US-12622941-B2 - Anticoccidial composition comprising ginkgo leaves and use thereof

Abstract

The present application relates to an anticoccidial composition comprising ginkgo leaves and use thereof. The composition comprising ginkgo leaves, according to an embodiment, has excellent effects of directly killing sporozoites that can induce coccidiosis, inhibiting the penetration of the sporozoites into cells, and/or inhibiting the proliferation of the sporozoites in cells, and has excellent effects of preventing, ameliorating and treating coccidiosis in vivo.

Inventors

• Kyuyeol Son
• Min Ah PARK
• Kyung Min Lee
• Hwi-Jea Kim

Assignees

• CJ CHEILJEDANG CORPORATION

Dates

Publication Date

20260512

Application Date

20210924

Priority Date

20200925

Claims (18)

1. 1 . A method for alleviating or treating coccidiosis in an animal in need thereof, comprising administering a composition comprising a ginkgo leaf, or at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Bilobalide, and a pharmaceutically acceptable salt thereof as an active ingredient, to the animal.
2. 2 . The method according to claim 1 , wherein the coccidiosis is induced by an Eimeria sp. protozoan.
3. 3 . The method according to claim 1 , wherein the alleviating or treating coccidiosis is at least one selected from the group consisting of the following (1) to (4): (1) reduction of at least one selected from the group consisting of lesion score, fecal oocyst excretion amount and mortality; (2) inhibition of weight loss due to coccidiosis; (3) increase of an anticoccidial index (ACI); and (4) reduction of cell invasion of an Eimeria sp. protozoan, propagation of the protozoan in cells, or both of them.
4. 4 . The method according to claim 1 , wherein the ginkgo leaf comprises at least one selected from the group consisting of raw material, dried material, pulverized material and extract of the ginkgo leaf.
5. 5 . The method according to claim 4 , wherein the ginkgo leaf extract is extracted by a solvent extraction method using an extraction solvent selected from the group consisting of water, linear or branched chain alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, hexane and mixtures thereof.
6. 6 . The method according to claim 1 , wherein the composition is a feed composition comprising the ginkgo leaf or the active ingredient at a concentration of 1% (w/w) or less based on the total weight.
7. 7 . The method according to claim 1 , wherein the composition is a feed additive.
8. 8 . The method according to claim 1 , wherein the composition further comprises at least one selected from the group consisting of Quercetin, Kaempferol, and a pharmaceutically acceptable salt thereof as an active ingredient.
9. 9 . The method according to claim 1 , wherein the composition excludes at least one selected from the group consisting of Quercetin, Kaempferol, and a salt thereof.
10. 10 . The method according to claim 1 , wherein the method treats coccidiosis in the animal.
11. 11 . The method according to claim 1 , wherein the ginkgo leaf extract is not extracted by a solvent extraction method using an extraction solvent comprising a linear chain alcohol having 1 to 4 carbon atoms.
12. 12 . A method for killing Eimeria sp. protozoan, or inhibiting cell invasion or propagation of Eimeria sp. Protozoan in an animal in need thereof, comprising administering a composition, comprising a ginkgo leaf, or at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Bilobalide, and a salt thereof as an active ingredient, to the animal.
13. 13 . The method according to claim 12 , wherein the ginkgo leaf comprises at least one selected from the group consisting of raw material, dried material, pulverized material and extract of the ginkgo leaf.
14. 14 . The method according to claim 13 , wherein the ginkgo leaf extract is extracted by a solvent extraction method using an extraction solvent selected from the group consisting of water, linear or branched chain alcohols having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, hexane and mixtures thereof.
15. 15 . The method according to claim 12 , wherein the composition further comprises at least one selected from the group consisting of Quercetin, Kaempferol, and a salt thereof as an active ingredient.
16. 16 . The method according to claim 12 , wherein the composition excludes at least one selected from the group consisting of Quercetin, Kaempferol, and a salt thereof.
17. 17 . The method according to claim 1 , wherein the method kills Eimeria sp. Protozoan in the animal.
18. 18 . The method according to claim 12 , wherein the ginkgo leaf extract is not extracted by a solvent extraction method using an extraction solvent comprising a linear chain alcohol having 1 to 4 carbon atoms.

Description

A computer readable text file, entitled “SequenceListing.txt,” created on or about Feb. 15, 2023 with a file size of 711 bytes contains the sequence listing for this application and is hereby incorporated by reference in its entirety. CROSS-REFERENCE TO RELATED APPLICATION(S) Technical Field The present application claims the benefit of the priority based on Korean Patent Application No. 10-2020-0125244 filed on Sep. 25, 2020, and the entire contents disclosed in the document of the corresponding Korean patent application are incorporated as a part of the present description. The present application relates to an anticoccidial composition comprising a ginkgo leaf and uses thereof. Background Art Coccidiosis is an intestinal-related disease caused by a protozoan parasite belonging to the phylum apicomplexan called Eimeria, and when affected with coccidiosis, symptoms of digestive disorders, diarrhea and weight loss are shown, and furthermore, death of livestock is caused, and therefore, it has a major economic impact on farms around the world (Williams R B. A compartmentalised model for the estimation of the cost of coccidiosis to the world's chicken production industry. Int J Parasitol. 1999; 29(8):1209-1229). Over the past few years, many researchers have developed anticoccidial agents such as ionophores or chemically synthetic compounds, which can prevent oocyst cell wall formation or asexual and sexual reproduction of protozoa as therapeutic agents for treating coccidiosis. However, side effects such as appearance of protozoa with drug resistance due to the long-term use of a shuttle program that alternately treats the ionophores and chemically synthetic compounds and the like occurred. In particular, antibiotics accumulated in animals due to misuse and abuse of antibiotics are a serious problem as humans consume antibiotics through meat, and therefore, antibiotic administration are being banned in many countries around the world due to the problem of antibiotic residues in livestock products. Therefore, there is an urgent need to develop and research alternatives to conventional anticoccidial agents that exhibit side effects such as emergence and internal residue of strains resistant to drugs. PRIOR ART Patent Document (Patent document 1) U.S. Patent Publication No. 2008-0160000 DISCLOSURE Technical Problem One embodiment of the present application provides a feed composition for preventing or alleviating coccidiosis, comprising at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and salt thereof as an active ingredient. Another embodiment of the present application provides a feed composition for preventing or alleviating coccidiosis, comprising a ginkgo leaf. Other embodiment of the present application provides a pharmaceutical composition for preventing or treating coccidiosis, comprising at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and pharmaceutically acceptable salt thereof as an active ingredient. Other embodiment of the present application provides a pharmaceutical composition for preventing or treating coccidiosis, comprising a ginkgo leaf. Other embodiment of the present application provides an antiprotozoal composition against an Eimeria sp. protozoa, comprising at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and salt thereof as an active ingredient. Other embodiment of the present application provides an antiprotozoal composition against an Eimeria sp. protozoa, comprising a ginkgo leaf. Other embodiment of the present application provides a method for preventing, alleviating or treating coccidiosis, comprising a step of administering the composition to an animal except for human. Other embodiment of the present application provides a use of at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and salt thereof, for the manufacture of a composition (for example, feed composition, pharmaceutical composition) for preventing, alleviating and/or treating coccidiosis or an antiprotozoal composition; a use of at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and salt thereof, for using in preventing, alleviating and/or treating coccidiosis; and/or a use of at least one selected from the group consisting of Ginkgolide A, Ginkgolide B, Ginkgolide C, Quercetin, Kaempferol, Bilobalide, and salt thereof, for using in antiprotozoan against an Eimeria sp. protozoan (for example, Eimeria sp. protozoa killing; and/or cell invasion and/or propagation inhibition of Eimeria sp. protozoa). Other embodiment of the present application provides a use of a ginkgo leaf for the manufacture of a composition (for example, feed composi