo,” Michael Levin (Molecular Biology of the Cell, Dec. 1, 2014), it is believed that this signaling modality is used to process and transmit information about regenerative parameters such as cell type, tissue size, positional information, axial polarity, and organ identity. The third phase involves mechanisms acting as receptors for the signals, such as proton pumps and other cells. Biophysical transduction mechanisms may include: 1) Voltage sensing domain, 2) loss of intracellular K+, Electroosmosis 4) Voltage gating of signaling molecular transport, 5) Ca++ influx, 6). Secondary response, amplification, transcriptional effectors may include: 1) integrin, 2) Slug/Sox10, 3) Notch, 4) NF-kB, 5) PTEN. The fourth phase includes ionic flux, which involves the downstream activation of a number of gene responses, which evoke transcriptional cascades involved in the control of morphologies and regeneration. The fifth phase involves initiating a cascade of events through cellular death and proliferation, and differentiation of stem cells to regenerate parts, pieces, or entire modules. It is believed that when bioelectric patterns are specifically disrupted, predictable and coherent changes in morphogenesis occur. It is also believed that the necessary parameter is voltage potential, without regard necessarily to any one channel gene (which could have had scaffold or binding roles) or even any one ion type (which could have had chemical, not electrical, roles). Accordingly, achieving the correct voltage in the target biological tissue may trigger the regenerative cascade to regenerate parts, pieces, or entire modules. One example of a treatment that has been developed for use regenerative therapies in humans is carboxytherapy. Typically, carboxytherapy uses a series of small injections with a thin needle attached to a tube which delivers tiny quantities of medical grade carbon dioxide below the skin's surfa