US-20260125720-A1 - Method for Producing Lysophospholipid-Containing Composition and Method for Producing Oil-In-Water Emulsion Composition Using Same

Abstract

The present disclosure relates to a method for producing a lysophospholipid-containing composition in which a phospholipid is decomposed by phospholipase A.

Inventors

• Kenta SUSAKI
• Hiroyoshi Yamamoto
• Hideaki Kobayashi
• Ryosuke Hoshina
• Takuya Ishikawa

Assignees

• KEWPIE CORPORATION

Dates

Publication Date

20260507

Application Date

20230524

Priority Date

20221006

Claims (5)

1. 1 . A method for producing a lysophospholipid-containing composition, in which a phospholipid is decomposed by phospholipase A, the method comprising: a mixing step of uniformly mixing raw materials containing a phospholipid, a fat and oil, a polyhydric alcohol, and pure water, and phospholipase A to initiate enzymolysis of the phospholipid by the phospholipase A; a phospholipid enzymolysis step of subjecting the phospholipid to the enzymolysis by the phospholipase A such that a decomposition ratio of the phospholipid in a mixture obtained in the mixing step is 20% by mass or more; and an enzyme inactivation step of inactivating the phospholipase A in an enzymolysis product obtained in the phospholipid enzymolysis step, wherein a value calculated by a L2 value−a L1 value is positive in the obtained lysophospholipid-containing composition, the L1 value: a brightness value of the lysophospholipid-containing composition, the L2 value: a brightness value of an oil-in-water emulsion obtained by dispersing the lysophospholipid-containing composition in 100 times by mass of pure water, a total amount of the phospholipid, the fat and oil, the polyhydric alcohol, the pure water, and the phospholipase A used in the mixing step is 90% by mass or more with respect to a total amount of the raw materials and the phospholipase A used, a content of the polyhydric alcohol in the raw materials is 0.4 to 3.0 times by mass with respect to a content of the pure water in the raw material, a content of the phospholipid in the raw materials is 0.07 to 0.5 times by mass with respect to a total content of the phospholipid and the fat and oil in the raw material, and a total content of the polyhydric alcohol and the pure water in the raw materials is 0.25 to 4.0 times by mass with respect to the total content of the phospholipid and the fat and oil in the raw material.
2. 2 . The method for producing a lysophospholipid-containing composition according to claim 1 , wherein the value calculated by the L2 value−the L1 value is +5 or more.
3. 3 . A method for producing an oil-in-water emulsion composition, comprising: a water dispersion step of dispersing the lysophospholipid-containing composition obtained by method for producing a lysophospholipid-containing composition according to claim 1 in pure water.
4. 4 . A method for producing a mixed composition, comprising: a mixing step of mixing one or two or more of a fat and oil, a lipid-soluble substance, a polyhydric alcohol, pure water, and a water-soluble substance with the lysophospholipid-containing composition obtained by the method for producing a lysophospholipid-containing composition according to claim 1 , wherein a value calculated by a L2 value−a L1 value is positive in the mixed composition obtained by the mixing step, the L1 value: a brightness value of the mixed composition, the L2 value: a brightness value of an oil-in-water emulsion obtained by dispersing the mixed composition in 100 times by mass of pure water.
5. 5 . A method for producing an oil-in-water emulsion composition, comprising: a water dispersion step of dispersing the mixed composition obtained by the method for producing a mixed composition according to claim 4 in pure water.

Description

TECHNICAL FIELD The present invention relates to a method for producing a lysophospholipid-containing composition, in which a phospholipid is decomposed into a lysophospholipid by a novel method in which the enzymolysis of the phospholipid by phospholipase A proceeds without using an organic solvent accompanied by removal, and the lysophospholipid-containing composition to be obtained has self-emulsifying properties and is easily used for various applications, and a method for producing an oil-in-water emulsion composition using the lysophospholipid-containing composition. BACKGROUND ART As a method for decomposing a phospholipid by phospholipase A, for example, a method for dissolving a phospholipid in an organic solvent and bringing the phospholipid into contact with phospholipase A while mixing the organic solvent phase with a dispersion liquid of the phospholipase A (Non-Patent Literature 1), a method for preparing an oil-in-water emulsion using a phospholipid as an emulsifier, and bringing the phospholipase A dispersed in a water phase into contact with the phospholipid present at an interface between the oil phase and the water phase (Non-Patent Literature 2), and the like are known. However, in the method described in Non-Patent Literature 1, the enzyme reaction proceeds easily by using an organic solvent, but the resultant product cannot be used for various applications, for example, foods, pharmaceuticals, and the like, unless the organic solvent is removed. In particular, the manufacturing conditions using diethyl ether described in Non-Patent Literature 1 are not permitted for food applications due to safety. In addition, in the method described in Non-Patent Literature 2, the phospholipid can be enzymatically decomposed in a state of an oil-in-water emulsion without using an organic solvent, but there is a problem that the enzymolysis reaction rate is slower than that in a method using an organic solvent, and a large amount of energy is required to finally remove water. Any of these methods have some problems, and a new enzymolysis method in which these problems are solved has been required. CITATION LIST Non Patent Literature [Non-Patent Literature 1] Biochemical Experiment Course 3 “Chemistry of Lipids”, edited by the Japanese Biochemical Society, pp. 264 to 265, published on Nov. 25, 1974.[Non-Patent Literature 2] D. M. Cabezas, R. Madoery, B. W. K. Diehl, M. C. Tomás, Emulsifying Properties of Different Modified Sunflower Lecithins, J. Am. Oil Chem. Soc. (2012) 89: pp. 355-361. SUMMARY OF INVENTION Technical Problem An object of the present invention is to provide a method for producing a lysophospholipid-containing composition, in which a phospholipid is decomposed into a lysophospholipid by a novel method in which the enzymolysis of the phospholipid by phospholipase A proceeds without using an organic solvent accompanied by removal, and the lysophospholipid-containing composition to be obtained has self-emulsifying properties and is easily used for various applications, and a method for producing an oil-in-water emulsion composition using the lysophospholipid-containing composition. Solution to Problem The present inventors have conducted intensive studies to achieve the above object. As a result, it was found that, in a case of attempting a novel enzymolysis in which the phospholipid is subjected to enzymolysis by phospholipase A in a polyhydric alcohol-based mixture containing a phospholipid, a fat and oil, a polyhydric alcohol, and a small amount of pure water as raw materials, and the mixing ratio of the raw materials of the mixture is controlled such that the obtained composition has self-emulsifying properties, the enzymolysis of the phospholipid by phospholipase A proceeds without using an organic solvent accompanied by removal, and a lysophospholipid-containing composition having self-emulsifying properties and being easily used for various applications is obtained, thereby completing the present invention. That is, present invention relates to (1) a method for producing a lysophospholipid-containing composition, in which the lysophospholipid-containing composition obtained by decomposing a phospholipid with phospholipase A, the method including: a mixing step of uniformly mixing raw materials containing a phospholipid, a fat and oil, a polyhydric alcohol, and pure water, and phospholipase A to initiate enzymolysis of the phospholipid by the phospholipase A;a phospholipid enzymolysis step subjecting the phospholipid to the enzymolysis by the phospholipase A such that a decomposition ratio of the phospholipid in a mixture obtained in the mixing step is 20% by mass or more; andan enzyme inactivation step inactivating the phospholipase A in an enzymolysis product obtained in the phospholipid enzymolysis step,in which a value calculated by a L2 value−a L1 value is positive in the obtained lysophospholipid-containing composition,the L1 value: a brightness value of the lysophospholipid-c