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US-12625138-B2 - Antibody for porcine reproductive and respiratory syndrome virus and uses thereof

US12625138B2US 12625138 B2US12625138 B2US 12625138B2US-12625138-B2

Abstract

The instant disclosure discloses an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and uses of such antibody or antigen-binding fragment thereof to create immunoassay methods or devices for PRRSV detection.

Inventors

  • Chang-Fu Kuo
  • MING-TANG CHIOU
  • Wei-Hao Lin
  • Lian-Chin WANG
  • AO-HO HSIEH

Assignees

  • NOVASCOPE BIOCHIPS INC.

Dates

Publication Date
20260512
Application Date
20230316

Claims (20)

  1. 1 . An antibody or antigen-binding fragment thereof for binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the antibody or antigen-binding fragment thereof comprising: a heavy chain variable domain comprising: a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO: 1, a CDR-H2 comprising the sequence of SEQ ID NO: 2, and a CDR-H3 comprising the sequence of SEQ ID NO: 3; and a light chain variable domain comprising: a light chain complementarity-determining region 1 (CDR-L1) comprising the sequence of SEQ ID NO: 4, a CDR-L2 comprising the sequence of Ala-Ala-Ser, and a CDR-L3 comprising the sequence of SEQ ID NO: 5.
  2. 2 . The antibody or antigen-binding fragment thereof of claim 1 , wherein the heavy chain variable domain further comprises the sequence of SEQ ID NO: 6.
  3. 3 . The antibody or antigen-binding fragment thereof of claim 1 , wherein the light chain variable domain further comprises the sequence of SEQ ID NO: 7.
  4. 4 . An isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of claim 1 , wherein the isolated nucleic acid encoding the antibody or antigen-binding fragment thereof comprises a first fragment encoding the heavy chain variable domain and a second fragment encoding the light chain variable domain.
  5. 5 . The isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of claim 4 , wherein the first fragment encoding the heavy chain variable domain comprises the sequence of SEQ ID NO: 8.
  6. 6 . The isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of claim 4 , wherein the second fragment encoding the light chain variable domain comprises the sequence of SEQ ID NO: 9.
  7. 7 . A vector comprising the isolated nucleic acid of claim 4 .
  8. 8 . A host cell comprising the vector of claim 7 .
  9. 9 . A method for producing an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the method comprising: (a) culturing the host cell of claim 8 under conditions suitable for expressing the antibody or antigen-binding fragment thereof; and (b) recovering the antibody or antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof comprises a heavy chain variable domain and a light chain variable domain.
  10. 10 . The method for producing an antibody or antigen-binding fragment thereof binding to PRRSV of claim 9 , wherein the heavy chain variable domain further comprises the sequence of SEQ ID NO: 6.
  11. 11 . The method for producing an antibody or antigen-binding fragment thereof binding to PRRSV of claim 9 , wherein the light chain variable domain further comprises the sequence of SEQ ID NO: 7.
  12. 12 . A method for detecting Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the method comprising contacting a sample with the antibody or antigen-binding fragment thereof of claim 1 .
  13. 13 . The method of claim 12 , wherein the heavy chain variable domain further comprises the sequence of SEQ ID NO: 6.
  14. 14 . The method of claim 12 , wherein the light chain variable domain further comprises the sequence of SEQ ID NO: 7.
  15. 15 . A biological field-effect transistor (Bio-FET), comprising: a transistor region; and a detecting region, wherein the detecting region comprises a detecting surface that is functionalized with an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), wherein the antibody or antigen-binding fragment thereof comprises: a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO: 1, a CDR-H2 comprising the sequence of SEQ ID NO: 2, and a CDR-H3 comprising the sequence of SEQ ID NO: 3; and a light chain variable domain comprising a light chain complementarity-determining region 1 (CDR-L1) comprising the sequence of SEQ ID NO: 4, a CDR-L2 comprising the sequence of Ala-Ala-Ser, and a CDR-L3 comprising the sequence of SEQ ID NO: 5.
  16. 16 . The Bio-FET of claim 15 , wherein the heavy chain variable domain further comprises the sequence of SEQ ID NO: 6.
  17. 17 . The Bio-FET of claim 15 , wherein the light chain variable domain further comprises the sequence of SEQ ID NO: 7.
  18. 18 . A method for detecting Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by using a biological field-effect transistor (Bio-FET), the method comprising: (a) contacting a sample with an antibody or antigen-binding fragment thereof immobilized on a detecting surface of the Bio-FET, wherein the antibody or antigen-binding fragment thereof comprises: a heavy chain variable domain comprising a heavy chain complementarity-determining region 1 (CDR-H1) comprising the sequence of SEQ ID NO: 1, a CDR-H2 comprising the sequence of SEQ ID NO: 2, and a CDR-H3 comprising the sequence of SEQ ID NO: 3; and light chain variable domain comprising a CDR-L1 comprising the sequence of SEQ ID NO: 4, a CDR-L2 comprising the sequence of Ala-Ala-Ser, and a CDR-L3 comprising the sequence of SEQ ID NO: 5, wherein the antibody or antigen-binding fragment thereof binds to PRRSV; and (b) analyzing an electric signal obtained from the Bio-FET.
  19. 19 . The method of claim 18 , wherein the heavy chain variable domain further comprises the sequence of SEQ ID NO: 6.
  20. 20 . The method of claim 18 , wherein the light chain variable domain further comprises the sequence of SEQ ID NO: 7.

Description

CROSS-REFERENCE TO RELATED APPLICATION This application claims benefit of and priority to U.S. Provisional Patent Application Ser. No. 63/321,209, entitled “ANTIBODY FOR PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV)”, filed on Mar. 18, 2022. The contents of the above-mentioned application are hereby incorporated by reference herein for all purposes. REFERENCE TO SEQUENCE LISTING Accompanying this application is a sequence listing in an XML file named “2022A-149447-SequenceListing.xml”, created Mar. 16, 2023, and having a size of 13,755 bytes. The sequence listing is hereby fully incorporated by reference herein. FIELD The present disclosure generally relates to an antibody or an antigen-binding fragment and, more specifically, relates to an antibody or an antigen-binding fragment for recognizing Porcine Reproductive and Respiratory Syndrome Virus and use of the same. BACKGROUND Porcine reproductive and respiratory syndrome (PRRS) is viewed by many as the most important disease currently affecting the pig industry worldwide. Porcine reproductive and respiratory syndrome causes severe reproduction losses, is associated with increased mortality due to secondary infections, and is linked to reduced feed conversion and average daily weight gain. Unfortunately, control of the virus that causes PRRS has proven to be difficult. PRRSV is an enveloped single-stranded RNA virus classified in the family Arteriviridae (Cavanaugh, 1997). The virus causes a widespread disease of swine that was first described as a ‘mystery swine disease’ in the USA in 1987 (Hill, 1990). The disease manifests as respiratory illness in all age groups of swine leading to death in some younger pigs and severe reproductive problems in breeding-age females. PRRSV infection is widespread on most pig farms in the world. PRRSV has been found to cause low survival rates and low feed conversion rates (FCRs) of infected pigs and results in great economic losses in pig farming. There are some PRRSV test kits for commercial use. However, the PRRSV test kits that are commercially available typically involve long testing times and involve a need to prepare more reagents. Therefore, it is important to develop PRRSV test kits that can detect PRRSV more easily and quickly. SUMMARY The present disclosure is directed to an antibody or an antigen-binding fragment and, more specifically, to an antibody or an antigen-binding fragment for recognizing Porcine Reproductive and Respiratory Syndrome Virus and use of the same. According to a first aspect of the present disclosure, an antibody or antigen-binding fragment thereof binding to PRRSV is provided, which includes: a heavy chain variable domain including a heavy chain complementarity-determining region 1 (CDR-H1) containing a sequence of SEQ ID NO: 1, a CDR-H2 containing a sequence of SEQ ID NO: 2, and a CDR-H3 containing a sequence of SEQ ID NO: 3; and a light chain variable domain including a light chain complementarity-determining region 1 (CDR-L1) containing a sequence of SEQ ID NO: 4, a CDR-L2 containing a sequence of Ala-Ala-Ser, and a CDR-L3 containing a sequence of SEQ ID NO: 5. In an implementation of the first aspect of the present disclosure, the heavy chain variable domain further includes a sequence of SEQ ID NO: 6. In another implementation of the first aspect of the present disclosure, the light chain variable domain further includes a sequence of SEQ ID NO: 7. According to a second aspect of the present disclosure, an isolated nucleic acid encoding the antibody or antigen-binding fragment thereof of the first aspect of the present disclosure is provided, the isolated nucleic acid encoding the antibody or antigen-binding fragment thereof comprises a first fragment encoding a heavy chain variable domain and a second fragment encoding a light chain variable domain. In an implementation of the second aspect of the present disclosure, the first fragment encoding the heavy chain variable domain further includes a sequence of SEQ ID NO: 8. In an implementation of the first aspect of the present disclosure, the second fragment encoding the light chain variable domain further includes a sequence of SEQ ID NO: 9. According to a third aspect of the present disclosure, a vector including the isolated nucleic acid of the second aspect of the present disclosure is provided. According to a fourth aspect of the present disclosure, a host cell including the vector of the third aspect of the present disclosure is provided. According to a fifth aspect of the present disclosure, a method for producing an antibody or antigen-binding fragment thereof binding to PRRSV is provided, which includes: (a) culturing the host cell of the fourth aspect of the present disclosure under conditions suitable for expressing the antibody or antigen-binding fragment thereof; and (b) recovering the antibody or antigen-binding fragment thereof. In an implementation of the fifth aspect of the present disclosure, the heavy