US-12622435-B2 - Biocontrol formulation for controlling plant pathogens

US12622435B2US 12622435 B2US12622435 B2US 12622435B2US-12622435-B2

Abstract

A biocontrol formulation for plant pathogens includes a Trichoderma viride filtrate and an inert substance selected from the group consisting of a viscosity reducer, a surfactant, flour, clay, and combinations thereof. In an embodiment, the filtrate includes conidia of the Trichoderma viride . In an embodiment, the filtrate includes byproducts obtained from the fermentation of Trichoderma viride . In an embodiment, the byproducts include at least one of the organic acids, growth regulators, and amino acids.

Inventors

Abdulaziz Abdulrahman Alaskar
Fatimah Olyan Alotibi
Wesameldin Ismail Saber
Khaled Mohammed Ghoneim

Assignees

KING SAUD UNIVERSITY

Dates

Publication Date: 20260512
Application Date: 20251210

Claims (1)

1 . A method of making a biocontrol formulation for controlling plant pathogens consisting essentially of a Trichoderma viride filtrate derived from the fermentation of an endophytic Trichoderma viride isolate, wherein the filtrate consists essentially of Trichoderma viride conidia and growth regulators secreted by the Trichoderma viride during fermentation; and an inert substance selected from the group consisting of flour, kaolin, semolina durum, clay and combinations thereof; wherein the biocontrol formulation is formulated as a dried, shelf-stable granule.

Description

CROSS-REFERENCE TO RELATED APPLICATION This application is a divisional of U.S. patent application Ser. No. 19/296,043, filed on Aug. 11, 2025, a divisional of U.S. patent application Ser. No. 18/731,591, filed on Jun. 3, 2024, the entire contents of each of which are incorporated herein by reference. BACKGROUND 1. Field The present disclosure relates to biological fungicides and, more particularly, a biocontrol formulation including endophytic Trichoderma for combatting fungal pathogens and promoting plant growth. 2. Description of the Related Art Due to the harmful effects of chemical fungicides, pesticide-resistant strains of pathogens have become prevalent, as well as various health problems, such as cancer and chromosomal abnormalities in humans and animals. Accordingly, biological fungicides are preferable for use. Typically, however, the use of microorganisms for combatting plant pathogens tends to lose vitality and activity over time. Thus, a biocontrol formulation including endophytic Trichoderma for combatting fungal pathogens and promoting plant growth solving the aforementioned problems is desired. SUMMARY The present subject matter focuses on a biological formulation for plant growth, which includes a Trichoderma viride filtrate and an inert substance. In an embodiment, the filtrate includes conidia of the Trichoderma viride virus and fermentation byproducts of the fungus. In an embodiment, the fermentation byproducts are selected from the group consisting of organic acids, growth regulators, and amino acids. In an embodiment, the inert substance is selected from the group consisting of viscosity reducers and surfactants. In an embodiment, the inert substance is selected from the group consisting of flour, clay, and a mixture of flour and clay. The formulation can be stable during storage. For example, the formulation can be safely stored at temperatures ranging from about −5 to 35° C. In an embodiment, the biological formulations can be applied to plants to provide biological resistance against disease-causing agents in plants. In an embodiment, the biological formulation can be applied to plants to enhance plant growth, provide nutrients to the soil, especially in impoverished soils, and enhance the quality of plant products. These and other features of the present subject matter will become readily apparent upon further review of the following specification. BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a diagram showing how to produce the marketable bio-compound from. FIGS. 2A-2C are images depicting the mycelial radius of R. solani after an 8-day incubation period in the control (2A), the growth radius of T. viride (2B), and the growth of both R. solani and T. viride on the same plate (2C (Trichoderma envelops the disease-causing colony after 8 days of incubation in dual-culture plates). FIGS. 3A-3C are images illustrating the response of bean seeds to treatment with (3A) the control; (3B) the filtrate of the T. viride fungus; and (3) treatment with the fungicide, after 14 days of germination. FIGS. 4A-4B are images showing growth of (4A) Trichoderma viride; and (4B) and its biologically active granular composition. FIGS. 5A-5G are images showing the impact of the formula on plant growth under greenhouse, where (5A) represents (1) R. solani pathogen only; (5B) represents (2) pathogen+formula; (5C) represents (3) pathogen+recommended fungicide; (5D) represents (4) negative control without treatment; (5E) represents (5) individual application of the proposed formula composition without infection; (5F) represents (6) individual application of fungicide without infection; and (5G) shows increase in plant length of plants (1)-(6). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following definitions are provided for the purpose of understanding the present subject matter and for construing the appended patent claims. Definitions Throughout the application, where compositions are described as having, including, or comprising specific components, or where processes are described as having, including, or comprising specific process steps, it is contemplated that compositions of the present teachings can also consist essentially of, or consist of, the recited components and that the processes of the present teachings can also consist essentially of, or consist of, the recited process steps. It is noted that, as used in this specification and the appended claims, the singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise. In the application, where an element or component is said to be included in and/or selected from a list of recited elements or components, it should be understood that the element or component can be any one of the recited elements or components, or the element or component can be selected from a group consisting of two or more of the recited elements or components. Further, it should be understood that elements and/or features o