US-12623000-B2 - Composition for transplantation of organoid

Abstract

The present invention relates to a composition for transplantation comprising an organoid, and a use of same. According to one example, using collagen, gelatin or fibrin glue as a scaffold for organoid transplantation results in a high transplantation rate and a high survival rate of organoid as well as desirable stability.

Inventors

• Jong Man YOO
• Joo Hyun JEE
• Sang Yun Jung
• Han Kyung Kim
• Joong Woon LEE

Assignees

• ORGANOIDSCIENCES, LTD.

Dates

Publication Date

20260512

Application Date

20191015

Priority Date

20181130

Claims (3)

1. 1 . A composition for biotransplantation consisting of organoid and fibrin glue, wherein the fibrin glue is comprised in an amount of 10 to 15% (v/v) based on the total weight of the composition.
2. 2 . The composition for biotransplantation according to claim 1 , wherein the organoid is selected from the group consisting of intestinal organoid, retinal organoid, kidney organoid, liver organoid, gastric organoid, prostate organoid, breast organoid, inner ear organoid, cardiac muscle fiber organoid, hepatic endothelial organoid, pancreatic organoids, fallopian tube organoids, and cerebral organoids.
3. 3 . A method for transplanting an organoid, the method comprising mixing fibrin glue with the organoid to prepare the composition according to claim 1 ; and administering the composition to a subject.

Description

CROSS REFERENCE TO RELATED APPLICATIONS This application is a National Stage of International Application No. PCT/KR2019/013527, filed Oct. 15, 2019, claiming priority to Korean Patent Application No. 10-2018-0152804, filed Nov. 30, 2018, the entire disclosures of which are incorporated herein by reference. TECHNICAL FIELD The present invention relates to a composition for transplantation of an organoid, and a use of same. BACKGROUND ART Organoids are considered basic experimental models, sources of implantable tissue, and physiologically relevant platforms for drug screening. In contrast to a culture of immortalized cells, intestinal organoids, for example, contain viable stem cells residing in crypt-like lobes, undergo a continuous cycle of regeneration and differentiation to produce multiple functional cell types and repeat key aspects of gut development and homeostasis. Epithelial organoids can be formed from human colon, adenoma, and adenocarcinoma tissue and cultured from personalized medicine, patient-derived crypt or ex vivo and opened up possibilities for autologous transplantation using stem cells culture and proliferated in ex vivo. Despite a histological accuracy of the original tissues, stem cell-derived organoids from the gastrointestinal tract (GI tract) have several limitations, a main one of which is depending on Matrigel as a 3D scaffold. Matrigel is a widely used commercial product to provide a 3D scaffold for the growth of organoids of all cell types. It is used to grow intestine, retina, kidney, liver, stomach, prostate, breast, inner ear, cardiac muscle fibers, hepatic endothelium, pancreas, fallopian tubes, and cerebral organoids. It is also used for growing organoids from a variety of species including chickens, rats and humans. However, reliance on Matrigel or similar naturally derived biopolymer matrices as scaffolds for organoid growth poses several significant limitations to the study and use of the resulting organoids. Matrigel is derived from basement membrane of ECM-rich mouse sarcoma and therefore carries a high risk of transmitting immunogens or pathogens when given to a patient, particularly problematic in the field of serious patient death and morbidity related to infection following immunosuppression and it is known to promote angiogenesis and cancer development. In addition, the batch-to-batch variability of Matrigel can lead to inconsistent cell behavior introducing unknown and potentially confounding variables that complicate the interpretation of both basic and translational research. Moreover, although Matrigel is a crucial element of the current organoid culture model, its role in organoid formation has not been elucidated. Therefore, there is a need for research on a scaffold for human injection which is effective enough to replace a matrigel in the field of organoids and suitable for transplantation in a body. DESCRIPTION OF THE INVENTION Technical Problem One aspect of the present invention is to provide a composition for biotransplantation comprising an organoid, and a gelatin, collagen, fibrin glue, or a combination of thereof. Another aspect of the present invention is to provide a method for organoid transplantation comprising a step of mixing gelatin, collagen, fibrin glue, or a combination thereof with an organoid and a step of administering the mixture to a subject. Technical Solution One aspect of the present invention provides a composition for biotransplantation comprising organoid, and a gelatin, collagen, fibrin glue, or a combination thereof. The term “organoid” as used herein refers to a cell mass having a 3D three-dimensional structure and refers to a miniaturized and simplified version of an organ prepared through an artificial culture process that is not collected or acquired from animals. The origin of the cells constituting the organoid is not limited. Organoids can be derived from tissues, stem cells, for example, embryonic stem cells or induced pluripotent stem cells, and may be cultured in three dimensions from their self-renewal and differentiation ability. The organoid may have an environment that is allowed to interact with the surrounding environment during the cell growth process. Accordingly, the 3D organoid in the present invention almost completely mimics the organs that interact in vivo and can be an excellent model for observing the development of therapeutic agents for diseases. It can similarly reproduce the physiologically active function of the human body, and by constructing an organ analogue from the patient's tissue, disease modeling based on the patient's genetic information and drug screening through repeated tests are possible. For this function, it is required to have excellent transplantation rate, engraftment ratio, survival rate and stability when transplanted into a living body. The term “gelatin” as used herein refers to a kind of a protein obtained by decomposing and purifying natural proteins composed in animal sk