Search

US-12622445-B2 - Method for preparing tropical fish feed by using brine shrimp, and tropical fish feed prepared thereby

US12622445B2US 12622445 B2US12622445 B2US 12622445B2US-12622445-B2

Abstract

The present invention relates to a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, and, more specifically, to: a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, the method feeding spirulina and Haematococcus to hatched brine shrimp to prepare a tropical fish feed, and thus can promote the growth of tropical fish, boost the immune system and improve the consumption, digestion, absorption and palatability for tropical fish.

Inventors

  • Hong Sun SIM

Assignees

  • Hong Sun SIM

Dates

Publication Date
20260512
Application Date
20210902
Priority Date
20200902

Claims (7)

  1. 1 . A method of preparing a brine shrimp-based tropical fish feed, the method comprising: S 100 : preparing culture saline water; S 200 : adding a brine shrimp egg to the culture saline water of step S 100 to prepare to hatch the brine shrimp egg; S 300 : circulating air in the culture saline water of step S 200 to generate a fine air bubble in the culture saline water, thereby hatching the brine shrimp egg; S 400 : adding a first spirulina to the culture saline water of step S 300 after hatching of the brine shrimp egg; S 500 : separating and removing eggshell of the hatched brine shrimp egg from the culture saline water of step S 400 ; S 600 : adding culture saline water having the same concentration as the culture saline water of step S 100 to the culture saline water of S 500 ; S 700 : adding a second spirulina to the cultured saline water formed in step S 600 to supply nutrients to a hatched brine shrimp larva; S 800 : adding Haematococcus powder to the culture saline water of step S 700 ; S 900 : separating and removing additional eggshell from the culture saline water of step S 800 , and collecting the brine shrimp larva; S 1000 : freezing the collected brine shrimp larva, followed by re-separating and removing eggshell remaining in the frozen brine shrimp larva; and S 1100 , freezing and packaging the brine shrimp larva of step S 1000 to obtain the brine shrimp-based tropical fish feed, wherein in step S 400 , after 18 hours have elapsed since the brine shrimp egg starts to hatch, the first spirulina is added to the culture saline water at a weight ratio of 1.5-2.5 g spirulina/330-350 g brine shrimp egg; wherein in step S 700 , the second spirulina is added at an interval of 90-120 minutes until 48 hours have elapsed after 18 hours have elapsed after the brine shrimp egg hatches and the first spirulina is added so that a total of 90-100 g spirulina is added per 330-350 g of brine shrimp egg; wherein step S 800 comprises mixing a Haematococcus powder with water at a weight ratio of 2 g Haematococcus powder per 800 ml of water to form a mixture; placing the mixture in a grinder; pulverizing the mixture for 1 to 2 minutes, and adding the pulverized mixture to the culture saline water of step S 700 for 5 times at 2 g each from 40 hours to 50 hours after hatching of the brine shrimp egg; and wherein in step S 1000 , the collected brine shrimp larva is frozen for 2.5-3.5 hours and then introduced into fresh culture saline water to completely remove eggshell remaining from the hatched brine shrimp egg based on sinking of the frozen brine shrimp larva and floating of the eggshell.
  2. 2 . The method of claim 1 , wherein the culture saline water of step S 100 is prepared by dissolving 33-50 g salt in 1 liter of purified water having a temperature of 28-30° C., and wherein in step S 200 , prior to adding the brine shrimp egg to the culture saline water, a stock brine shrimp egg is refrigerated and stored at a temperature of −8 to −4° C., and the refrigerated and stored brine shrimp egg is then stored for 12 to 20 hours at a temperature of 15 to 25° C., and the brine shrimp egg is added to the culture saline water at a ratio of 330 g to 350 g of brine shrimp egg per 120 liters of culture saline water.
  3. 3 . The method of claim 2 , wherein in step S 300 , air is circulated at an amount of 6.5 to 7.5 L/min into the culture saline water to generate the fine air bubble, light with 2,000 to 2,500 lux is applied to the top and side of the culture saline water for up to 36 hours, and light with 1,000 lux is maintained until the brine shrimp egg hatches.
  4. 4 . The method of claim 3 , wherein in step S 900 , the culture saline water is passed through a pipe-shaped tube having a length of 1 to 1.5 meters, and circular magnets having a magnetic force of 10,000 to 12,000 Gauss are installed on both sides of the pipe-shaped tube to separate the eggshell passing through the pipe-shaped tube to separate and collect brine shrimp larva.
  5. 5 . A brine shrimp-based tropical fish feed prepared by the method of claim 1 .
  6. 6 . The method of claim 1 , wherein in step S 500 , after 40 hours have elapsed since hatching of the brine shrimp egg starts, air circulation in the culture saline water is stopped for 10 to 30 seconds and the eggshell of the hatched brine shrimp egg is separated using a bar magnet such that 70 to 80% of the eggshell of the hatched brine shrimp egg is removed.
  7. 7 . The method of claim 1 , wherein in step S 600 , 20 wt % of the culture saline water of step S 500 is first removed, and then 24 liters of culture saline water having a temperature of 26-28° C. is gradually added to the remaining 80% culture saline water.

Description

TECHNICAL FIELD The present invention relates to a method for preparing tropical fish feed using brine shrimp and a tropical fish feed prepared thereby, and more specifically, a method for preparing tropical fish feed using brine shrimp and a tropical fish feed prepared thereby that promotes the growth and strengthens immunity of tropical fish, strengthens immunity and improves intake, digestion, absorption and palatability of tropical fish. BACKGROUND ART Brine shrimp are small aquatic animals used as live feed in fish farming since ancient times and are still used today. Despite the recent development of various dry particulate feeds, the reason why live brine shrimp are in the limelight as fish food is that general users can easily purchase brine shrimp eggs as a product, the brine shrimp are easy to hatch, are small in size and have high nutritional value so that they are the best feeding organism for the health and growth of adult fish and fry. As a result, the demand is increasing for brine shrimp as feed for fish. The brine shrimp are also commonly referred to as Artemia, and the adult size is 10 to 15 mm, but their eggs and the hatched brine shrimp are less than 1 mm in size, so they are treated as the best feed for young fish with small mouths. However, brine shrimp eggs with unhatched shells and rotten eggs cause indigestion and ascites in young fish and adult fish, so young fish and adult fish are easily killed. Thus, special attention is required. All brine shrimp distributed for direct feeding are eggshell-exfoliated products from which eggshells have been removed or frozen forms immediately after hatching. For this reason, general users who raise fish can easily purchase brine shrimp eggs that have been dried and stored for hatching online and offline, and they directly hatch eggs using brine shrimp incubators or transparent PET bottles, remove shells and decayed eggs, and collect only hatched brine shrimp, and they are provided as feed for fish. A brief description of the generally used brine shrimp incubation method, collection device, and method according to the brine shrimp incubator is as follows. An outlet and a valve are combined at the bottom of a transparent container where the inner surface can be seen to some extent. The container is filled with clean water, salt is added and dissolved, an air pump is connected to supply air continuously, a certain amount of dried brine shrimp eggs is put in the saline water with an appropriate water temperature, and brine shrimp hatch after about 36 to 48 hours. The incubation proceeds for about 36 to 48 hours, and the brine shrimp wriggling after hatching are identified with the naked eye. When incubation progresses, and it is determined that brine shrimp can be collected, the air pump is turned off and waited for 20 to 30 minutes. The reason is that the water current is stable, the remaining hatched eggshells float on the surface of the water, the hatched brine shrimp move fluidly, and the unhatched eggs and decayed brine shrimp eggs are located in the middle and toward the bottom. Then, in order to use the characteristics of brine shrimp that gather toward the light, the surroundings of the container are made as dark as possible, and light is shined on the bottom of the transparent container so that brine shrimp gather around the light. This is using the instinctive characteristic of hatched brine shrimp that strongly gather toward the light emitting portion when they see the light. The methods for collecting brine shrimp gathered in this way includes attaching a valve to the bottom of the incubation container and collecting it by opening the valve, and directly collecting brine shrimp that are slightly clustered using a pipette or syringe. All of the above methods can collect hatched brine shrimp, but the collecting method by opening the valve may collect unhatched but decayed brine shrimp eggs, unhatched eggs, and some eggshells in addition to hatched eggs so it is fundamentally a problem that only hatched brine shrimp, which are the best feed conditions for fish, cannot be collected. In addition, there is a big problem that when rotten brine shrimp eggs and eggshells left after hatching are provided to other tanks as fish food, the water quality is rapidly polluted, and adult fish and fry eat them to induce indigestion and ascites, thereby causing death. Further, there are unavoidable problems that the direct collection method using a pipette or syringe has a small amount of hatched brine shrimp collected, and the sorting operation is cumbersome and practically difficult, so the brine shrimp hatched with a lot of time and effort have to be discarded along with the shells in addition to the above problems. Further, there are problems in the case of hatching brine shrimp eggs in small quantities. If brine shrimp are hatched just enough to be provided as feed, there is the inconvenience of repeating the incubation procedure, and repeated collect