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US-12617014-B2 - AU, AG and rich phytochemical payload nanomaterials, antiviral/antibacterial products and synthesis methods

US12617014B2US 12617014 B2US12617014 B2US 12617014B2US-12617014-B2

Abstract

A nanomaterial is gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin, Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral/antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral/antibacterial exceeding a 3 log 10 reduction in viral titer.

Inventors

  • Kattesh V. Katti
  • Kavita K. Katti

Assignees

  • THE CURATORS OF THE UNIVERSITY OF MISSOURI

Dates

Publication Date
20260505
Application Date
20220510

Claims (11)

  1. 1 . A nanomaterial comprising a gold nanoparticle or silver nanoparticle having a nanoparticle surface that consists of shikimic acid-3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid having the structure of: wherein the particle has a core size of 30-40 nm.
  2. 2 . The nanomaterial of claim 1 , wherein the particle with the surface has a size of 89±50 nm.
  3. 3 . The nanomaterial of claim 1 wherein the particle is a gold nanoparticle and has a hydrodynamic size of 122.5±5 nm.
  4. 4 . The nanomaterial of claim 1 , wherein the particle is a silver nanoparticle and has a hydrodynamic size of 185-205 nm.
  5. 5 . A nanomaterial comprising a silver nanoparticle having a nanoparticle surface that consists of shikimic acid-3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid having the structure of: wherein the particle has a hydrodynamic size of 185-205 nm.
  6. 6 . The nanomaterial of claim 1 , wherein the particle is a silver nanoparticle and has a hydrodynamic size of 200-220 nm.
  7. 7 . A method for synthesizing a nanomaterial, the method comprising obtaining a phytoextract solution from sweet gum plant leaves, sweet gum stems or powder of a sweet gum plant, filtering the solution and reacting the solution with gold or silver salts to produce the nanomaterial, wherein the phytoextract comprises isolated Shikimic Acid, and the method comprises a preliminary step of extracting Shikimic Acid by boiling one of stems of a sweet gum plant, leaf powder of a sweet gum plant, and the leaves of a sweet gum plant in DI water for a predetermined period and filtering the solution after boiling to obtain Shikimic Acid extract.
  8. 8 . The method of claim 7 , wherein the obtaining comprises washing the sweet gum plant leaves to remove dust particles, cutting the sweet gum plant leaves into pieces; adding gum Arabic to distilled water; and adding the pieces to the distilled water; and the reacting comprises heating and stirring the distilled water with the pieces to a temperature of at least 100° C., then adding the gold or silver salts and stirring for a period of time until nanoparticle formation occurs.
  9. 9 . The method of claim 7 , wherein the reacting is conducted in a temperature range of 60° C.-100° C.
  10. 10 . The method of claim 9 , wherein the reacting is conducted with Gum Arabic.
  11. 11 . An antiviral/antibacterial agent comprising sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral/antibacterial exceeding a 3 log 10 reduction in viral titer, in an oral consumption carrier for use as a pharmaceutical to treat viruses.

Description

PRIORITY CLAIM AND REFERENCE TO RELATED APPLICATION The application claims priority under 35 U.S.C. § 119 and all applicable statutes and treaties from prior U.S. provisional application Ser. No. 63/297,937, which was filed Jan. 10, 2022, and from U.S. provisional application Ser. No. 63/189,420, which was filed May 17, 2021. TECHNICAL FIELD A field of the invention is composite nanomaterials. Example applications of the invention include biomedical applications such as disease treatment and disease detection. Another field of the invention is antiviral/antibacterial products and treatments. Another field of the invention is nanotechnology BACKGROUND Antiviral agents are important to protect human civilization from pandemics. Effective antivirals can provide an important defense to the spread of viral based pandemics, including the family of SARS-COV-2 (which causes COVID-19) and influenza viruses (which causes the seasonal flu) viruses. Bulk silver itself has been recognized and used over time in antifungal, antibacterial and anti-viral applications. Bulk silver itself is not suitable as an antiviral/antibacterial agent. Chitosan-stabilized gold nanoparticles have been investigated for their antifungal properties against the pathogen Fusarium oxysporum. Lipsa et al., valuation of the Antifungal Activity of Gold-Chitosan and Carbon Nanoparticles on Fusarium oxysporum,” Agronomy 2020, 10, 1143. Antifungal properties were attributed to the efficacy of the chitosan and how it was enhanced via the combination of gold-chitosan nanoparticles. The targeted application is to control fungus in agricultural fields. As noted, the properties of the particles are particular to the coating and reduction agent. Gold and silver nanoparticles were fabricated via a P. rhodozyma cell-free extract. Rónavári et al, “Biosynthesized silver and gold nanoparticles are potent antimycotics against opportunistic pathogenic yeasts and dermatophytes,” International Journal of Nanomedicine 2018:13, 695-703. The described testing reported that the particles were effective against specific mycois-causing fungal species, except for C. tropicalis. AgNPs obtained by chemical reduction using coffee and green tea extracts were reported to have antimicrobial efficiency. Rónavári A, Kovacs D, Igaz N, et al. Biological activity of green-synthesized silver nanoparticles depends on the applied natural extracts: a comprehensive study. Int J Nanomedicine. 2017; 12:871-883. As indicated, specific synthesis routes provide unpredictable biological activities. SUMMARY OF THE INVENTION A preferred embodiment provides a nanomaterial of gold or silver nanoparticle having a nanoparticle surface that consists of one or more of the following extracts from sweet gum leaves: shikimic acid, sweet gum leaf family of Catechins, sweet gum leaf family of Quercetin Sweroside, Afzelechin, p-Coumaric Acid, Epiafzelechin Trimethyl Ether, Myricetin, Naringenin, Phloretin, Procyanidin B1 and related polyphenols, flavonoids and alkaloids. An antiviral/antibacterial agent includes sweet gum mediated silver nanoparticles having a core size and in a concentration having efficacy as an antiviral exceeding a 3 log 10 reduction in viral titer. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments provide silver nanoparticle antiviral/antibacterial products and pharmaceuticals. Products include antiviral/antibacterial sprays, antiviral/antibacterial wipes, and antiviral/antibacterial cotton textiles. A pharmaceutical includes sweet gum plant phytochemical-mediated silver nanoparticles in an oral consumption carrier for use as a pharmaceutical to treat viruses, including the family of SARS-COV-2 (which causes COVID-19) and influenza viruses (which causes the seasonal flu). Preferred silver nanoparticle antiviral/antibacterial agents of the invention demonstrated a 3.50 Log 10 reduction in viral titer (99.97%) at a contact time of 1 minute and a corresponding 3.75 Log 10 reduction in viral titer (99.98%) at a contact time of 5 minutes, as compared to the titer of the corresponding Plate Recovery Control. Preferred embodiments also include green and zero carbon fabrication methods of silver nanoparticle antiviral/antibacterial agents using sweet gum plant mediated formation. Preferred silver nanoparticles are encapsulated with antiviral/antibacterial phytochemicals. The construct provides the for neutralizing deadly COVID-19 and related viruses by penetrating the viral envelope composed of a lipid bilayer membrane, in COVID-19, in which the glycoprotein spikes are anchored encloses the nucleocapsids. The neutralization of the virus can occur via dual actions of the antiviral/antibacterial silver nanoparticles as well as the cargo of antiviral/antibacterial phytochemicals that encapsulate surface area of the nanoparticles. Preferred multicomponent nanomaterials combine physical and biological properties of multiple materials within a single nanoconstruct. Multicompone