US-12625106-B2 - Detection base board and detection chip

US12625106B2US 12625106 B2US12625106 B2US 12625106B2US-12625106-B2

Abstract

A detection base board and a detection chip, which relates to the technical field of biological detection. The detection base board includes a plurality of detecting units, each of the detecting units includes a sensing unit and a signal generating unit, and the sensing unit and the signal generating unit are electrically connected; the sensing unit is configured to react with a sample to be detected and generate an electric signal, and the signal generating unit is configured to receive the electric signal and generate a detection current; the sensing unit includes a pair of sensing electrodes, and the pair of sensing electrodes are electrically connected to the signal generating unit; and both of the outer contours of the sensing electrodes include an arc line. The detection base board is used for the fabrication of high-sensitivity and high-accuracy detection chips.

Inventors

ZIJIAN ZHAO
Shinying LAU
Yaqi Zhang

Assignees

Beijing Boe Technology Development Co., Ltd.
BOE TECHNOLOGY GROUP CO., LTD.

Dates

Publication Date: 20260512
Application Date: 20230831

Claims (16)

1 . A detection base board, wherein the detection base board comprises a substrate; the substrate comprises a sample testing region; the sample testing region comprises a plurality of detecting units, each of the detecting units comprises a sensing unit and a signal generating unit, and the sensing unit and the signal generating unit are electrically connected; the sensing unit is configured to react with a sample to be detected and generate an electric signal, and the signal generating unit is configured to receive the electric signal and generate a detection current; the sensing unit comprises a pair of sensing electrodes, and the pair of sensing electrodes are electrically connected to the signal generating unit; and contours of the sensing electrodes comprise an arc line; wherein the signal generating unit comprises a bigrid transistor, and a first grid and a second grid of the bigrid transistor are configured to be connected to different electrically conducting components.
2 . The detection base board according to claim 1 , wherein each of the sensing electrodes comprises a plurality of extending parts and a connecting part connecting the extending parts; in a same sensing electrode, the connecting part has a direction of extension intersecting with a direction of extension of the plurality of extending parts and connects all of the plurality of extending parts together, and the connecting part is located on a same side of the plurality of extending parts; and the sensing unit comprises a first sensing electrode and a second sensing electrode, a pattern of an orthographic projection on the substrate of the connecting part of the first sensing electrode and two extending parts connected to two ends of the connecting part is a first arch, a pattern of an orthographic projection on the substrate of the connecting part of the second sensing electrode and two extending parts connected to two ends of the connecting part is a second arch, and two ends of the first arch extend into a region enclosed by the second arch.
3 . The detection base board according to claim 2 , wherein a quantity of the extending parts of the first sensing electrode is at least two, a quantity of the extending parts of the second sensing electrode is at least three, and the extending parts of the first sensing electrode and the extending parts of the second sensing electrode are alternately arranged; and a shape of a pattern of an orthographic projection of each of the connecting parts on the substrate comprises an arc shape.
4 . The detection base board according to claim 3 , wherein a shape of a pattern of an orthographic projection of the first sensing electrode on the substrate is a U shape; and a shape of a pattern of an orthographic projection on the substrate of the connecting part of the second sensing electrode is an arc shape, and a shape of a pattern of an orthographic projection on the substrate of each of the extending parts of the second sensing electrode is a rectangle.
5 . The detection base board according to claim 1 , wherein the first grid of the bigrid transistor is electrically connected to a constant-voltage-signal inputting terminal, and the first grid is configured to control turning-on and turning-off of the bigrid transistor; and the second grid of the bigrid transistor is electrically connected to the pair of sensing electrodes, and the second grid is configured to control variation of an electric current in the bigrid transistor and simultaneously generating the detection current according to electric signals generated in the sensing electrodes.
6 . The detection base board according to claim 5 , wherein the substrate further comprises a signal-detection region located on at least one side of the sample-detection region, and the signal-detection region comprises at least one negative electrode terminal; and the negative electrode terminal is electrically connected to the second grid by the sensing electrodes.
7 . The detection base board according to claim 6 , wherein the negative electrode terminal is electrically connected to a correcting line, and the negative electrode terminal is configured to release electric charges inside the bigrid transistor to correct the bigrid transistor.
8 . The detection base board according to claim 6 , wherein the signal-detection region comprises a plurality of first signal acquiring terminals, the first signal acquiring terminals are electrically connected to the signal generating units, and a quantity of the first signal acquiring terminals is equal to a quantity of the signal generating units; the signal-detection region further comprises a plurality of second signal acquiring terminals; and in each of the detecting units, a drain of the bigrid transistor is electrically connected to the first signal acquiring terminal, a source of the bigrid transistor is electrically connected to the second signal acquiring terminal; the first signal acquiring terminal, the drain of the bigrid transistor, the source of the bigrid transistor and the second signal acquiring terminal are configured to form an electrically conducting loop, and the detection current refers to an electric current in the loop within a detection time period.
9 . The detection base board according to claim 6 , wherein the sample testing region comprises at least one reference unit, and the reference unit comprises a reference electrode and the bigrid transistor; and in the reference unit, the first grid of the bigrid transistor is electrically connected to the constant-voltage-signal inputting terminal, the first grid is configured to control the turning-on and turning-off of the bigrid transistor, the second grid of the bigrid transistor is electrically connected to the reference electrode, a drain of the bigrid transistor is electrically connected to the first signal acquiring terminal, and a source of the bigrid transistor is electrically connected to the second signal acquiring terminal.
10 . The detection base board according to claim 9 , wherein the sample testing region comprises a first sub-region and a second sub-region; the substrate comprises a first signal-detection sub-region and a second signal-detection sub-region that are located on two sides of the sample testing region; each of the first sub-region and the second sub-region comprises eight detecting units; within the first sub-region, four detecting units are arranged in a first direction, and the other four detecting units are arranged in a second direction, wherein the first direction and the second direction intersect; the first direction and a direction from the first sub-region pointing to the second sub-region form an acute angle therebetween, and the second direction and the direction from the first sub-region pointing to the second sub-region form an acute angle therebetween; and arrangement of the detecting units within the second sub-region and arrangement of the detecting units within the first sub-region are symmetrical.
11 . The detection base board according to claim 10 , wherein the first sub-region comprises one first trace, the second sub-region comprises one second trace, and the first trace and the second trace have equal lengths and are arranged symmetrically; both of the first trace and the second trace extend in the direction from the first sub-region pointing to the second sub-region; and the first trace is configured to connect the eight detecting units within the first sub-region together in series, and the second trace is configured to connect the eight detecting units within the second sub-region together in series.
12 . The detection base board according to claim 11 , wherein the first signal-detection sub-region is located on one side of the first sub-region away from the second sub-region, and the second signal-detection sub-region is located on one side of the second sub-region away from the first sub-region; and the first signal-detection sub-region comprises a first negative electrode terminal, the second signal-detection sub-region comprises a second negative electrode terminal, all of the second grids of all of the bigrid transistors within the first sub-region are electrically connected to the first negative electrode terminal by sequentially the sensing electrodes and the first trace, and all of the second grids of all of the bigrid transistors within the second sub-region are electrically connected to the second negative electrode terminal by sequentially the sensing electrodes and the second trace.
13 . The detection base board according to claim 12 , wherein the detection base board further comprises a plurality of third traces, and a direction of extension of the third traces intersects with the first trace; some of the third traces are configured to connect the first trace and the detecting units within the first sub-region, and the other of the third traces are configured to connect the second trace and the detecting units within the second sub-region; and both of a range of lengths of traces connecting the detecting units and the first negative electrode terminal within the first sub-region and a range of lengths of traces connecting the detecting units and the second negative electrode terminal within the second sub-region are 0.5 cm-5 cm.
14 . The detection base board according to claim 11 , wherein the detection base board comprises a first electrically conducting layer, a first insulating layer, a semiconductor layer, a second insulating layer, a second electrically conducting layer, a third insulating layer, a source-drain electrically conducting layer, a fourth insulating layer and a third electrically conducting layer that are located on the substrate and are sequentially arranged; the first electrically conducting layer comprises the first grids of the bigrid transistors, the semiconductor layer comprises active parts of the bigrid transistors, the second electrically conducting layer comprises the second grids of the bigrid transistors and a plurality of first connecting electrodes, and the source-drain electrically conducting layer comprises the sources and the drains of the bigrid transistors and a plurality of second connecting electrodes; and the sensing electrodes are electrically connected to the second grids of the bigrid transistors by sequentially the second connecting electrodes and the first connecting electrodes.
15 . The detection base board according to claim 10 , wherein within the first sub-region or the second sub-region, in a direction parallel to a plane where the substrate is located, a minimum distance between any two neighboring sensing units is greater than or equal to 1 . 2 cm.
16 . A detection chip, wherein the detection chip comprises the detection base board according to claim 1 , and further comprises a cover plate and an adhesive layer, the cover plate covers the sample testing region of the detection base board, the cover plate is provided with a sample inputting hole and a sample outputting hole, the adhesive layer is located between the detection base board and the cover plate, and the detection base board, the cover plate and the adhesive layer form a cavity therebetween; and all of the detecting units of the detection base board are located inside a same one instance of the cavity.

Description

TECHNICAL FIELD The present application relates to the technical field of biological detection, and particularly relates to a detection base board and a detection chip. BACKGROUND Acute myelogenous leukemia (AML) is a commonly seen adult acute leukemia, and its morbidity increases year by year (1.62/10 ten thousands). AML has a high heterogeneity, and currently there has not been an effective method of early detection. Complete blood cell count and peripheral blood smear are the most commonly seen AML lookup methods. In the blood of most of the AML patients there are many immature leukocytes, while the erythrocytes or the thrombocytes are insufficient. However, those discoveries merely might serve as prompting, and cannot be used as the basis for definite diagnosis, and the definite diagnosis requires the combination with bone marrow biopsy, which causes pain of the patient. Therefore, a non-invasive, quick, high-sensitivity detecting method is currently a research hotspot in AML early diagnosis and prognosis. SUMMARY The embodiments of the present application employ the following technical solutions: In the first aspect, an embodiment of the present application provides a detection base board, wherein the detection base board comprises a substrate; the substrate comprises a sample testing region;the sample testing region comprises a plurality of detecting units, each of the detecting units comprises a sensing unit and a signal generating unit, and the sensing unit and the signal generating unit are electrically connected;the sensing unit is configured to react with a sample to be detected and generate an electric signal, and the signal generating unit is configured to receive the electric signal and generate a detection current;the sensing unit comprises a pair of sensing electrodes, and the pair of sensing electrodes are electrically connected to the signal generating unit; andcontours of the sensing electrodes comprise an are line. In at least one embodiment of the present application, the signal generating unit comprises a bigrid transistor, and a first grid and a second grid of the bigrid transistor are configured to be connected to different electrically conducting components. In at least one embodiment of the present application, the sensing unit further comprises an antibody located on the pair of sensing electrodes, and the antibody and the sensing electrodes are configured to be connected by covalent bonds; types of the antibodies on the pairs of sensing electrodes are different; andthe sensing unit further comprises a surface-treatment layer, the surface-treatment layer covers the pairs of sensing electrodes, and the surface-treatment layer is configured to form the covalent bonds with the antibodies. In at least one embodiment of the present application, each of the sensing electrodes comprises a plurality of extending parts and a connecting part connecting the extending parts; in a same sensing electrode, and the connecting part is located on a same side of the extending parts; andthe sensing unit comprises a first sensing electrode and a second sensing electrode, a pattern of an orthographic projection on the substrate of the connecting part of the first sensing electrode and two extending parts connected to two ends of the connecting part is a first arch, a pattern of an orthographic projection on the substrate of the connecting part of the second sensing electrode and two extending parts connected to two ends of the connecting part is a second arch, and two ends of the first arch extend into a region enclosed by the second arch. In at least one embodiment of the present application, a quantity of the extending parts of the first sensing electrode is at least two, a quantity of the extending parts of the second sensing electrode is at least three, and the extending parts of the first sensing electrode and the extending parts of the second sensing electrode are alternately arranged; and a shape of a pattern of an orthographic projection of each of the connecting parts on the substrate comprises an arc shape. In at least one embodiment of the present application, a shape of a pattern of an orthographic projection of the first sensing electrode on the substrate is a U shape; and a shape of a pattern of an orthographic projection on the substrate of the connecting part of the second sensing electrode is an arc shape, and a shape of a pattern of an orthographic projection on the substrate of each of the extending parts of the second sensing electrode is a rectangle. In at least one embodiment of the present application, the first grid of the bigrid transistor is electrically connected to a constant-voltage-signal inputting terminal, and the first grid is configured to control turning-on and turning-off of the bigrid transistor; and the second grid of the bigrid transistor is electrically connected to the pair of sensing electrodes, and the second grid is configured to control variation of an elect