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US-20260125719-A1 - LIPID PRODUCTION CONTROL FACTOR FOR OLEAGINOUS YEAST

US20260125719A1US 20260125719 A1US20260125719 A1US 20260125719A1US-20260125719-A1

Abstract

Lipid production control factors in yeasts are provided. A yeast 115694 gene, a yeast 4888 gene, and a yeast 45559 gene are provided as lipid production control factors.

Inventors

  • Hiroaki Takaku
  • Harutake YAMAZAKI
  • Hideo Araki
  • Sachiyo ABURATANI
  • Katsuro Yaoi

Assignees

  • THE NIIGATA INSTITUTE OF SCIENCE AND TECHNOLOGY
  • FUJI OIL CO., LTD.

Dates

Publication Date
20260507
Application Date
20251113
Priority Date
20200228

Claims (6)

  1. 1 . A method for producing a yeast with a regulated lipid production capacity, the method comprising performing on a yeast at least one selected from the group consisting of the following (3): (3) regulating the expression and/or function of a yeast 4888 gene.
  2. 2 . The method according to claim 1 , producing a yeast with an increased lipid production capacity, the method comprising performing at least one selected from the group consisting of the following (3A): (3A) increasing the expression and/or function of the yeast 4888 gene.
  3. 3 . The method according to claim 1 , producing a yeast with an increased lipid production capacity, the method comprising performing at least one selected from the group consisting of the following (3Aa): (3 Aa) increasing the expression of the yeast 4888 gene.
  4. 4 . The method according to claim 1 , wherein the region between the transmembrane domain and the nuclear localization signal is an amino acid region at positions 1100 to 1200 from the N-terminus of the amino acid sequence of the yeast 115694 gene represented by SEQ ID NO: 1.
  5. 5 . The method according to claim 1 , wherein the yeast is an oleaginous yeast.
  6. 6 . The method according to claim 1 , wherein the yeast belongs to the genus Lipomyces.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS This application is a division of U.S. application Ser. No. 17/802,694, filed 26 Aug. 2022, which is a U.S. National Stage application of PCT/JP2021/003621 filed 2 Feb. 2021, which claims priority to Japanese Application No. 2020-032655 filed 28 Feb. 2020, the entire disclosures of which are hereby incorporated by reference in their entireties. SEQUENCE LISTING The present application contains a Sequence Listing which has been submitted electronically in XML format and is hereby incorporated by reference in its entirety. Said XML copy, created on 13 Nov. 2025, is named “P20-282US_Sequence_Listing.xml” and is 99,095 bytes in size. TECHNICAL FIELD The present invention relates to lipid production control factors of oleaginous yeasts. BACKGROUND ART Fat and oil are closely involved in our daily lives, and their applications are largely categorized into food and industrial use. Fat and oil used in food include salad oil for cooking, and processed fat and oil such as margarine and dressing prepared by processing fat-and-oil materials. Fat and oil used in industrial application include those directly used as fuels or lubrication oil, and fat-and-oil chemical products produced through chemical conversion, such as shampoos, conditioners, and cosmetics. The raw materials of major three fat and oil items (soybean oil, rapeseed oil, and palm oil) are vegetable oils obtained mostly by pressing seeds such as rapeseed and soybean, or the flesh of fruits such as palm and olive. Other countries can supply fat and oil by growing oilseed plants (raw materials of fat and oil). In Japan, however, there is a need to develop a fat and oil production system suitable for the nation in order to increase the self-sufficiency rate of edible fat and oil. Fat and oil production by using microorganisms has been a focus of attention these days, as a substitute for fat and oil production from oilseed plants. In particular, oleaginous yeasts such as yeasts of genus Lipomyces have a high fat-and-oil accumulation capacity. CITATION LIST Patent Literature PTL 1: JP2019-146543A Non-Patent Literature NPL 1: H. Yamazaki, S. Kobayashi, S. Ebina, S. Abe, S. Ara, Y. Shida, W. Ogasawara, K. Yaoi, H. Araki & H. Takaku: Appl. Microbiol. Biotechnol., 103, 6297 (2019). SUMMARY OF INVENTION Technical Problem PTL 1 and NPL 1 report that mutation processing was performed on yeasts of genus Lipomyces, and that yeasts with a higher lipid production capacity were screened. However, these studies identified no lipid production control factors. Once the factors are identified, the lipid production capacity of yeasts will be more freely regulated, and accumulation of the factors will enable the production of a yeast with a higher lipid production capacity. An object of the present invention is to provide lipid production control factors in yeasts. Solution to Problem The present inventors conducted extensive research and found that the yeast 115694 gene, yeast 4888 gene, and yeast 45559 gene are lipid production control factors; and further found that a lipid production capacity can be regulated by regulating the expression and/or function of these genes, or by expressing mutants of these genes. The inventors conducted further research on the basis of these findings and completed the invention. Specifically, the present invention includes the following subject matter. Item 1. A method for producing a yeast with a regulated lipid production capacity, the method comprising performing on a yeast at least one selected from the group consisting of the following (1), (2), (3), and (4): (1) regulating the expression and/or function of a yeast 115694 gene,(2) expressing the yeast 115694 gene having a mutation in a region between the transmembrane domain and the nuclear localization signal,(3) regulating the expression and/or function of a yeast 4888 gene, and(4) regulating the expression and/or function of a yeast 45559 gene. Item 2. The method according to Item 1, producing a yeast with an increased lipid production capacity, the method comprising performing at least one selected from the group consisting of the following (1A), (2), (3A), and (4A): (1A) increasing the expression and/or function of the yeast 115694 gene,(2) expressing the yeast 115694 gene having a mutation in a region between the transmembrane domain and the nuclear localization signal,(3A) increasing the expression and/or function of the yeast 4888 gene, and(4A) decreasing the expression and/or function of the yeast 45559 gene. Item 3. The method according to Item 1 or 2, producing a yeast with an increased lipid production capacity, the method comprising performing at least one selected from the group consisting of the following (1Aa), (2), (3Aa), and (4Aa): (1Aa) increasing the expression of the yeast 115694 gene,(2) expressing the yeast 115694 gene having a mutation in a region between the transmembrane domain and the nuclear localization signal,(3Aa) increas