US-20240219413-A1 - Reagent Preparation Apparatus and Nucleic Acid Testing Integrated Machine with Reagent Preparation Apparatus

US20240219413A1US 20240219413 A1US20240219413 A1US 20240219413A1US-20240219413-A1

Abstract

The disclosure provides a reagent preparation apparatus and a nucleic acid testing integrated machine having the reagent preparation apparatus. The reagent preparation apparatus includes: a housing, the housing being provided with an extraction reagent filling position and an amplification reagent filling position; a reagent storage portion, arranged in the housing, the reagent storage portion including an extraction reagent storage portion and an amplification reagent storage portion; and a filling portion, movably arranged in the housing, the filling portion being able to suck an extraction reagent from the extraction reagent storage portion and move to above the extraction reagent filling position to fill the extraction reagent into a first vessel on the extraction reagent filling position, and the filling portion being able to suck an amplification reagent from the amplification reagent storage portion and move to above the amplification reagent filling position to fill the amplification reagent into a second vessel on the amplification reagent filling position.

Inventors

WANG YIXIAN
ZHENG XIAOLIN
HE GUOYAO
LIU ZHENHUA
XIONG YINGCHAO
ZHANG TINGTING
Chen Muxiao
MA JUANJUAN
SHENG XUEQING

Assignees

SHENZHEN NEW IND BIOMEDICAL ENGINEERING CO LTD

Dates

Publication Date: 20240704
Application Date: 20231130
Priority Date: 20221231

Claims (20)

1 . A reagent preparation apparatus, comprising: a housing, the housing being provided with an extraction reagent filling position and an amplification reagent filling position; a reagent storage portion, arranged in the housing, the reagent storage portion comprising an extraction reagent storage portion and an amplification reagent storage portion; and a filling portion, movably arranged in the housing, the filling portion being able to suck an extraction reagent from the extraction reagent storage portion and move to above the extraction reagent filling position to fill the extraction reagent into a first vessel on the extraction reagent filling position, and the filling portion being able to suck an amplification reagent from the amplification reagent storage portion and move to above the amplification reagent filling position to fill the amplification reagent into a second vessel on the amplification reagent filling position.
2 . The reagent preparation apparatus according to claim 1 , wherein the housing is provided with a first platform and a second platform located below the first platform, the first platform and the second platform are parallel to a horizontal plane, the extraction reagent storage portion, the amplification reagent storage portion, the extraction reagent filling position, and the amplification reagent filling position are arranged on the second platform, and the filling portion is arranged on the first platform.
3 . The reagent preparation apparatus according to claim 2 , further comprising a first vessel loading mechanism configured to load the first vessel and a grabbing piece, wherein the first vessel loading mechanism comprises a first vessel loading bin and a first vessel transfer bin; a top of the first vessel transfer bin is provided with a first opening, and the first opening extends to above the second platform; and the grabbing piece is movably arranged on the first platform, and the grabbing piece is able to grab the first vessel through the first opening and move to the extraction reagent filling position.
4 . The reagent preparation apparatus according to claim 3 , the housing is further provided with a third platform located below the second platform and parallel to the horizontal plane; the first vessel loading bin is movably arranged on the third platform and able to move to an outside of the housing; the first vessel transfer bin is fixedly arranged on the third platform.
5 . The reagent preparation apparatus according to claim 3 , wherein the first vessel loading bin and the first vessel transfer bin extend in a Z-axis direction and are arranged at an interval in an X-axis direction, the housing is provided with a first side opening, and the first vessel loading bin is able to move to the outside of the housing through the first side opening.
6 . The reagent preparation apparatus according to claim 2 , further comprising a second vessel loading mechanism configured to load the second vessel and a grabbing piece, wherein the second vessel loading mechanism comprises a second vessel loading bin, the second vessel loading bin is provided with a second opening, and the grabbing piece is movably arranged on the first platform, the gripping piece is able to grab the second vessel through the second opening.
7 . The reagent preparation apparatus according to claim 2 , wherein further comprising a first vessel loading mechanism configured to load the first vessel, a second vessel loading mechanism configured to load the second vessel and a grabbing piece, the grabbing piece is able to grab the first vessel and the second vessel.
8 . The reagent preparation apparatus according to claim 6 , wherein the second vessel loading mechanism further comprises a second vessel storage bin; a top of the second vessel storage bin is provided with a third opening; and the grabbing piece is able to transfer the second vessel from the second vessel loading bin to the second vessel storage bin, and the grabbing piece is able to grab the second vessel through the third opening and move to the amplification reagent filling position.
9 . The reagent preparation apparatus according to claim 2 , wherein further comprising a first vessel loading mechanism configured to load the first vessel and a second vessel loading mechanism configured to load the second vessel, the first vessel loading mechanism comprises a first vessel loading bin, the second vessel loading mechanism comprises a second vessel loading bin; the housing is provided with a first side opening, the first vessel loading bin is able to move to the outside of the housing through the first side opening, the housing is further provided with a second side opening located above the first side opening, the second vessel loading bin is able to move to the outside of the housing through the second side opening.
10 . The reagent preparation apparatus according to claim 8 , wherein the second vessel storage bin is fixedly arranged on the second platform and extends in the Z-axis direction, and the second vessel loading bin is movably arranged on the second platform in the X-axis direction and extends in the Z-axis direction.
11 . The reagent preparation apparatus according to claim 6 , wherein the extraction reagent storage portion comprises a first reagent storage portion and a second reagent storage portion, wherein the first reagent storage portion is arranged on the second platform, the second reagent storage portion is arranged outside of the housing, the first reagent storage portion ( 211 ) and the second vessel loading bin ( 61 ) are arranged at an interval in the X-axis direction, and the first reagent storage portion ( 211 ) is able to move to the outside of the housing ( 10 ) with the second vessel loading bin ( 61 ).
12 . The reagent preparation apparatus according to claim 3 , wherein the second platform is further provided with a premixing position configured to place the first vessel, and the grabbing piece is able to grab the first vessel through the first opening and move to the premixing position.
13 . The reagent preparation apparatus according to claim 2 , the filling portion further comprises a reagent arm assembly, wherein the reagent arm assembly comprises a reagent arm and a pipetting needle, wherein the reagent arm is movably arranged on the first platform in the X-axis direction and the Y-axis direction, the pipetting needle is movably arranged on the reagent arm in the Z-axis direction, the pipetting needle is able to suck the extraction reagent so as to fill the extraction reagent into the first vessel, and the pipetting needle is able to suck the amplification reagent and move to above the amplification reagent filling position to fill the amplification reagent into the second vessel.
14 . The reagent preparation apparatus according to claim 11 , wherein, the filling portion comprises a filling assembly, wherein the filling assembly comprises a moving frame and a liquid injection needle, wherein the moving frame is movably arranged in the housing in a Y-axis direction, the liquid injection needle is movably arranged on the moving frame in the Z-axis direction, and the liquid injection needle communicates with the second reagent storage portion to fill a second reagent into the first vessel; and the filling portion further comprises a reagent arm assembly, wherein the reagent arm assembly comprises a reagent arm and a pipetting needle, wherein the reagent arm is movably arranged on the first platform in the X-axis direction and the Y-axis direction, the pipetting needle is movably arranged on the reagent arm in the Z-axis direction, the pipetting needle is able to move to above the first reagent storage portion to suck a first reagent so as to fill the first reagent into the first vessel, and the pipetting needle is able to move to above the amplification reagent storage portion to suck the amplification reagent and move to above the amplification reagent filling position to fill the amplification reagent into the second vessel.
15 . The reagent preparation apparatus according to claim 13 , wherein the first reagent comprises an elution solution and magnetic beads, the second reagent comprises lysate, a first washing liquid, a second washing liquid, and a third washing liquid.
16 . The reagent preparation apparatus according to claim 14 , wherein the first platform is provided with a first guide rail and a second guide rail, the first guide rail extends in the Y-axis direction, the second guide rail extends in the X-axis direction, the first guide rail is movably arranged below the second guide rail in an extension direction of the second guide rail, and the reagent arm is movably arranged on the first guide rail in an extension direction of the first guide rail; wherein the first platform is further provided with a third guide rail extending in the Y-axis direction, and the third guide rail is movably arranged below the second guide rail in the extension direction of the second guide rail; and the grabbing piece comprises a grabbing frame and a gripper, wherein the grabbing frame is movably arranged on the third guide rail in an extension direction of the third guide rail, and the gripper is movably arranged on the grabbing frame in the Z-axis direction.
17 . The reagent preparation apparatus according to claim 14 , further comprising a consumable storage bin arranged on the second platform and configured to carry a magnetic rod sleeve and a pipette tip, wherein the pipetting needle is able to move to above the consumable storage bin to load the magnetic rod sleeve and the pipette tip, and the consumable storage bin and the first reagent storage portion extend in the X-axis direction and are arranged at an interval in the Y-axis direction.
18 . The reagent preparation apparatus according to claim 2 , further comprising a first vessel transfer assembly arranged on the second platform, wherein the first vessel transfer assembly comprises a transfer frame, a sliding plate, and a sliding plate driving piece, wherein the extraction reagent filling position is arranged on an upper surface of the sliding plate, the transfer frame is arranged on the second platform and extends in the X-axis direction, the sliding plate driving piece is arranged on the transfer frame, and the sliding plate driving piece is in drive connection with the sliding plate, so that the sliding plate moves in an extension direction of the transfer frame.
19 . The reagent preparation apparatus according to claim 1 , wherein an upper surface of the extraction reagent filling position is lower than an upper surface of the amplification reagent filling position.
20 . A nucleic acid testing integrated machine, comprising a reagent preparation apparatus, a to-be-tested object extraction apparatus, and an amplification apparatus, wherein the to-be-tested object extraction apparatus is configured to extract an analyte from a sample and mix the analyte with the amplification reagent to form a determination mixture, the amplification apparatus is configured to perform analyte detection determination on the determination mixture to analyze the sample, and the reagent preparation apparatus is the reagent preparation apparatus according to claim 1 .

Description

CROSS REFERENCE TO RELATED APPLICATIONS The present disclosure claims benefit of Chinese Patent Application No. 202211738460.5, filed on Dec. 31, 2022, entitled “Reagent Preparation Apparatus and Nucleic Acid Testing Integrated Machine with Reagent Preparation Apparatus”, the contents of which are hereby incorporated by reference in its entirety. TECHNICAL FIELD The disclosure relates to a technical field of biological detection, in particular to a reagent preparation apparatus and a nucleic acid testing integrated machine with the reagent preparation apparatus. BACKGROUND Nucleic acid testing usually needs to extract nucleic acid from a subject sample, then perform an amplification reaction, and further analyze a situation of the nucleic acid in a subject body by detecting an amplification result. This testing process involves the addition of a variety of extraction reagents and amplification reagents, as well as the loading and use of a variety of vessel consumables. In the related art, the preparation of the extraction reagents and the amplification reagents is often allocated to a plurality of instrument modules, which easily leads to the problem that a nucleic acid testing instrument is too large. However, the space in hospital laboratories is limited, and a method of arranging a plurality of modules separately for reagent addition is difficult to meet the requirements for miniaturization of the nucleic acid testing instrument on the market. SUMMARY The disclosure provides a reagent preparation apparatus and a nucleic acid testing integrated machine with the reagent preparation apparatus, so as to solve the problem that a nucleic acid testing instrument in the related technology cannot meet the requirements for miniaturization. According to one aspect of the disclosure, a reagent preparation apparatus is provided. The reagent preparation apparatus includes: a housing, the housing being provided with an extraction reagent filling position and an amplification reagent filling position; a reagent storage portion, arranged in the housing, the reagent storage portion including an extraction reagent storage portion and an amplification reagent storage portion; and a filling portion, movably arranged in the housing, the filling portion being able to suck the extraction reagent from the extraction reagent storage portion and move to above the extraction reagent filling position to fill the extraction reagent into a first vessel on the extraction reagent filling position, and the filling portion being able to suck the amplification reagent from the amplification reagent storage portion and move to above the amplification reagent filling position to fill the amplification reagent into a second vessel on the amplification reagent filling position. In some embodiments, the housing is provided with a first platform and a second platform located below the first platform, the first platform and the second platform are parallel to a horizontal plane, the extraction reagent storage portion, the amplification reagent storage portion, the extraction reagent filling position, and the amplification reagent filling position are arranged on the second platform, and the filling portion is arranged on the first platform. In some embodiments, the reagent preparation apparatus further includes a first vessel loading mechanism configured to load the first vessel and a grabbing piece. The first vessel loading mechanism includes a first vessel loading bin and a first vessel transfer bin. The top of the first vessel transfer bin is provided with a first opening, and the first opening extends to above the second platform. The grabbing piece is movably arranged on the first platform, and the grabbing piece is able to grab the first vessel through the first opening and move to the extraction reagent filling position. In some embodiments, the housing is further provided with a third platform located below the second platform and parallel to the horizontal plane. The first vessel loading bin is movably arranged on the third platform and able to move to the outside of the housing. The first vessel transfer bin is fixedly arranged on the third platform. In some embodiments, the first vessel loading bin and the first vessel transfer bin extend in a Z-axis direction and are arranged at an interval in an X-axis direction, the housing is provided with a first side opening, and the first vessel loading bin is able to move to the outside of the housing through the first side opening. In some embodiments, the reagent preparation apparatus further includes a second vessel loading mechanism configured to load the second vessel and a grabbing piece. The second vessel loading mechanism includes a second vessel loading bin. The second vessel loading bin is provided with a second opening, and the grabbing piece is movably arranged on the first platform, the gripping piece is able to grab the second vessel through the second opening. In some embodiments