US-12623214-B2 - Gene amplification chip, apparatus for gene amplification, and apparatus for bio-particle analysis

US12623214B2US 12623214 B2US12623214 B2US 12623214B2US-12623214-B2

Abstract

A gene amplification chip may include: a cover layer having a solution inlet through which a sample solution to be injected; a chamber layer disposed on one surface of the cover layer, and having a chamber to receive the sample solution when the sample solution is injected through the solution inlet such that an amplification reaction of the sample solution occurs in the chamber; a bottom layer disposed on another surface of the chamber layer; and a photothermal film attached to an outer surface of the bottom layer, and configured to convert light into heat to heat the sample solution received in the chamber.

Inventors

Jae Hong Lee
Kak Namkoong
Hyeong Seok Jang
Won Jong JUNG
Hyung Jun Youn

Assignees

SAMSUNG ELECTRONICS CO., LTD.

Dates

Publication Date: 20260512
Application Date: 20220928
Priority Date: 20220527

Claims (20)

1 . A gene amplification chip comprising: a cover layer having a solution inlet through which a sample solution to be injected; a chamber layer disposed on one surface of the cover layer, and having a chamber configured to receive the sample solution when the sample solution is injected through the solution inlet such that an amplification reaction of the sample solution occurs in the chamber; a bottom layer disposed on another surface of the chamber layer; and a photothermal film having a planar shape attached to an outer surface of the bottom layer, and configured to convert light into heat to heat the sample solution received in the chamber.
2 . The gene amplification chip of claim 1 , wherein the photothermal film is formed as a flat surface and is attached to all or a portion of the outer surface of the bottom layer.
3 . The gene amplification chip of claim 1 , wherein the photothermal film has a thickness of 500 μm or less.
4 . The gene amplification chip of claim 1 , wherein the photothermal film is formed of at least one of polymer, metal, metal oxide, nanocomposite, nanostructure, and semiconductor.
5 . The gene amplification chip of claim 1 , wherein the cover layer comprises at least one of silicon, metal, glass, and polymer.
6 . The gene amplification chip of claim 1 , wherein the chamber layer and the bottom layer are integrally formed using at least one of silicon, metal, and polymer, or are separately formed using different materials.
7 . The gene amplification chip of claim 1 , wherein a sum of a thickness of the chamber layer and a thickness of the bottom layer is 1 mm or less.
8 . The gene amplification chip of claim 1 , wherein the chamber has a single space to receive the sample solution therein, and the single space has a volume of 10 μL or less.
9 . The gene amplification chip of claim 1 , wherein the chamber has a plurality of through holes in which the sample solution is to be filled, and each of the plurality of through holes has a volume of at least 100 μL or higher.
10 . The gene amplification chip of claim 9 , wherein at least one of the plurality of through holes has a circular or polygonal prism shape and is formed to pass through the gene amplification chip in a direction from the cover layer toward the bottom layer.
11 . The gene amplification chip of claim 9 , wherein the cover layer further comprises a first channel that allows the sample solution to flow into the plurality of through holes, and a solution outlet configured to discharge the sample solution that remains in the first channel.
12 . The gene amplification chip of claim 11 , wherein the bottom layer comprises an oil inlet through which oil is to be injected, and a second channel configured to contain the oil.
13 . The gene amplification chip of claim 1 , wherein the cover layer, the chamber layer, and the bottom layer are formed separately, or at least two successive layers of wherein the cover layer, the chamber layer, and the bottom layer are integrally formed.
14 . An apparatus comprising the gene amplification chip of claim 1 , wherein the apparatus further comprises: a light source configured to emit the light onto the gene amplification chip to heat the sample solution, to cause the amplification reaction to occur in the chamber.
15 . The apparatus of claim 14 , further comprising a temperature sensor disposed on a surface of the photothermal film or at a portion of the outer surface of the bottom layer, at which the photothermal film is not disposed, and configured to measure temperature of the photothermal film or the bottom layer.
16 . The apparatus of claim 15 , wherein the temperature sensor comprises at least one of an infrared sensor or a thermocouple.
17 . The apparatus of claim 15 , further comprising a light source controller configured to control at least one of on and off, a light intensity, a light emission time, and a light emission period of the light source, based on the temperature measured by the temperature sensor.
18 . An apparatus for bio-particle analysis, the apparatus comprising: a gene amplification chip configured to perform gene amplification on a sample solution; a light source configured to emit light onto the gene amplification chip to heat the sample solution, to allow the gene amplification to occur in the gene amplification chip; a detector configured to detect a signal generated in response to occurrence of the gene amplification of the sample solution; and a processor configured to analyze bio-particles based on the detected signal, wherein the gene amplification chip comprises: a cover layer having a solution inlet through which the sample solution is to be injected; a chamber layer disposed on one surface of the cover layer and having a chamber to receive the sample solution when the sample solution is injected through the solution inlet such that an amplification reaction of the solution occurs in the chamber; a bottom layer disposed on another surface of the chamber layer; and a photothermal film having a planar shape attached to an outer surface of the bottom layer, and configured to convert the light that is received from the light source, into the heat to heat the sample solution received in the chamber.
19 . The apparatus of claim 18 , further comprising: a temperature sensor disposed on a surface of the photothermal film or at a portion of an outer surface of the chamber layer, at which the photothermal film is not disposed, and configured to measure temperature of the photothermal film or the chamber layer; and a light source controller configured to control at least one of on and off, a light intensity, a light emission time, and a light emission period of the light source, based on the temperature measured by the temperature sensor.
20 . A non-transitory computer readable storage medium which is, when executed by at least on processor, configured to perform a method of controlling an apparatus for bio-particle analysis, the method comprising: controlling a gene amplification chip to perform gene amplification on a sample solution, wherein a photothermal film having a planar shape is attached to the gene amplification chip; controlling a light source to emit light onto the photothermal film of the gene amplification to cause the photothermal film to convert the light to heat and thereby to heat the sample solution; detecting a signal generated from the gene amplification chip in response to the gene amplification of the sample solution occurring by the heat; and analyzing bio-particles based on the detected signal.

Description

CROSS-REFERENCE TO RELATED APPLICATION(S) This application claims priority from Korean Patent Application No. 10-2022-0065218, filed on May 27, 2022, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein by reference in their entireties. BACKGROUND 1. Field Apparatuses and methods consistent with example embodiments relate to a gene amplification chip, an apparatus for gene amplification, and an apparatus for bio-particle analysis. 2. Description of the Related Art Clinical or environmental samples are analyzed by a series of biochemical, chemical, and mechanical treatment processes. Recently, there has been much interest in developing techniques for diagnosis or monitoring of biological samples. Molecular diagnostic methods based on nucleic acid amplification techniques have excellent accuracy and sensitivity, and thus are increasingly used in various applications, ranging from diagnosis of infectious diseases or cancer to pharmacogenomics, development of new drugs, and the like. The polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used as a core technology in molecular biological diagnostic methods. The PCR is a method of detecting a target nucleic acid by amplifying a specific nucleic acid sequence, in which heating and cooling processes are repeated to activate or inhibit an enzyme for copying nucleic acid sequences. By gene amplification, the PCR can detect the presence of a target DNA sequence in a sample, and research is conducted for simplifying the PCR process to reduce time and provide user convenience, and manufacturing a PCR system in a compact size for application in point-of-care testing or personal testing. SUMMARY According to an aspect of the present disclosure, a gene amplification chip may include: a cover layer having a solution inlet through which a sample solution to be injected; a chamber layer disposed on one surface of the cover layer, and having a chamber configured to receive the sample solution when the sample solution is injected through the solution inlet such that an amplification reaction of the sample solution occurs in the chamber; a bottom layer disposed on another surface of the chamber layer; and a photothermal film attached to an outer surface of the bottom layer, and configured to convert light into heat to heat the sample solution received in the chamber. The photothermal film may be formed as a flat surface and is attached to all or a portion of the outer surface of the bottom layer. The photothermal film may have a thickness of 500 μm or less. The photothermal film may be formed of at least one of polymer, metal, metal oxide, nanocomposite, nanostructure, and semiconductor. The cover layer may include at least one of silicon, metal, glass, and polymer. The chamber layer and the bottom layer may be integrally formed using at least one of silicon, metal, and polymer, or are separately formed using different materials. A sum of a thicknesses of the chamber layer and a thicknesses the bottom layer may be 1 mm or less. The chamber may have a single space to receive the sample solution therein, and the single space may have a volume of 10 μL or less. The chamber may have a plurality of through holes in which the sample solution is to be filled, and each of the plurality of through holes may have a volume of at least 100 pL or higher. At least one of the plurality of through holes may have a circular or polygonal prism shape and may be formed to pass through the gene amplification chip in a direction from the cover layer toward the bottom layer. The cover layer may further include a first channel that allows the sample solution to flow into the plurality of through holes, and a solution outlet configured to discharge the sample solution that remains in the first channel, is discharged. The bottom layer may include an oil inlet through which oil is to be injected, and a second channel configured to contain the oil. The cover layer, the chamber layer, and the bottom layer are formed separately, or at least two successive layers of wherein the cover layer, the chamber layer, and the bottom layer are integrally formed. An apparatus including the gene amplification chip may include: a light source configured to emit the light onto the gene amplification chip to heat the sample solution, to cause the amplification reaction to occur in the chamber. The apparatus may further include a temperature sensor disposed on a surface of the photothermal film or at a portion of the outer surface of the bottom layer, at which the photothermal film is not disposed, and configured to measure temperature of the photothermal film or the bottom layer. The temperature sensor may include at least one of an infrared sensor or a thermocouple. The apparatus may include a light source controller configured to control at least one of on and off, a light intensity, a light emission time, and a light emission period o