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US-12624105-B2 - Chimeric antigen and t cell receptors and methods of use

US12624105B2US 12624105 B2US12624105 B2US 12624105B2US-12624105-B2

Abstract

Provided is a chimeric antigen receptor (CAR) or a T cell receptor (TCR) comprising one or more of the antigen binding motifs disclosed herein. Aspects of the disclosure relate to a polynucleotide encoding a chimeric antigen receptor (CAR) or a T cell receptor (TCR) comprising one or more of the antigen binding motifs. Provided are antibodies and antigen binding systems that comprise a binding motif that binds CD20 and optionally a binding motif that binds CD19, and methods of producing and using the same. Antibodies and antigen binding systems of the present disclosure comprise CARs that comprise an anti-CD20 binding motif and an anti-CD19 binding motif. Provided are compositions, such as antibodies and CARs that are or comprise an anti-CD20/anti-CD19 antigen binding system of the present disclosure, and cell therapies comprising the same, are useful, e.g., in the treatment of cancer.

Inventors

  • Arianne Perez
  • Stuart A. Sievers
  • Ruben Alvarez Rodriguez
  • Jonathan Belk
  • Jed WILTZIUS

Assignees

  • KITE PHARMA, INC.

Dates

Publication Date
20260512
Application Date
20230920

Claims (19)

  1. 1 . A method for treating a B cell cancer, comprising administering to the subject cells expressing a chimeric antigen receptor comprising an antibody or antigen binding fragment thereof having binding specificity to CD20, wherein the antibody or antigen binding fragment thereof comprises a heavy chain variable domain (VH) comprising complementarity determining regions HCDR1, HCDR2 and HCDR3, and a light chain variable domain (VL) comprising complementarity determining regions LCDR1, LCDR2 and LCDR3, wherein: (i) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 3-5; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 6-8; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 9-11; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 14-16; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 17-19; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 20-22; (ii) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 25-27; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 28-30; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 31-33; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 36-38; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 39-41; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 42-44; (iii) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 47-49; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 50-52; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-55; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 58-60; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 61-63; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 64-66; (iv) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 69-71; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 72-74; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 75-77; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 80-82; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 83-85; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 86-88; (v) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 91-93; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 94-96; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 97-99; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 102-104; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 105-107; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 108-110; (vi) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 113-115; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 116-118; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 119-121; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 124-126; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 127-129; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 130-132; (vii) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 135-137; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 138-140; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 141-143; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 146-148; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 149-151; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 152-154; (viii) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 157-159; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 160-162; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 163-165; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 168-170; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 171-173; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 174-176; (ix) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 179-181; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 182-184; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 185-187; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 190-192; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 193-195; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 196-198; or (x) the HCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 201-203; the HCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 204-206; the HCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 207-209; the LCDR1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 212-214; the LCDR2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 215-217; and the LCDR3 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 218-220.
  2. 2 . The method of claim 1 , wherein: (i) the HCDR1 comprises the amino acid of SEQ ID NO: 3; the HCDR2 comprises the amino acid of SEQ ID NO: 6; the HCDR3 comprises the amino acid of SEQ ID NO: 9; the LCDR1 comprises the amino acid of SEQ ID NO: 14; the LCDR2 comprises the amino acid of SEQ ID NO: 17; and the LCDR3 comprises the amino acid of SEQ ID NO: 20; (ii) the HCDR1 comprises the amino acid of SEQ ID NO: 25; the HCDR2 comprises the amino acid of SEQ ID NO: 28; the HCDR3 comprises the amino acid of SEQ ID NO: 31; the LCDR1 comprises the amino acid of SEQ ID NO: 36; the LCDR2 comprises the amino acid of SEQ ID NO: 39; and the LCDR3 comprises the amino acid of SEQ ID NO: 42; (iii) the HCDR1 comprises the amino acid of SEQ ID NO: 47; the HCDR2 comprises the amino acid of SEQ ID NO: 50; the HCDR3 comprises the amino acid of SEQ ID NO: 53; the LCDR1 comprises the amino acid of SEQ ID NO: 58; the LCDR2 comprises the amino acid of SEQ ID NO: 61; and the LCDR3 comprises the amino acid of SEQ ID NO: 64; (iv) the HCDR1 comprises the amino acid of SEQ ID NO: 69; the HCDR2 comprises the amino acid of SEQ ID NO: 72; the HCDR3 comprises the amino acid of SEQ ID NO: 75; the LCDR1 comprises the amino acid of SEQ ID NO: 80; the LCDR2 comprises the amino acid of SEQ ID NO: 83; and the LCDR3 comprises the amino acid of SEQ ID NO: 86; (v) the HCDR1 comprises the amino acid of SEQ ID NO: 91; the HCDR2 comprises the amino acid of SEQ ID NO: 94; the HCDR3 comprises the amino acid of SEQ ID NO: 97; the LCDR1 comprises the amino acid of SEQ ID NO: 102; the LCDR2 comprises the amino acid of SEQ ID NO: 105; and the LCDR3 comprises the amino acid of SEQ ID NO: 108; (vi) the HCDR1 comprises the amino acid of SEQ ID NO: 113; the HCDR2 comprises the amino acid of SEQ ID NO: 116; the HCDR3 comprises the amino acid of SEQ ID NO: 119; the LCDR1 comprises the amino acid of SEQ ID NO: 124; the LCDR2 comprises the amino acid of SEQ ID NO: 127; and the LCDR3 comprises the amino acid of SEQ ID NO: 130; (vii) the HCDR1 comprises the amino acid of SEQ ID NO: 135; the HCDR2 comprises the amino acid of SEQ ID NO: 138; the HCDR3 comprises the amino acid of SEQ ID NO: 141; the LCDR1 comprises the amino acid of SEQ ID NO: 146; the LCDR2 comprises the amino acid of SEQ ID NO: 149; and the LCDR3 comprises the amino acid of SEQ ID NO: 152; (viii) the HCDR1 comprises the amino acid of SEQ ID NO: 157; the HCDR2 comprises the amino acid of SEQ ID NO: 160; the HCDR3 comprises the amino acid of SEQ ID NO: 163; the LCDR1 comprises the amino acid of SEQ ID NO: 168; the LCDR2 comprises the amino acid of SEQ ID NO: 171; and the LCDR3 comprises the amino acid of SEQ ID NO: 174; (ix) the HCDR1 comprises the amino acid of SEQ ID NO: 179; the HCDR2 comprises the amino acid of SEQ ID NO: 182; the HCDR3 comprises the amino acid of SEQ ID NO: 185; the LCDR1 comprises the amino acid of SEQ ID NO: 190; the LCDR2 comprises the amino acid of SEQ ID NO: 193; and the LCDR3 comprises the amino acid of SEQ ID NO: 196; or (x) the HCDR1 comprises the amino acid of SEQ ID NO: 201; the HCDR2 comprises the amino acid of SEQ ID NO: 204; the HCDR3 comprises the amino acid of SEQ ID NO: 207; the LCDR1 comprises the amino acid of SEQ ID NO: 212; the LCDR2 comprises the amino acid of SEQ ID NO: 215; and the LCDR3 comprises the amino acid of SEQ ID NO: 218.
  3. 3 . The method of claim 1 , wherein: (i) the HCDR1 comprises the amino acid of SEQ ID NO: 4; the HCDR2 comprises the amino acid of SEQ ID NO: 7; the HCDR3 comprises the amino acid of SEQ ID NO: 10; the LCDR1 comprises the amino acid of SEQ ID NO: 15; the LCDR2 comprises the amino acid of SEQ ID NO: 18; and the LCDR3 comprises the amino acid of SEQ ID NO: 21; (ii) the HCDR1 comprises the amino acid of SEQ ID NO: 26; the HCDR2 comprises the amino acid of SEQ ID NO: 29; the HCDR3 comprises the amino acid of SEQ ID NO: 32; the LCDR1 comprises the amino acid of SEQ ID NO: 37; the LCDR2 comprises the amino acid of SEQ ID NO: 40; and the LCDR3 comprises the amino acid of SEQ ID NO: 43; (iii) the HCDR1 comprises the amino acid of SEQ ID NO: 48; the HCDR2 comprises the amino acid of SEQ ID NO: 51; the HCDR3 comprises the amino acid of SEQ ID NO: 54; the LCDR1 comprises the amino acid of SEQ ID NO: 59; the LCDR2 comprises the amino acid of SEQ ID NO: 62; and the LCDR3 comprises the amino acid of SEQ ID NO: 65; (iv) the HCDR1 comprises the amino acid of SEQ ID NO: 70; the HCDR2 comprises the amino acid of SEQ ID NO: 73; the HCDR3 comprises the amino acid of SEQ ID NO: 76; the LCDR1 comprises the amino acid of SEQ ID NO: 81; the LCDR2 comprises the amino acid of SEQ ID NO: 84; and the LCDR3 comprises the amino acid of SEQ ID NO: 87; (v) the HCDR1 comprises the amino acid of SEQ ID NO: 92; the HCDR2 comprises the amino acid of SEQ ID NO: 95; the HCDR3 comprises the amino acid of SEQ ID NO: 98; the LCDR1 comprises the amino acid of SEQ ID NO: 103; the LCDR2 comprises the amino acid of SEQ ID NO: 106; and the LCDR3 comprises the amino acid of SEQ ID NO: 109; (vi) the HCDR1 comprises the amino acid of SEQ ID NO: 114; the HCDR2 comprises the amino acid of SEQ ID NO: 117; the HCDR3 comprises the amino acid of SEQ ID NO: 120; the LCDR1 comprises the amino acid of SEQ ID NO: 125; the LCDR2 comprises the amino acid of SEQ ID NO: 128; and the LCDR3 comprises the amino acid of SEQ ID NO: 131; (vii) the HCDR1 comprises the amino acid of SEQ ID NO: 136; the HCDR2 comprises the amino acid of SEQ ID NO: 139; the HCDR3 comprises the amino acid of SEQ ID NO: 142; the LCDR1 comprises the amino acid of SEQ ID NO: 147; the LCDR2 comprises the amino acid of SEQ ID NO: 150; and the LCDR3 comprises the amino acid of SEQ ID NO: 153; (viii) the HCDR1 comprises the amino acid of SEQ ID NO: 158; the HCDR2 comprises the amino acid of SEQ ID NO: 161; the HCDR3 comprises the amino acid of SEQ ID NO: 164; the LCDR1 comprises the amino acid of SEQ ID NO: 169; the LCDR2 comprises the amino acid of SEQ ID NO: 172; and the LCDR3 comprises the amino acid of SEQ ID NO: 175; (ix) the HCDR1 comprises the amino acid of SEQ ID NO: 180; the HCDR2 comprises the amino acid of SEQ ID NO: 183; the HCDR3 comprises the amino acid of SEQ ID NO: 186; the LCDR1 comprises the amino acid of SEQ ID NO: 191; the LCDR2 comprises the amino acid of SEQ ID NO: 194; and the LCDR3 comprises the amino acid of SEQ ID NO: 197; or (x) the HCDR1 comprises the amino acid of SEQ ID NO: 202; the HCDR2 comprises the amino acid of SEQ ID NO: 205; the HCDR3 comprises the amino acid of SEQ ID NO: 208; the LCDR1 comprises the amino acid of SEQ ID NO: 213; the LCDR2 comprises the amino acid of SEQ ID NO: 216; and the LCDR3 comprises the amino acid of SEQ ID NO: 219.
  4. 4 . The method of claim 1 , wherein: (i) the HCDR1 comprises the amino acid of SEQ ID NO: 5; the HCDR2 comprises the amino acid of SEQ ID NO: 8; the HCDR3 comprises the amino acid of SEQ ID NO: 11; the LCDR1 comprises the amino acid of SEQ ID NO: 16; the LCDR2 comprises the amino acid of SEQ ID NO: 19; and the LCDR3 comprises the amino acid of SEQ ID NO: 22; (ii) the HCDR1 comprises the amino acid of SEQ ID NO: 27; the HCDR2 comprises the amino acid of SEQ ID NO: 30; the HCDR3 comprises the amino acid of SEQ ID NO: 33; the LCDR1 comprises the amino acid of SEQ ID NO: 38; the LCDR2 comprises the amino acid of SEQ ID NO: 41; and the LCDR3 comprises the amino acid of SEQ ID NO: 44; (iii) the HCDR1 comprises the amino acid of SEQ ID NO: 49; the HCDR2 comprises the amino acid of SEQ ID NO: 52; the HCDR3 comprises the amino acid of SEQ ID NO: 55; the LCDR1 comprises the amino acid of SEQ ID NO: 60; the LCDR2 comprises the amino acid of SEQ ID NO: 63; and the LCDR3 comprises the amino acid of SEQ ID NO: 66; (iv) the HCDR1 comprises the amino acid of SEQ ID NO: 71; the HCDR2 comprises the amino acid of SEQ ID NO: 74; the HCDR3 comprises the amino acid of SEQ ID NO: 77; the LCDR1 comprises the amino acid of SEQ ID NO: 82; the LCDR2 comprises the amino acid of SEQ ID NO: 85; and the LCDR3 comprises the amino acid of SEQ ID NO: 88; (v) the HCDR1 comprises the amino acid of SEQ ID NO: 93; the HCDR2 comprises the amino acid of SEQ ID NO: 96; the HCDR3 comprises the amino acid of SEQ ID NO: 99; the LCDR1 comprises the amino acid of SEQ ID NO: 104; the LCDR2 comprises the amino acid of SEQ ID NO: 107; and the LCDR3 comprises the amino acid of SEQ ID NO: 110; (vi) the HCDR1 comprises the amino acid of SEQ ID NO: 115; the HCDR2 comprises the amino acid of SEQ ID NO: 118; the HCDR3 comprises the amino acid of SEQ ID NO: 121; the LCDR1 comprises the amino acid of SEQ ID NO: 126; the LCDR2 comprises the amino acid of SEQ ID NO: 129; and the LCDR3 comprises the amino acid of SEQ ID NO: 132; (vii) the HCDR1 comprises the amino acid of SEQ ID NO: 137; the HCDR2 comprises the amino acid of SEQ ID NO: 140; the HCDR3 comprises the amino acid of SEQ ID NO: 143; the LCDR1 comprises the amino acid of SEQ ID NO: 148; the LCDR2 comprises the amino acid of SEQ ID NO: 151; and the LCDR3 comprises the amino acid of SEQ ID NO: 154; (viii) the HCDR1 comprises the amino acid of SEQ ID NO: 159; the HCDR2 comprises the amino acid of SEQ ID NO: 162; the HCDR3 comprises the amino acid of SEQ ID NO: 165; the LCDR1 comprises the amino acid of SEQ ID NO: 170; the LCDR2 comprises the amino acid of SEQ ID NO: 173; and the LCDR3 comprises the amino acid of SEQ ID NO: 176; (ix) the HCDR1 comprises the amino acid of SEQ ID NO: 181; the HCDR2 comprises the amino acid of SEQ ID NO: 184; the HCDR3 comprises the amino acid of SEQ ID NO: 187; the LCDR1 comprises the amino acid of SEQ ID NO: 192; the LCDR2 comprises the amino acid of SEQ ID NO: 195; and the LCDR3 comprises the amino acid of SEQ ID NO: 198; or (x) the HCDR1 comprises the amino acid of SEQ ID NO: 203; the HCDR2 comprises the amino acid of SEQ ID NO: 206; the HCDR3 comprises the amino acid of SEQ ID NO: 209; the LCDR1 comprises the amino acid of SEQ ID NO: 214; the LCDR2 comprises the amino acid of SEQ ID NO: 217; and the LCDR3 comprises the amino acid of SEQ ID NO: 220.
  5. 5 . The method of claim 1 , wherein: (i) the VH comprises the amino acid sequence of SEQ ID NO: 1 and the VL comprises the amino acid sequence of SEQ ID NO: 12; (ii) the VH comprises the amino acid sequence of SEQ ID NO: 23 and the VL comprises the amino acid sequence of SEQ ID NO: 34; (iii) the VH comprises the amino acid sequence of SEQ ID NO: 45 and the VL comprises the amino acid sequence of SEQ ID NO: 56; (iv) the VH comprises the amino acid sequence of SEQ ID NO: 67 and the VL comprises the amino acid sequence of SEQ ID NO: 78; (v) the VH comprises the amino acid sequence of SEQ ID NO: 89 and the VL comprises the amino acid sequence of SEQ ID NO: 100; (vi) the VH comprises the amino acid sequence of SEQ ID NO: 111 and the VL comprises the amino acid sequence of SEQ ID NO: 122; (vii) the VH comprises the amino acid sequence of SEQ ID NO: 133 and the VL comprises the amino acid sequence of SEQ ID NO: 144; (viii) the VH comprises the amino acid sequence of SEQ ID NO: 155 and the VL comprises the amino acid sequence of SEQ ID NO: 166; (ix) the VH comprises the amino acid sequence of SEQ ID NO: 177 and the VL comprises the amino acid sequence of SEQ ID NO: 188; or (x) the VH comprises the amino acid sequence of SEQ ID NO: 199 and the VL comprises the amino acid sequence of SEQ ID NO: 210.
  6. 6 . The method of claim 1 , wherein: (i) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 1 and the light chain variable domain is at least 80% identical to SEQ ID NO: 12; (ii) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 23 and the light chain variable domain is at least 80% identical to SEQ ID NO: 34; (iii) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 45 and the light chain variable domain is at least 80% identical to SEQ ID NO: 56; (iv) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 67 and the light chain variable domain is at least 80% identical to SEQ ID NO: 78; (v) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 89 and the light chain variable domain is at least 80% identical to SEQ ID NO: 100; (vi) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 111 and the light chain variable domain is at least 80% identical to SEQ ID NO: 122; (vii) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 133 and the light chain variable domain is at least 80% identical to SEQ ID NO: 144; (viii) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 155 and the light chain variable domain is at least 80% identical to SEQ ID NO: 166; (ix) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 177 and the light chain variable domain is at least 80% identical to SEQ ID NO: 188; or (x) the heavy chain variable domain is at least 80% identical to SEQ ID NO: 199 and the light chain variable domain is at least 80% identical to SEQ ID NO: 210.
  7. 7 . The method of claim 1 , wherein the antigen binding fragment is a single chain fragment (scFv).
  8. 8 . The method of claim 1 , wherein the cells further comprise a second chimeric antigen receptor that comprises a binding motif that specifically binds an antigen selected from the group consisting of 5T4, alpha-fetoprotein, B cell maturation antigen (BCMA), B cell receptor, CA-125, carcinoembryonic antigen, CD19, CD20, CD22, CD23, CD30, CD33, CD40, CD56, CD79, CD78, CD123, CD138, c-Met, CSPG4, IgM, C-type lectin-like molecule 1 (CLL-1), EGFRvIII, epithelial tumor antigen, ERBB2, FLT3, folate binding protein, GD2, GD3, HER1-HER2 in combination, HER2-HER3 in combination, HER2/Neu, HERV-K, HIV-1 envelope glycoprotein gp41, HIV-1 envelope glycoprotein gp120, IL-11Ralpha, kappa chain, lambda chain, melanoma-associated antigen, mesothelin, MUC-1, mutated p53, mutated ras, prostate-specific antigen, ROR1, VEGFR2, EphA3 (EPH receptor A3), BAFFR (B-cell activating factor receptor), and combinations thereof.
  9. 9 . The method of claim 8 , wherein the binding motif is an anti-CD19 binding motif.
  10. 10 . The method of claim 9 , wherein the anti-CD19 binding motif comprises a heavy chain variable domain (VH) comprising complementarity determining regions HCDR1 comprising the amino acid sequence of SEQ ID NO: 224, HCDR2 comprising the amino acid sequence of SEQ ID NO: 227 and HCDR3 comprising the amino acid sequence of SEQ ID NO: 230, and a light chain variable domain (VL) comprising complementarity determining regions LCDR1 comprising the amino acid sequence of SEQ ID NO: 235, LCDR2 comprising the amino acid sequence of SEQ ID NO: 238 and LCDR3 comprising the amino acid sequence of SEQ ID NO: 241.
  11. 11 . The method of claim 10 , wherein the VH of the anti-CD19 binding motif comprises the amino acid sequence of SEQ ID NO: 221 or an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 221, and the VL of the anti-CD19 binding motif comprises the amino acid sequence of SEQ ID NO: 232 or an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 232.
  12. 12 . The method of claim 9 , wherein the anti-CD19 binding motif is a single chain fragment (scFv).
  13. 13 . The method of claim 12 , wherein the second chimeric antigen receptor further comprises a transmembrane domain that is a transmembrane domain of 4-1BB/CD137, an alpha chain of a T cell receptor, a beta chain of a T cell receptor, CD3 epsilon, CD4, CD5, CD8 alpha, CD9, CD16, CD19, CD22, CD28, CD33, CD37, CD45, CD64, CD80, CD86, CD134, CD137, CD154, or a zeta chain of a T cell receptor, or any combination thereof.
  14. 14 . The method of claim 1 , wherein the cells are CAR-T cells.
  15. 15 . The method of claim 14 , wherein the CAR-T cells are allogeneic CAR-T cells.
  16. 16 . The method of claim 14 , wherein the CAR-T cells are autologous CAR-T cells.
  17. 17 . The method of claim 1 , wherein the B cell cancer is acute lymphoblastic leukemia (ALL), non T cell ALL, acute myeloid leukemia, B cell prolymphocytic leukemia, B cell acute lymphoid leukemia (“BALL”), blastic plasmacytoid dendritic cell neoplasm, Burkitt's lymphoma, chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML), chronic myeloid leukemia, chronic or acute leukemia, diffuse large B cell lymphoma (DLBCL), follicular lymphoma (FL), Hodgkin's Disease, malignant lymphoproliferative conditions, MALT lymphoma, Marginal zone lymphoma, monoclonal gammapathy of undetermined significance (MGUS), myelodysplasia and myelodysplastic syndrome, non-Hodgkin's lymphoma (NHL), plasma cell proliferative disorder, (asymptomatic myeloma, smoldering multiple myeloma, indolent myeloma, plasmablastic lymphoma, plasmacytoid dendritic cell neoplasm, plasmacytomas, (plasma cell dyscrasia, solitary myeloma, solitary plasmacytoma, extramedullary plasmacytoma, multiple plasmacytoma, POEMS syndrome (also known as Crow-Fukase syndrome; Takatsuki disease; and PEP syndrome), small cell- or a large cell-follicular lymphoma, splenic marginal zone lymphoma (SMZL), systemic amyloid light chain amyloidosis, T cell acute lymphoid leukemia (“TALL”), T cell lymphoma, Waldenstrom macroglobulinemia, Mantle cell lymphoma (MCL), Transformed follicular lymphoma (TFL), Primary mediastinal B cell lymphoma (PMBCL), Multiple myeloma, Hairy cell lymphoma/leukemia, or a combination thereof.
  18. 18 . The method of claim 1 , wherein the B cell cancer is diffuse large B cell lymphoma (DLBCL).
  19. 19 . The method of claim 1 , wherein the B cell cancer is relapsed or refractory diffuse large B cell lymphoma (DLBCL).

Description

CROSS-REFERENCE TO RELATED APPLICATIONS The present application is a division of U.S. application Ser. No. 16/711,180, filed Dec. 11, 2019, now U.S. Pat. No. 11,793,834, which claims the priority benefit of U.S. Provisional Application No. 62/778,893, filed Dec. 12, 2018, the contents of each of which are hereby incorporated by reference in their entireties. REFERENCE TO AN ELECTRONIC SEQUENCE LISTING The contents of the electronic sequence listing (347485.xml; Size: 466,983 bytes; and Date of Creation: Oct. 11, 2023) is herein incorporated by reference in its entirety. BACKGROUND OF THE DISCLOSURE Human cancers are by their nature comprised of normal cells that have undergone a genetic or epigenetic conversion to become abnormal cancer cells. In doing so, cancer cells begin to express proteins and other antigens that are distinct from those expressed by normal cells. These aberrant tumor antigens can be used by the body's innate immune system to specifically target and kill cancer cells. However, cancer cells employ various mechanisms to prevent immune cells, such as T and B lymphocytes, from successfully targeting cancer cells. Current therapies T cell therapies rely on enriched or modified human T cells to target and kill cancer cells in a patient. To increase the ability of T cells to target and kill a particular cancer cell, methods have been developed to engineer T cells to express constructs which direct T cells to a particular target cancer cell. Chimeric antigen receptors (CARs) and engineered T cell receptors (TCRs), which comprise binding domains capable of interacting with a particular tumor antigen, allow T cells to target and kill cancer cells that express the particular tumor antigen. A need exists for CARs and TCRs for targeting and killing cancer cells. SUMMARY In at least a first aspect, the present disclosure comprises an antigen binding system, antibody, or antigen binding fragment thereof comprising an anti-CD20 binding motif, wherein the anti-CD20 binding motif comprises sequences of three heavy chain complementarity determining regions (HCDRs) of any one heavy chain variable region (HCVR) selected from the group consisting of SEQ ID NOs: 1, 23, 45, 67, 89, 111, 133, 155, 177, and 199, and sequences of three light chain CDRs (LCDRs) of any one light chain variable region (LCVR) selected from the group consisting of SEQ ID NOs: 12, 34, 56, 78, 100, 122, 144, 166, 188, and 210. In some embodiments, the anti-CD20 binding motif comprises a first domain comprising three heavy chain complementarity determining regions (HCDR1, HCDR2 and HCDR3) and a second domain comprising three light chain complementarity determining regions (LCDR1, LCDR2 and LCDR3), wherein (i) the HCDR1 has a sequence according to any one of SEQ ID NOs: 3-5, 25-27, 47-49, 69-71, 91-93, 113-115, 135-137, 157-159, 179-181; and 201-203; (ii) the HCDR2 has a sequence according to any one of SEQ ID NOs: 6-8, 28-30, 50-52, 72-74, 94-96, 116-118, 138-140, 160-162, 182-184, and 204-206; (iii) the HCDR3 has a sequence according to any one of SEQ ID NOs: 9-11, 31-33, 53-55, 75-77, 97-99, 119-121, 141-143, 163-165, 185-187, and 207-209; (iv) the LCDR1 has a sequence according to any one of SEQ ID NOs: 14-16, 36-38, 58-60, 80-82, 102-104, 124-126, 146-148, 168-170, 190-192, and 212-214; (v) the LCDR2 has a sequence according to any one of SEQ ID NOs: 17-19, 39-41, 61-63, 83-85, 105-107, 127-129, 149-151, 171-173, 193-195; and 215-217; and (vi) the LCDR3 has a sequence according to any one of SEQ ID NOs: 20-22, 42-44, 64-66, 86-88, 108-110, 130-132, 152-154, 174-176, 196-198, and 218-220. In some embodiments, the HCDRs comprise: (i) an HCDR1 according to any of SEQ ID NOs: 3-5; an HCDR2 according to any of SEQ ID NOs: 6-8; an HCDR3 according to any one of SEQ ID NOs: 9-11; (ii) an HCDR1 according to any of SEQ ID NOs: 25-27; an HCDR2 according to any of SEQ ID NOs: 28-30; an HCDR3 according to any one of SEQ ID NOs: 31-33; (iii) an HCDR1 according to any of SEQ ID NOs: 47-49; an HCDR2 according to any of SEQ ID NOs: 50-52; an HCDR3 according to any one of SEQ ID NOs: 53-55; (iv) an HCDR1 according to any of SEQ ID NOs: 69-71; an HCDR2 according to any of SEQ ID NOs: 72-74; an HCDR3 according to any one of SEQ ID NOs: 75-77; (v) an HCDR1 according to any of SEQ ID NOs: 91-93; an HCDR2 according to any of SEQ ID NOs: 94-96; an HCDR3 according to any one of SEQ ID NOs: 97-99; (vi) an HCDR1 according to any of SEQ ID NOs: 113-115; an HCDR2 according to any of SEQ ID NOs: 116-118; an HCDR3 according to any one of SEQ ID NOs: 119-121; (vii) an HCDR1 according to any of SEQ ID NOs: 135-137; an HCDR2 according to any of SEQ ID NOs: 138-140; an HCDR3 according to any one of SEQ ID NOs: 141-143; (viii) an HCDR1 according to any of SEQ ID NOs: 157-159; an HCDR2 according to any of SEQ ID NOs: 160-162; an HCDR3 according to any one of SEQ ID NOs: 163-165; (ix) an HCDR1 according to any of SEQ ID NOs: 179-181; an HCDR2 according to any of SEQ ID N