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US-20260125613-A1 - THERMAL HYDROLYSIS OF MICROALGAE SPECIES FOR THE PRODUCTION OF BIO-CRUDE AND OTHER BIOACTIVE MOLECULES

US20260125613A1US 20260125613 A1US20260125613 A1US 20260125613A1US-20260125613-A1

Abstract

Disclosed is a method for producing biocrude and bioactive substances from microalgae biomass. The method has the steps of mixing and stirring dry microalgae biomass with water and an acid catalyst, heating the mixture at high pressure at a temperature and time based on each individual species or processing the biomass mixture in a colloidal mill with solvent, separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration, washing the lysed algae cells and non-polar extracts with organic solvents, separating the organic solvent mixture, which has ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration, and separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil.

Inventors

  • Rehaan LULLA
  • Dhruv Manoj JAIN
  • Muhtasim Md. KHAN

Assignees

  • ALGREEN LIMITED

Dates

Publication Date
20260507
Application Date
20241107

Claims (20)

  1. 1 . A method for producing biocrude and bioactive substances from microalgae biomass, comprising the steps of: mixing and stirring dry microalgae biomass with water and an acid catalyst; heating the mixture at high pressure at a temperature and time based on each individual species; separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration; washing the lysed algae cells and non-polar extracts with organic solvents; separating the organic solvent mixture, which comprises ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration; and separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil.
  2. 2 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 1 , wherein the mixing of dry microalgae biomass with water is in a ratio of 1:5.
  3. 3 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 1 , wherein the acid catalyst includes but is not limited to hydrochloric acid and sulfuric acid in water in a ratio of 5:0.08.
  4. 4 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 1 , wherein the temperature and time during the heating of the mixture comprise 100-250°C. for 10-180 minutes.
  5. 5 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 4 , wherein the heating of the mixture takes up to a maximum of 60% of the volume of a pressure vessel's capacity.
  6. 6 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 1 , wherein the water-soluble extracts comprise proteins, amino acids, glycerin, and other bioactive molecules.
  7. 7 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 1 , wherein the organic solvents comprise ethanol, methanol, and n-hexane in a ratio of 1:1 to 6:1 depending on the moisture and liquid composition of microalgae.
  8. 8 . A thermal hydrolysis system of microalgae biomass for producing biocrude and bioactive substances, comprising: a high-pressure vessel; a vacuum filtration; and a rotary evaporator.
  9. 9 . The thermal hydrolysis system, according to claim 8 , wherein an extraction of dry microalgae biomass in an aqueous medium is conducted in the high-pressure vessel at 100-250°C. for 10-180 minutes based on each individual species to produce bio-oil and bioactive substances.
  10. 10 . The thermal hydrolysis system, according to claim 9 , wherein the bio-oil comprises 90% long-chain free fatty acids and the bioactive substances comprise glycerin and other bioactive molecules such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).
  11. 11 . The thermal hydrolysis system, according to claim 9 , wherein the extraction further produces molecules including but not limited to 2-Pyrrolidinone, 1-Piperidinecarboxamide, 1,4-Benzenedicarboxylic acid, bis(2-ethylhexyl) ester, 3-Methyl-1,2-cyclopentanedione, and 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione.
  12. 12 . A method for producing biocrude and bioactive substances from microalgae biomass, comprising the steps of: mixing and stirring dry microalgae biomass with water and an acid catalyst; processing the biomass mixture in a colloidal mill with solvent; separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration; washing the lysed algae cells and non-polar extracts with organic solvents; separating the organic solvent mixture, which comprises ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration; and separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil.
  13. 13 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 12 , wherein the mixing of dry microalgae biomass with water is in a ratio of 1:5.
  14. 14 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 12 , wherein the acid catalyst includes but is not limited to hydrochloric acid and sulfuric acid in water in a ratio of 5:0.08.
  15. 15 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 12 , wherein the processing of the biomass mixture with the solvent is optimum in a ratio of 1:2 to 1:7 depending on the lipid content of the microalgae and the moisture content.
  16. 16 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 12 , wherein the water-soluble extracts comprise proteins, amino acids, glycerin, and other bioactive molecules.
  17. 17 . The method for producing biocrude and bioactive substances from microalgae biomass, according to claim 12 , wherein the organic solvents comprise ethanol, methanol, and n-hexane in a ratio of 1:1 to 6:1 depending on the moisture and liquid composition of microalgae.
  18. 18 . A system of microalgae biomass for producing biocrude and bioactive substances, comprising: a colloidal mill; a vacuum filtration; a rotary evaporator; and a storage tank.
  19. 19 . The system, according to claim 18 , wherein the extraction of dry microalgae biomass in an aqueous medium is conducted in the colloidal mill to produce bio-oil and bioactive substances, wherein the bio-oil comprises 90% long-chain free fatty acids and the bioactive substances comprise glycerin and other bioactive molecules such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).
  20. 20 . The system, according to claim 19 , wherein the extraction further produces molecules including but not limited to 2-Pyrrolidinone, 1-Piperidinecarboxamide, 1,4-Benzenedicarboxylic acid, bis(2-ethylhexyl) ester, 3-Methyl-1,2-cyclopentanedione, and 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS Not applicable. FIELD OF THE INVENTION The present invention relates to a thermal hydrolysis method and system for microalgae species, in particular a method and system of thermal hydrolysis for producing bio-crude and other bioactive molecules for industry. BACKGROUND OF THE INVENTION It is known in the prior art that microalgae biomass could produce bio-crude for industry use. Therefore, it is crucial to produce such bio-oil from the dry microalgae biomass to produce biofuels. There are a few methods of producing said bio-crude from the microalgae biomass that are versatile, scalable, and energy efficient in recent work aiming to produce high-quality fuels. Although they could use a wide range of renewal feedstocks such as vegetable oils, animal fats and waste oil or can be scaled up or down depending on the production needs, the existing methods can be energy-extensive and require a high production cost. Therefore, there is a need to provide a simple method and system that involves low production costs to produce biocrude from microalgae biomass. WIPO Patent No. 2012062962 A1 discloses a method for recovering lipids from microbial biomass. The method comprises providing wet microbial biomass that contains lipids for extraction without disrupting the biomass cell walls, and subsequently, extracting said wet microbial biomass with a liquid extractant at an elevated temperature of at least 170° C. and elevated pressure. The combination of temperature and pressure is such that the lipids in the cells are contacted with said extractant. Subsequently, the extracted lipids are recovered from or with the extractant. Although the method of recovering lipids from microbial biomass could recover a wide range of renewable feedstocks, including fats, oils, waxes and etc., the process is complex and may require skilled operators to operate, which increases the operational costs. WIPO Patent No. 2013184317 A1 discloses a combined hydrothermal liquefaction (HTL) and catalytic hydrothermal gasification (CHG) system and process that convert various biomass-containing sources into separable bio-oils and aqueous effluents that contain residual organics. Bio-oils may be converted to useful bio-based fuels and other chemical feedstocks. Residual organics in HTL aqueous effluents may be gasified and converted into medium-BTU product gases and directly used for process heating or to provide energy. However, the system and process are complex and may require skilled operators to operate, which increases operational costs. WIPO Patent No. 2014022218 A1 discloses production of hydrocarbons from select coal and algal biomass mixtures. Hydrocarbons suitable for commercial use or for further refining are produced by a process that mixes an aliphatic-rich biomass and coal to obtain a feedstock. The feedstock is subjected to produce a product mixture and is further separated for bio-oil or hydrocarbons. However, the system and process are complex and may require skilled operators to operate, which increases operational costs. U.S. Pat. No. 10,077,454 B1 discloses a method and system configured to convert algal biomass into biofuels, alcohols, and/or other useful by-products. The method includes an integrated biochemical and thermochemical process that provides high-purity biofuels and mixed alcohols while minimizing waste and/or maximizing efficiency. Although the method and system could provide low-cost and high-efficiency production of biofuels from algal biomass sources, the method and system are complex and may require skilled operators to operate. Hence, there is a need to provide a simple and effective method and system of thermal hydrolysis for producing bio-crude and other bioactive molecules for the industry with a less complex process, less manpower, and low costs. SUMMARY OF THE INVENTION It is an objective of the present invention to provide an improved and simple method and system of thermal hydrolysis for producing bio-crude and other bioactive molecules that could be easily conducted at a low cost. Accordingly, these objectives may be achieved by following the teachings of the present invention. The present invention relates to a thermal hydrolysis method of microalgae biomass for producing biocrude and bioactive substances, comprising the steps of: mixing and stirring dry microalgae biomass with water and an acid catalyst, heating the mixture at high pressure at a temperature and time based on each individual species, separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration, washing the lysed algae cells and non-polar extracts with organic solvents, separating the organic solvent mixture, which comprises ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration, and separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil. BRIEF DESCRIPTION OF DRAWINGS The features