US-12624380-B2 - Micro-object extraction method using diffusiophoresis, and micro-object identification method using same

Abstract

The present invention relates to a micro-object extraction method using diffusiophoresis enabling collection and extraction of micro-objects by using the concentration difference of a solution including the micro-objects to be extracted, and a micro-object identification method using same, wherein the present invention has the following advantages: desired micro-objects can be easily extracted only with a simple device by using diffusiophoresis; the collection and extraction of micro-objects can be easily controlled by changing the type of solution injected into a micro-channel; and energy usage is efficient by using self-powered energy by diffusiophoresis without separate external power required for extracting micro-objects.

Inventors

• Taesung Kim
• Dogyeong Ha

Assignees

• UNIST(ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY)

Dates

Publication Date

20260512

Application Date

20201105

Priority Date

20191119

Claims (9)

1. 1 . A micro-object extraction method using diffusiophoresis, the micro-object extraction method comprising: preparing a micro-object collection and extraction apparatus including a first microchannel and a second microchannel being spaced apart from each other, a collection channel extending from the second microchannel to the first microchannel, and a connection nanochannel connecting the first microchannel to the collection channel; collecting micro-objects into the collection channel by chemiphoresis caused by a concentration difference between a first solution and a second solution and by electrophoresis caused by a diffusivity difference of the first solution by flowing the first solution having a first concentration into the first microchannel and flowing the second solution having a lower second concentration than the first concentration and the micro-objects together into the second microchannel; and extracting the micro-objects from the collection channel to the second microchannel by chemiphoresis caused by a concentration difference between the third solution and the fourth solution and by electrophoresis caused by a diffusivity difference of the third solution by flowing a third solution having a third concentration into the first microchannel and flowing a fourth solution having a lower fourth concentration than the third concentration into the second microchannel.
2. 2 . The micro-object extraction method of claim 1 , wherein, in the collecting of the micro-objects, the micro-objects comprise negatively (−) charged micro-organisms or particles, and the diffusivity difference of the first solution has a negative (−) value.
3. 3 . The micro-object extraction method of claim 1 , wherein, in the extracting of the micro-objects, the micro-objects comprise negatively (−) charged micro-organisms or particles, and the diffusivity difference of the third solution has a positive (+) value.
4. 4 . The micro-object extraction method of claim 1 , wherein, in the collecting of the micro-objects, the micro-objects comprise positively (+) charged micro-organisms or particles, and the diffusivity difference of the first solution has a positive (+) value.
5. 5 . The micro-object extraction method of claim 1 , wherein, in the extracting of the micro-objects, the micro-objects comprise positively (+) charged micro-organisms or particles, and the diffusivity difference of the third solution has a negative (−) value.
6. 6 . The micro-object extraction method of claim 1 , wherein the first solution and the second solution are of same type of solutions having different concentrations.
7. 7 . The micro-object extraction method of claim 6 , wherein the first solution and the second solution are sodium chloride (NaCl) aqueous solutions.
8. 8 . The micro-object extraction method of claim 1 , wherein the third solution and the fourth solution are of same type of solutions having different concentrations.
9. 9 . The micro-object extraction method of claim 8 , wherein the third solution and the fourth solution are potassium acetate (K-acetate) aqueous solutions.

Description

TECHNICAL FIELD The present invention relates to a micro-object extraction method using diffusiophoresis and a micro-object identification method using the method, and more particularly, to a micro-object extraction method using diffusiophoresis enabling collection and extraction of micro-objects by using the concentration difference of a solution including the micro-objects to be extracted, and a micro-object identification method using the method. BACKGROUND ART Point-of-care testing means testing that can be performed in real time without pretreatment of a clinical specimen not in a medical institution but at a site and can be used in diagnosis and treatment, and can be applied to various fields such as disease prevention, disease clinical diagnosis, treatment effect decision, and the like. In point-of-care testing, a perishable material to be tested, such as blood, is immediately diagnosed at a site and thus, there is an advantage of preventing the risk of deterioration or contamination or the like. In particular, such point-of-care testing is essential in Africa or underdeveloped nations of Asia that is difficult to get help from medical institutions. In order for point-of-care testing to be effective, a technology for concentrating a test target material, a technology for separating the test target material from foreign substances, and a technology for extracting the test target material are required. In addition, simplification of an electrical device or a mechanical device is required for a device for point-of-care testing. However, existing technologies take a long time to concentrate the test target material, and equipment therefor is also complicated. DETAILED DESCRIPTION OF THE INVENTION Technical Problem The present invention provides a micro-object extraction method using diffusiophoresis enabling collection and extraction of micro-objects by using the concentration difference of a solution including the micro-objects to be extracted, and a micro-object identification method using the method. Technical solution According to an aspect of the present invention, there is provided a micro-object extraction method using diffusiophoresis, the micro-object extraction method including preparing a micro-object collection and extraction apparatus including a first microchannel and a second microchannel being spaced apart from each other, a collection channel extending from the second microchannel to the first microchannel, and a connection nanochannel connecting the first microchannel to the collection channel, collecting micro-objects into the collection channel by chemiphoresis caused by a concentration difference between the first solution and the second solution and by electrophoresis caused by a diffusivity difference of the first solution by flowing a first solution having a first concentration into the first microchannel and flowing a second solution having a lower second concentration than the first concentration and the micro-objects together into the second microchannel, and extracting the micro-objects from the collection channel to the second microchannel by chemiphoresis caused by a concentration difference between the third solution and the fourth solution and by electrophoresis caused by a diffusivity difference of the third solution by flowing a third solution having a third concentration into the first microchannel and flowing a fourth solution having a lower fourth concentration than the third concentration into the second microchannel. According to another aspect of the present invention, there is provided a micro-object collection and extraction identification method using diffusiophoresis, the micro-object collection and extraction identification method including preparing a micro-object collection and extraction apparatus including a first microchannel and a second microchannel being spaced apart from each other, a collection channel extending from the second microchannel to the first microchannel, and a connection nanochannel connecting the first microchannel to the collection channel, collecting micro-objects into the collection channel by chemiphoresis caused by a concentration difference between the first solution and the second solution and by electrophoresis caused by a diffusivity difference of the first solution by flowing a first solution having a first concentration into the first microchannel and flowing a second solution having a lower second concentration than the first concentration and genetically-modified micro-organisms together into the second microchannel, reacting the micro-organisms collected in the collection channel with a chemical material by flowing the chemical material causing a chemical reaction with the genetically-modified micro-organisms into the first microchannel, and extracting the micro-objects from the collection channel to the second microchannel by chemiphoresis caused by a concentration difference between the third solution and the fourth solu