US-20260123631-A1 - NATURAL PORPHIN SALT AND USE THEREOF AS PLANT GROWTH REGULATOR AND PLANT IMMUNITY INDUCER

US20260123631A1US 20260123631 A1US20260123631 A1US 20260123631A1US-20260123631-A1

Abstract

The present disclosure provides a natural porphin salt and a use thereof as a plant growth regulator and a plant immunity inducer. The porphin salt includes a salt of porphin or a salt of chlorin. Compared with the prior art, the porphin salt of the present disclosure has high stability, exhibits a stable and reliable quality during long-term storage, has a long shelf life, and is easy to carry and transport. The porphin salt has prominent water solubility, is easy to prepare into an aqueous solution with a stable content, and exhibits excellent reproducibility when applied to the field. The porphin salt can be conveniently applied at a low concentration with a strong activity and a prominent control effect. Raw materials for preparing the porphin salt are natural and widely available. The preparation of the porphin salt is emission-free, pollution-free, energy-saving, and eco-friendly.

Inventors

Yong Ren
Lihui WEI
Letian Wang
Dongmei Zhou
Dongfeng MENG

Assignees

NANJING BOSTEC BIOLOGICAL ENGINEERING CO., LTD

Dates

Publication Date: 20260507
Application Date: 20220906

Claims (19)

1 . A use of a porphin salt as a plant growth regulator or a plant immunity inducer, wherein the porphin salt comprises a salt of a porphin compound or a salt of a chlorin compound.
2 . The use according to claim 1 , wherein the salt of the porphin compound comprises a salt of protoporphyrin or a salt of a protoporphyrin chelate; and the salt of the chlorin compound comprises a salt of pheophorbide or a salt of a pheophorbide chelate.
3 . The use according to claim 1 , wherein the porphin compound is selected from a group consisting of protoporphyrin, chlorhematin, and hydroxyhematin; and the chlorin compound is selected from a group consisting of pheophorbide, iron chlorin (iron chlorophyllin chloride), iron hydroxychlorophyllin, iron chlorophyllin, zinc chlorophyllin, and copper chlorophyllin.
4 . The use according to claim 1 , wherein the salt of the porphin compound is prepared through a salt-producing reaction of acidic protoporphyrin or a chelate thereof with a metal ion; and the salt of the chlorin compound is prepared through a salt-producing reaction of acidic pheophorbide or a chelate thereof with a metal ion.
5 . The use according to claim 1 , wherein the salt of the porphin compound comprises a monovalent, divalent, or trivalent metal ion salt of the porphin compound; and the salt of the chlorin compound comprises a monovalent, divalent, or trivalent metal ion salt of the chlorin compound.
6 . The use according to claim 1 , wherein the salt of the porphin compound comprises a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, or copper salt of the porphin compound; and the salt of the chlorin compound comprises a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, or copper salt of the chlorin compound.
7 . The use according to claim 1 , wherein the porphin salt is one of or a combination of two or more of: a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of protoporphyrin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of chlorhematin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of hydroxyhematin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron chlorin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron hydroxychlorophyllin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron chlorophyllin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of chlorin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of zinc chlorophyllin, and a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of copper chlorophyllin.
8 . The use according to claim 1 , wherein a preparation method of the porphin salt comprises: allowing an acidic porphin compound or an acidic chlorin compound to react with a monovalent metal hydroxide to produce a precipitate, and separating the precipitate to obtain a monovalent metal ion salt of the porphin compound or the chlorin compound; or allowing a monovalent metal salt of the porphin compound or the chlorin compound to react with a soluble polyvalent metal ion salt to produce a precipitate, and separating the precipitate to obtain a polyvalent metal ion salt of the porphin compound or the chlorin compound.
9 . The use according to claim 8 , wherein the monovalent metal hydroxide is selected from a group consisting of sodium hydroxide, potassium hydroxide, and ammonia water; and in a preparation process of the polyvalent metal ion salt of the porphin compound or the chlorin compound, the monovalent metal salt of the porphin compound or the chlorin compound is selected from a group consisting of sodium and potassium salts of the porphin compound or the chlorin compound; and the soluble polyvalent metal ion salt comprises a sulfate, a hydrochloride, or a nitrate of a divalent or trivalent metal ion.
10 . The use according to claim 8 , wherein a preparation process of the monovalent metal ion salt of the porphin compound or the chlorin compound comprises following steps: preparing the acidic porphin compound or the acidic chlorin compound into a solution with an organic solvent, and mixing with an organic solvent of a monovalent metal ion salt for precipitation; or subjecting the acidic porphin compound or the acidic chlorin compound and the monovalent metal ion salt to a salt-producing reaction in an aqueous solution, and conducting precipitation with an organic solvent; or preparing the acidic porphin compound or the acidic chlorin compound into a solution with an organic solvent, and introducing a dry ammonia gas into the solution to produce a precipitate; and finally conducting filtration, washing, and drying; and a preparation process of the polyvalent metal ion salt of the porphin compound or the chlorin compound comprises following steps: mixing an aqueous solution of the monovalent metal salt of the porphin compound or the chlorin compound with an aqueous solution of the soluble polyvalent metal ion salt, and conducting precipitation, filtration, washing, and drying.
11 . (canceled)
12 . (canceled)
13 . The use according to claim 1 , wherein comprising: applying the porphin salt through spraying, smearing, seed soaking, aerial application, ear soaking, irrigation, or sprinkling to a plant requiring growth regulation or stress resistance enhancement or an environment in which the plant grows to regulate growth of the plant or enhance stress resistance of the plant.
14 . The use according to claim 1 , wherein the porphin salt is applied at a concentration/content of 0.001 ppm to 10 ppm.
15 . The use according to claim 1 , wherein in case of seed soaking and field irrigation, the porphin salt is applied at a concentration/content of 0.001 ppm to 0.1 ppm; and in case of foliar spraying, the porphin salt is applied at a concentration/content of 0.01 ppm to 10 ppm.
16 . The use according to claim 1 , wherein effects of the plant growth regulator comprise promoting seed germination, improving a germination rate, increasing a root length, promoting growth of a root, enhancing immunity and stress resistance of a plant, accelerating growth of a seedling, increasing a chlorophyll content, delaying premature senescence of a plant, and improving a yield and a quality.
17 . The use according to claim 1 , wherein effects of the plant immunity inducer comprise improving stress resistance of a plant and increasing a yield of a crop.
18 . A use of a composition comprising a porphin salt as a plant growth regulator or a plant immunity inducer, wherein the porphin salt comprises a salt of a porphin compound or a salt of a chlorin compound.
19 . The use according to claim 18 , wherein the porphin salt is one of or a combination of two or more of: a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of protoporphyrin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of chlorhematin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of hydroxyhematin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron chlorin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron hydroxychlorophyllin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of iron chlorophyllin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of chlorin, a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of zinc chlorophyllin, and a sodium, potassium, ammonium, magnesium, calcium, ferrous iron, ferric iron, zinc, manganese, and copper salt of copper chlorophyllin.

Description

REFERENCE TO A SEQUENCE LISTING The instant application contains a Sequencing Listing which has been submitted electronically in XML file and is hereby incorporated by reference in its entirety. Said XML copy, created on Nov. 7, 2025, is named 153409-sequencing listing and is 13,893 bytes in size. TECHNICAL FIELD The present disclosure relates to a natural porphin salt and a use thereof as a plant growth regulator and a plant immunity inducer, and belongs to the technical field of plant growth regulators. BACKGROUND Chlorophyll and heme are natural porphin structures produced and existing in animals and plants. Chlorophyll and heme are a material basis for life-sustaining activities such as photosynthesis and aerobic respiration in animals and plants. Heme has a classical porphin structure. Chlorophyll has a chlorin parent nucleus structure. Chlorin has more significant asymmetry and exists in more complex and diverse forms and states than porphin. Various porphin products produced through natural extraction and processing have begun to be widely used in many fields such as chemicals, medicine, food, and agriculture. For example, iron chlorin (iron chlorophyllin chloride, CN102285992B) and chlorhematin (heme, CN10048884C) have been used as novel plant growth regulators in a variety of crops. Sodium iron chlorophyllin and potassium/sodium copper chlorophyllin are used in food additives (food colorants). Protoporphyrin disodium is used for treating liver cirrhosis. In addition to commercially-available products, the Chinese patents CN101045730, CN102351867, CN102775416, CN102796108, etc. have disclosed the preparation and properties of bivalent salts such as zinc iron chlorophyllin, calcium iron chlorophyllin, magnesium iron chlorophyllin, and manganese iron chlorophyllin in recent years. In the literature (Study on Reaction Rates, Equilibrium Constants, and Structures of Metallic Salts of Iron Chlorophyllin, Master's Thesis, North China University of Science and Technology, 2017.03), the preparation of a series of metallic salts of iron chlorophyllin is investigated, including salt-producing reactions, product structures, and physical and chemical properties. Study results have shown that iron chlorophyllin can be combined with divalent ions Mg2+, Ca2+, Mn2+, and Zn2+ in a molar ratio of 1:1 to produce slightly-soluble/insoluble salts (Ksp≈10−16 to 10−18 M2), and salt-producing reactions are fast (the activation energy is about 20 kJ/mol). Taking advantage of the difference in water solubility, the divalent metallic salts of these porphin chelates can be prepared through the precipitation from aqueous solutions, and the water-soluble porphin sodium is the basic raw material for preparing these products. The reported water-soluble porphin salts are mainly sodium/potassium salts of divalent transition metal-porphin chelates, such as sodium zinc chlorophyllin, potassium zinc chlorophyllin, sodium manganese chlorophyllin, and sodium copper chlorophyllin. These sodium/potassium salts have excellent solubility, and the conventional aqueous solutions of these sodium/potassium salts are strongly alkaline. However, there are few reports on sodium/potassium salts of trivalent transition metal-porphin chelates. Chlorhematin is a trivalent iron-porphin chelate, where chlorine axially binding to central iron is easily substituted with hydroxyl having a stronger coordination capacity than chlorine. Thus, sodium hydroxyhematin can be produced when an alkali and chlorhematin are added to an aqueous solution (Properties and detection methods of chlorhematin, Chinese Journal of Biochemical Pharmaceutics, 1993.66 (4): 58-59). Experiments have proved that, through the precipitation of an alkaline aqueous solution, only sodium hydroxyhematin can be prepared, but sodium chlorhematin cannot be produced. The use of sodium iron chlorophyllin and sodium copper chlorophyllin shows that these products have prominent water solubility and significantly-improved stability, and are easily transported and carried. However, there has been no report on the research of using natural porphin salts as plant growth regulators. Plant immunity inducers, also known as plant vaccines, are a class of novel biological pesticides developed based on the vaccine engineering technology in recent years. Plant immunity inducers can regulate the metabolism and growth of plants and activate the immune systems of plants to achieve the effective prevention and control of diseases for crops (disease prevention), improve the resistance of crops, and improve the yield and quality, which is harmless to humans and animals and does not pollute the environment. Therefore, plant immunity inducers have become a hot spot for the current research and production of biological pesticides. The plant immunity inducers that have been discovered are mainly plant or microorganism-derived small molecules such as salicylic acid and matrine, large molecules such as humic acid and le