US-20260124251-A1 - COMPOSITION FOR ENDOMETRIAL REGENERATION CONTAINING A UTERUS-DERIVED DECELLULARIZED EXTRACELLULAR MATRIX (UDECM) AND A MANUFACTURING METHOD THEREFOR

Abstract

The present invention relates to a composition for endometrial regeneration containing a uterus-derived decellularized extracellular matrix (UdECM) and a manufacturing method therefor. Characterized by containing uterus-derived decellularized extracellular matrix (UdECM), the composition can exhibit the effect of enhancing the regeneration, receptivity, and implantation capabilities of the endometrium.

Inventors

• Dong Woo Cho
• Jin Ah Jang
• Tugce SEN
• Jung Ho Ahn
• Dan Bi Lee
• Mi Hyeon BAE
• Youn Jung KANG

Assignees

• POSTECH Research and Business Development Foundation
• CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Dates

Publication Date

20260507

Application Date

20230615

Priority Date

20221013

Claims (15)

1. 1 . A composition for endometrial regeneration containing uterus-derived decellularized extracellular matrix (UdECM).
2. 2 . The composition of claim 1 , wherein the uterus is a uterus derived from a pig (porcine).
3. 3 . The composition of claim 1 , wherein the uterus-derived decellularized extracellular matrix is a whole-uterine decellularized extracellular matrix (Whole-UdECM) derived from the whole uterus, including the endometrium, myometrium, and perimetrium.
4. 4 . The composition of claim 1 , wherein the uterus-derived decellularized extracellular matrix is a decellularized extracellular matrix (Endo-UdECM) derived from the endometrium.
5. 5 . The composition of claim 1 , wherein the uterus-derived decellularized extracellular matrix (UdECM) is an Endo-UdECM derived from endometrium isolated from the whole uterus including endometrium, myometrium and perimetrium.
6. 6 . The composition of claim 1 , wherein the uterus-derived decellularized extracellular matrix is Endo-UdECM derived from endometrium separated from uterine tissue treated with surfactant after treating the surfactant on the whole uterus including endometrium, myometrium and perimetrium.
7. 7 . The composition of claim 1 , further comprising collagen, glycoprotein, and proteoglycan components.
8. 8 . The composition of claim 7 , wherein the composition increases the expression of the receptor of hormone when treated with a steroid hormone and has the function of promoting regeneration of the endometrium and angiogenesis.
9. 9 . A method for manufacturing a composition for endometrial regeneration, comprising the steps of: preparing a whole uterus, which includes the endometrium and myometrium; treating a prepared uterine tissue with a first surfactant; separating the endometrium from the uterine tissue treated with the first surfactant; and treating a separated endometrium with a second surfactant.
10. 10 . The method of claim 9 , wherein the first surfactant is sodium dodecyl sulfate (SDS).
11. 11 . The method of claim 9 , wherein the second surfactant is Triton X-100.
12. 12 . A bioink composition comprising the uterus-derived decellularized extracellular matrix (UdECM) according to claim 1 .
13. 13 . A composition for culturing uterus organoids, comprising the uterus-derived decellularized extracellular matrix (UdECM) according to claim 1 .
14. 14 . A composition for producing an organ on a chip, comprising the uterus-derived decellularized extracellular matrix (UdECM) according to claim 1 .
15. 15 . A method for regenerating endometrium in a subject in need thereof, comprising administering to the endometrium of the subject an effective amount of the composition according to claim 1 .

Description

TECHNICAL FIELD The present invention relates to a uterine tissue-specific extracellular matrix that can improve the regeneration, receptivity, and implantation abilities of the endometrial (endometrium), and particularly to a composition for endometrial regeneration, including a uterus-derived decellularized extracellular matrix (UdECM), and a manufacturing method therefor. BACKGROUND ARTS OF THE INVENTION The uterus is one of the main reproductive organs of women and is composed of several tissue layers (endometrium, myometrium, and serosa). Approximately 10% of cases of female infertility are related to problems with the health of the uterus. Healthy endometrial tissue is very important for successful embryo implantation and maintaining pregnancy. In particular, the thickness of the endometrium is one of the main characteristics that have been used as an indicator of endometrial receptivity. Many studies have reported that a sufficient thickness of the endometrium is essential for successful implantation and pregnancy. In other words, if the thickness of the endometrium is too thin (less than 7 mm), it becomes difficult to maintain pregnancy. For this reason, various treatment methods are being studied to improve the thickness of the endometrium, and despite various treatment strategies including hormone therapy, intrauterine injection of growth factors, and the use of vasoactive substances, it is still difficult to see clinically significant effects, and the regeneration of the endometrium remains a challenging aspect of female infertility treatment. To overcome these limitations, transplantation of individual extracellular matrix (ECM) components from specific tissues or biomaterials based on collagen or hyaluronic acid has been used to regenerate the endometrium. However, such single ECM-based biomaterials have the limitation of being unable to mimic tissue-specific extracellular matrices that support tissue-specific microenvironments and cell-to-cell interactions based on unique combinations of various proteins. RELATED TECHNICAL DOCUMENTS Patent Documents (Patent Document 1) Korean open-laid patent No. 10-2021-0018639 (Laid open Date: Feb. 18, 2021) SUMMARY OF THE INVENTION Technical Subject The present invention aims to provide a composition for endometrial regeneration that can improve the regeneration, receptivity, and implantation abilities of the endometrium to solve the above-mentioned problems. The present invention also aims to provide a composition for endometrial regeneration that contains collagens, glycoproteins, and proteoglycans, increases the expression of the receptors of the above hormones when treated with steroid hormones, and has the functions of endometrial regeneration and promotion of angiogenesis. In addition, the present invention aims to further improve the responsiveness to steroid hormones and to further enhance the receptivity of the endometrium. Technical Solution One exemplary embodiment of the present invention to achieve the above objectives may be a composition for endometrial regeneration comprising uterus-derived decellularized extracellular matrix (UdECM). Preferably, but not necessarily, the uterus may be a uterus derived from a pig. Preferably, but not necessarily, the uterus-derived decellularized extracellular matrix may be a whole-uterine decellularized extracellular matrix (Whole-UdECM) derived from the whole uterus, including the endometrium, myometrium, and perimetrium. Preferably, but not necessarily, the uterus-derived decellularized extracellular matrix may be a decellularized extracellular matrix (Endo-UdECM) derived from the endometrium. Preferably, but not necessarily, the uterus-derived decellularized extracellular matrix (UdECM) may be an Endo-UdECM derived from endometrium isolated from the whole uterus including endometrium, myometrium and perimetrium. Preferably, but not necessarily, the uterus-derived decellularized extracellular matrix may be Endo-UdECM derived from endometrium separated from uterine tissue treated with surfactant after treating the surfactant on the whole uterus including endometrium, myometrium and perimetrium. Preferably, but not necessarily, a composition for endometrial regeneration comprising whole-UdECM derived from the uterine tissue (Whole Uterus) may be preferably intended for patients with uterine synechiae. Preferably, but not necessarily, the composition for endometrial regeneration according to the present invention may include collagen, glycoprotein, and proteoglycan components. Preferably, but not necessarily, the composition for endometrial regeneration according to the present invention can increase the expression of the receptor of hormone when treated with a steroid hormone and has the function of promoting regeneration of the endometrium and angiogenesis. Another exemplary embodiment of the present invention to achieve the above objectives may be a manufacturing method of composition for endometrial regen