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US-12624366-B2 - Corn event MON 87411

US12624366B2US 12624366 B2US12624366 B2US 12624366B2US-12624366-B2

Abstract

The invention provides corn event MON 87411, and plants, plant cells, seeds, plant parts, and commodity products comprising event MON 87411. The invention also provides polynucleotides specific for event MON 87411 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87411. The invention also provides methods related to event MON 87411.

Inventors

  • Wen C. Burns
  • Catherine A. Chay
  • Cheryl L. Cloninger
  • Mingqi Deng
  • Stanislaw Flasinski
  • Kunsheng Wu

Assignees

  • MONSANTO TECHNOLOGY LLC

Dates

Publication Date
20260512
Application Date
20220630

Claims (7)

  1. 1 . A transgenic corn plant, wherein the transgenic corn plant comprises a DNA molecule that comprises the nucleotide sequence of SEQ ID NO:4 on chromosome 9 of said transgenic corn plant, and wherein the transgenic corn plant exhibits resistance to corn rootworm infestations and tolerance to herbicide glyphosate relative to a control corn plant that does not comprise the DNA molecule.
  2. 2 . A corn seed that produces the transgenic corn plant of claim 1 .
  3. 3 . The transgenic corn plant of claim 1 , wherein the DNA molecule is derived from event MON 87411.
  4. 4 . The transgenic corn plant of claim 1 , wherein said transgenic corn plant further comprises a transgenic event selected from the group consisting of DAS-59122-7; MON 89034; MON 88017; MIR604; MON 87427; TC1507; 5307; DAS-06275-8; BT176; BT11; and MIR162.
  5. 5 . The transgenic corn plant of claim 1 , wherein the DNA molecule further comprises a nucleotide sequence comprising nucleotides 1-500 of SEQ ID NO: 1 at the 5′ end of the nucleotide sequence of SEQ ID NO:4.
  6. 6 . The transgenic corn plant of claim 1 , wherein the DNA molecule further comprises a nucleotide sequence comprising nucleotides 11749-12248 of SEQ ID NO: 1 at the 3′ end of the nucleotide sequence of SEQ ID NO:4.
  7. 7 . The transgenic corn plant of claim 1 , wherein the DNA molecule further comprises a nucleotide sequence comprising nucleotides 1-500 of SEQ ID NO: 1 at the 5′ end of the nucleotide sequence of SEQ ID NO:4 and nucleotide sequence comprising nucleotides 11749-12248 of SEQ ID NO: 1 at the 3′ end of the nucleotide sequence of SEQ ID NO:4.

Description

REFERENCE TO RELATED APPLICATIONS This application is a continuation of co-pending U.S. application Ser. No. 16/404,513, filed May 6, 2019 (pending), which application is a divisional of U.S. application Ser. No. 15/222,789, filed Jul. 28, 2016 (now U.S. Pat. No. 10,316,330), which application is a divisional of U.S. application Ser. No. 13/890,027, filed May 8, 2013 (now U.S. Pat. No. 9,441,240), which application claims the benefit of U.S. provisional application No. 61/644,368, filed May 8, 2012, the disclosures of which are herein incorporated by reference in their entirety. INCORPORATION OF SEQUENCE LISTING The sequence listing contained in the file named “MONS308US_ST25.txt”, which is 230 kilobytes (size as measured in Microsoft Windows®) and was created on May 6, 2013, is filed herewith by electronic submission and is incorporated by reference herein. FIELD OF THE INVENTION The invention relates to transgenic Zea mays event MON 87411. The event provides dual modes of action for resistance to corn rootworm infestations and tolerance to the herbicide glyphosate. The invention also relates to plants, plant parts, plant seeds, plant cells, agricultural products, and methods related to event MON 87411 and provides nucleotide molecules that are unique to the event and were created in connection with the insertion of transgenic DNA into the genome of a Zea mays plant. BACKGROUND OF THE INVENTION Corn (Zea mays) is an important crop in many areas of the world, and the methods of biotechnology have been applied to this crop in order to produce corn with desirable traits. The expression of an insect resistance or herbicide tolerance transgene in a plant can confer the desirable traits of insect resistance and/or herbicide tolerance on the plant, but expression of such transgenes may be influenced by many different factors including the orientation and composition of the cassettes driving expression of the individual genes transferred to the plant chromosome, and the chromosomal location and the genomic result of the transgene insertion. For example, there can be variation in the level and pattern of transgene expression among individual events that are otherwise identical except for the chromosomal insertion site of the transgene. There may also be undesirable phenotypic or agronomic differences between some events. Therefore, it is often necessary to produce and analyze a large number of individual plant transformation events in order to select an event having superior properties relative to the desirable trait and the optimal phenotypic and agricultural characteristics necessary to make it suitable for commercial purposes. Such selection often requires extensive molecular characterization as well as greenhouse and field trials with many events over multiple years, in multiple locations, and under a variety of conditions so that a significant amount of agronomic, phenotypic, and molecular data may be collected. The resulting data and observations must then be analyzed by teams of scientists and agronomists with the goal of selecting a commercially suitable event. Once selected, such an event may then be used for introgressing the desirable trait into other genetic backgrounds using plant breeding methods, and thus producing a number of different crop varieties that contain the desirable trait and are suitably adapted to specific local growing conditions. To make a transgenic plant containing a single transformation event, a portion of a recombinant DNA construct is transferred into the genome of a corn cell, and the corn cell is subsequently grown into a plant. A corn cell into which the event is initially transferred is regenerated to produce the R0 generation. The R0 plant and progeny plants from the R0 plant can be tested for any desired trait(s), but the effectiveness of the event can be impacted by cis and/or trans factors relative to the integration site in the transformation event. The phenotype conferred by the event can also be impacted by the size and design of the DNA construct, which can vary by the combination of genetic elements in an expression cassette, number of transgenes, number of expression cassettes, and configuration of such elements and such cassettes. Identifying an event with desirable traits can be further complicated by factors such as plant developmental, diurnal, temporal, or spatial patterns of transgene expression; or by extrinsic factors, e.g., environmental plant growth conditions, water availability, nitrogen availability, heat, or stress. Thus, the ability to obtain an event conferring a desirable set of phenotypic traits is not readily predictable. SUMMARY OF THE INVENTION The inventors have identified a transgenic corn event MON 87411 exhibiting superior properties and performance compared to existing transgenic corn plants and to new events constructed in parallel. The corn event MON 87411 contains three linked expression cassettes which collectively confer the traits of