US-12622453-B2 - Method for preparing water-soluble rutin powder and uses thereof

Abstract

A method for preparing a water-soluble rutin powder includes: heating and mixing a plant polysaccharide substance, a filler, and water to obtain a wall material solution; adding an antioxidant and a rutin powder into the wall material solution at a first temperature, followed by emulsification and dispersion, pH adjustment, and high-pressure homogenization to obtain a rutin powder emulsion; and carrying out heating fusion of the rutin powder emulsion to obtain a water-soluble rutin powder. With the preparation method, a water-soluble rutin product is obtained through emulsification, high-pressure homogenization, ultra-high-temperature fusion, drying and other processes by using modified starch and a plant polysaccharide as a wall material and rutin as a core material. The product features high rutin content, good water solubility, and high solution clarity, has good stability in an acidic solution environment, and can meet requirements of acidic transparent beverage for high clarity, high stability, and no precipitation.

Inventors

• Honglong LI
• Ziheng JIN
• Yanjun WEN
• Linzheng LI
• MINGMING WANG
• Yulian GUO
• Haitao Han
• Di Wang

Assignees

• HENAN ZHONGDA HENGYUAN BIOTECHNOLOGY STOCK CO., LTD.

Dates

Publication Date

20260512

Application Date

20231007

Priority Date

20221221

Claims (8)

1. 1 . A method for preparing a water-soluble rutin powder, comprising: step 1: heating and mixing a plant polysaccharide substance, a filler, and water to obtain a wall material solution; step 2: adding an antioxidant and a rutin powder into the wall material solution at a first temperature, followed by emulsification and dispersion, pH adjustment, and high-pressure homogenization to obtain a rutin powder emulsion; and step 3: carrying out heating fusion of the rutin powder emulsion to obtain the water-soluble rutin powder, wherein the heating and mixing are carried out at a temperature of 75° C. to 100° C.; the first temperature is 75° C. to 100° C.; the antioxidant is one or more selected from the group consisting of ascorbic acid, sodium erythorbate, epigallocatechin gallate (EGCG), and vitamin E; and a content in percentages by weight of the antioxidant in the water-soluble rutin powder is 0.01% to 1%.
2. 2 . The method according to claim 1 , wherein the plant polysaccharide substance is one or more selected from the group consigning of pullulan, fucoidan, modified starch, a plant-based gum, and dextrin; the modified starch is one or more selected from the group consisting of sodium octenylsuccinate starch, hydroxypropyl starch, acetate starch, carboxymethyl starch, phosphate starch, and microporous starch; the plant-based gum comprises one or more of acacia gum, ghatti gum, and xanthan gum; and the dextrin comprises resistant dextrin.
3. 3 . The method according to claim 1 , wherein the filler is one or more selected from the group of maltodextrin, microcrystalline cellulose, glucose syrup, white sugar, lactitol, erythritol, maltitol, sorbitol, α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, trehalose, xylooligosaccharide, maltooligosaccharide, lactose, and stachyose; the wall material solution is a colloidal solution; a mass concentration of the plant polysaccharide substance in the wall material solution is 5 wt % to 75 wt %; and a mass concentration of the filler in the wall material solution is 5 wt % to 75 wt %.
4. 4 . The method according to claim 1 , wherein the emulsification and dispersion are carried out at a temperature of 75° C. to 100° C.; the emulsification and dispersion are carried out at a rotational speed of 500 rpm to 20000 rpm; the emulsification and dispersion are carried out for 0.5 min to 60 min; and an adjusting agent for the pH adjustment comprises one or more of sodium carbonate, sodium bicarbonate, potassium carbonate, sodium hexametaphosphate, sodium tripolyphosphate, sodium polyphosphate, sodium pyrophosphate, and sodium citrate.
5. 5 . The method according to claim 1 , wherein in the pH adjustment, the pH of the wall material solution is 6.0 to 10.0; the high-pressure homogenization is carried out at a pressure of 0.1 MPa to 80 MPa; and the high-pressure homogenization is carried out for 1 to 5 times.
6. 6 . The method according to claim 1 , wherein the heating fusion is ultra-high-temperature fusion; the heating fusion is carried out at a temperature of 100° C. to 135° C.; the heating fusion is carried out at a pressure of 0 MPa to 0.5 MPa; and the heating fusion is carried out for 1 min to 60 min.
7. 7 . The method according to claim 1 , wherein the method further comprises a drying step after the heating fusion; the drying step comprises one or more of centrifugal spray drying, pressure spray drying, freeze drying, and hot air drying; wherein when the drying step comprises the centrifugal spray drying; the air inlet temperature in the centrifugal spray drying is 160° C. to 200° C.; and the air outlet temperature in the centrifugal spray drying is 70° C. to 100° C.
8. 8 . The method according to claim 1 , wherein the water-soluble rutin powder is of a structure having a wall material enveloping rutin powder particles; and the wall material is one or more selected from the group consisting of pullulan, fucoidan, modified starch, a plant-based gum, and dextrin.

Description

This application claims priority to Chinese Patent Application No. 202211648600.X, filed on Dec. 21, 2022, which is incorporated by reference for all purposes as if fully set forth herein. FIELD OF THE INVENTION The invention pertains to the technical field of rutin powder microencapsulation, and relates to a method for preparing a water-soluble rutin powder and uses thereof. DESCRIPTION OF THE RELATED ART Rutin, also known as rutoside or vitamin P, is a natural flavonoid glycoside widely present in plants. As a natural antioxidant, rutin can scavenge free radicals, inhibit lipid peroxidation, and inhibit platelet aggregation, and has anti-inflammatory, antioxidant, anti-allergic and, antiviral effects. Clinically, rutin is often used in the prevention and treatment of cerebral hemorrhage, hypertension, retinal hemorrhage, purpura, and acute hemorrhagic nephritis. Trihydroxyethylrutin (troxerutin), a derivative of rutin, is clinically used in the treatment of burns, arthritis, various vascular diseases including cerebrovascular diseases, retinal edema, hemorrhage, etc. Because of its good biological activity and high safety, rutin has been used as a raw material in healthy foods or functional foods. Sophora japonica, a main source of rutin, has also been used as a raw material in medicines and foods. Although rutin has good biological activity, its solubility in water is very low, and the rutin solution is prone to crystallization and precipitation in an acidic environment, leading to low absorption and bioavailability, greatly limiting the application of rutin. How to safely and effectively improve the water solubility of rutin and further improve the bioavailability of rutin has become the main direction of research on rutin. According to the published related literatures, the main research schemes on the application of rutin include liposome, micelle self-emulsification, copolymer complexation, microencapsulation in strong alkali environment, etc. For example, EP3082728 discloses a method for solubilizing rutin with polyhydroxyalkyl alcohols, CN201810525567.9 discloses a rutin-coated polymer micelle and preparation method thereof, EP3453766 discloses a method for producing flavonoid inclusion compounds, CN113304113 discloses a co-amorphous solid dispersion for improving dissolution of quercetin and preparation method thereof, and CN114271492 discloses a raw material composition of high-content rutin powder, rutin powder, and preparation method and application thereof. Although the improved methods can improve the dissolution of rutin to a certain extent, most of the preparation processes are complex and require a large amount of chemically synthesized surfactant or the use of a strong alkaline solution, leading to the problems of high costs, complex process, low safety, poor product stability, low solution transparency, small pH tolerance range, and so on. Therefore, how to find a more suitable modification method that can solve the problems in the existing technologies while safely and effectively improving the water solubility of rutin has become one of the urgent problems to be solved by front-line researchers and R&D enterprises. SUMMARY OF THE INVENTION Accordingly, the technical problem to be solved by the invention is to provide a method for preparing a water-soluble rutin powder and use of the water-soluble rutin powder. The method provided by the invention is a preparation method for obtaining a water-soluble rutin powder product with high rutin content without using any surfactant or any strong alkaline solution. The product of the invention features high rutin content, good water solubility, high solution clarity, and good stability in low pH solution environment, and particularly can meet the requirements of acidic transparent beverage for high clarity, high stability, and no precipitation. The invention provides a method for preparing a water-soluble rutin powder, which includes: step 1: heating and mixing a plant polysaccharide substance, a filler, and water to obtain a wall material solution;step 2: adding an antioxidant and a rutin powder into the wall material solution at a first temperature, followed by emulsification and dispersion, pH adjustment, and high-pressure homogenization to obtain a rutin powder emulsion; andstep 3: carrying out heating fusion of the rutin powder emulsion to obtain a water-soluble rutin powder. Preferably, the plant polysaccharide substance includes one or more of pullulan, fucoidan, modified starch, a plant-based gum, and dextrin; the modified starch includes one or more of sodium octenylsuccinate starch, hydroxypropyl starch, acetate starch, carboxymethyl starch, phosphate starch, and microporous starch;the plant-based gum includes one or more of acacia gum, ghatti gum, and xanthan gum; andthe dextrin includes resistant dextrin. Preferably, the filler includes one or more of maltodextrin, microcrystalline cellulose, glucose syrup, white sugar, lactitol, eryt