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US-20260125721-A1 - ACIDOPHILIC FUSARIUM OXYSPORUM STRAINS, METHODS OF THEIR PRODUCTION AND METHODS OF THEIR USE

US20260125721A1US 20260125721 A1US20260125721 A1US 20260125721A1US-20260125721-A1

Abstract

The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.

Inventors

  • Mark A. Kozubal
  • Richard E. MACUR
  • William P. INSKEEP

Assignees

  • THE FYNDER GROUP, INC.

Dates

Publication Date
20260507
Application Date
20250707

Claims (20)

  1. 1 - 20 . (canceled)
  2. 21 . A genetically modified fungus of the strain MK7, deposited as ATCC Accession Deposit No. PTA-10698.
  3. 22 . The fungus of claim 21 , wherein the fungus comprises a genetic modification that reduces expression of an endogenous gene.
  4. 23 . The fungus of claim 22 , wherein the endogenous gene encodes an enzyme.
  5. 24 . The fungus of claim 23 , wherein the enzyme functions in a metabolic pathway for a metabolite.
  6. 25 . The fungus of claim 24 , wherein the metabolite is a mycotoxin.
  7. 26 . The fungus of claim 25 , wherein the mycotoxin is selected from the group consisting of fumonisin, bikaverin, enniatin, fusaric acid, lycomarasmin, moniliformin, oxysporone, trichothecene, zearelone, naphthoquinone, anthraquinone, nonaketide naphthazarin quinone, norbikaverin, heptaketide, nectriafurone, 5-O-methyljavanicin, anhydrofusarubin lactol, 4-Acetoxyscirpenediol, 4-3-acetoxy-3,15-dihydroxy-12,13-epoxytrichothec-9-ene, monodeacetylanguidin, 4-acetylscirpentriol, 15-acetylscirpentriol, 3-Acetyldeoxynivalenol, Deoxynivalenol monoacetate, 3″-acetoxy-7″,15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one, 8-Acetylneosolaniol, Neosolaniol monoacetate, 15-triacetoxy-3″-hydroxy-1 2,13-epoxytrichothec-9-ene, 4-Acetoxyscirpenediol, Acetyl T-2 toxin, 3″,4″,15-triacetoxy-8″-(3-methylbutyry(oxy)-1 2,1 3-epoxytricho-thec-9-ene), Anguidin, Diacetoxyscirpenol, Avenacein, Beauvericin, Butenolide, 4-acetamido-4-hydroxy-2-butenoic-acid-lactone, Calonectrin, 3″,15-diacetoxy-12,13-epoxytrichothec-9-ene, 15-Deacetylcalonectrin, 15-De-O-acetylcalonectrin, 3″-acetoxy-1 5-hydroxy-12, 13-epoxytrichothec-9-ene, Deoxynivalenol, Rd toxin, Vomitoxin, 3″,7″,15-trihydroxy-12,13-epoxytricho-thec-9-en-8-one, Deoxynivalenol diacetate, Diacetyldeoxynivalenol, Deoxynivalenol monoacetate 3-Acetyldeoxynjvalenol, Diacetoxyscirpendiol, 7″-Hydroxydiacetoxyscirpenol, Diacetoxyscirpenol, 4,15-diacetoxy-3′-hydroxy12,13-epoxytrjchothec-9-ene, Diacetoxyscerpentriol, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetyldeoxynivalenol, Deoxynivalenol diacetate, 3″,15-diacetoxy-7-hydroxy 12,13-epoxytrichothec-9-en-8-one, Diacetylnivalenol, Nivalenol diacetate, 4,15-diacetoxy-3′,7′-dihydroxy-12,13-epoxytrichothec-9-en-8-one, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetoxyscirpentriol, 4,15-diacetoxy-3″,7″,8″-trihydroxy-12,13-epoxytrichothec-9-ene, Enniatins, Fructigenin, Fumonisin B1, 1,2,3-propanetricarboxylic acid 1,-1-[1-(12-amino-4,9,11-trihydroxy-2-methyltridecyl)-2-(1-methylpentyl)-1,2-ethanediyI]ester, macrofusine, Fusarenon-X, Fusarenon, Monoacetylnivalenol, Nivalenol monoacetate, 4-acetoxy-3″,7″,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one, Fusaric acid, Fusarinic acid, 5-butylpicolinic acid, F-2 toxin, Zearalenone, HT-2 toxin, 15-acetoxy-3″,4-dihydroxy-8″-(3-methylbutyryloxy)-12-epoxytricho-thec-9-ene, 7″-Hydroxy-diacetoxyscirpenol, Diacetoxyscirpendiol, 4,15-diacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, 8″-Hydroxydiacetoxyscirpenol, Neosolaniol, 1,4-Ipomeadiol, 1-(3-furyI)-1,4-pentanediol, Ipomeanine, 1-(3-furyl)-1,4-pentanetione, 1-Ipomeanol, 1-(3-furyl)-1-hydroxy-4-pentanone, 4-ipomeanol, 1-(3-furyl)-4-hydroxy4pentanone, Lateritin, Lycomarasmin, Moniliformin, (potassium salt of 1-hydroxycyclobut-1-ene-3,4-dione, sodium salt of 1-hydroxycyclobut-1-ene-3,4-dione, Monoacetoxyscirpenol, 15-acetoxy-3″,4″-dihydroxy-12,13-epoxytrichothec-9ene, Monoacetylnivalenol, Monodeacetylanguidin, 4-Acetoxyscirpenediol, Neosolaniol, 8″-Hydroxydiacetoxyscirpenol, 4,15-diacetoxy-3″8″-dihydroxy-12,13-epoxytrichothec-9-ene, Neosolaniolacetate, 8-Acetylneosolaniol, Neosolaniol monoacetate, 8-Acetylneosolaniol, Nivalenol, 3″,4″,7″,15″-tetrahydroxy-12,13-epoxy-trichothec-9-en-8-one, Nivalenol diacetate, Diacetylnivalenol, Nivalenol monoacetate, NT-1 toxin, T-1 toxin, 4″,8″-diacetoxy-3″,15-dihydroxy-12,13-epoxy-trichothec-9-ene, NT-2 toxin, 4″-acetoxy-3″, 8″,15-trihydroxy-12,13-epoxytrichothec-9-ene, Deoxynivalenol, Sambucynin, Scirpentriol, 3″,4″,15″-trihydroxy-12,13-epoxytrichothec-9-ene, Solaniol, Neosolaniol, T-2 toxin, 4″,15″-diacetoxy-3″-hydroxy-8″-(3-methylbutyrlyloxy)-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpendiol, 4″,8″,15″-triacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpenol, 3″, 4″,15″-triacetoxy-12,13-epoxytrichothec-9-ene, Vomitoxin, Deoxynivalenol, Yavanicin, Zearalenol, 2,4-dihydroxy-6-(6,10-dihydroxy-trans-1-undecenyl)-benzoic acid-lactone, Zearalenone, and 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-resorcylic acid lactone.
  8. 27 . The fungus of claim 26 , wherein the mycotoxin is a fumonisin.
  9. 28 . The fungus of claim 27 , wherein the fumonisin is fumonisin Bl.
  10. 29 . The fungus of claim 2 , wherein the genetic modification is selected from the group consisting of a nucleotide substitution, a nucleotide deletion, a nucleotide insertion, and combinations thereof.
  11. 30 . The fungus of claim 29 , wherein the genetic modification is a nucleotide deletion.
  12. 31 . The fungus of claim 29 , wherein the genetic modification is a nucleotide substitution.
  13. 32 . The fungus of claim 29 , wherein the endogenous gene encodes an enzyme.
  14. 33 . The fungus of claim 32 , wherein the enzyme functions in a metabolic pathway for a metabolite.
  15. 34 . The fungus of claim 33 , wherein the metabolite is a mycotoxin.
  16. 35 . The fungus of claim 34 , wherein the mycotoxin is selected from the group consisting of fumonisin, bikaverin, enniatin, fusaric acid, lycomarasmin, moniliformin, oxysporone, trichothecene, zearelone, naphthoquinone, anthraquinone, nonaketide naphthazarin quinone, norbikaverin, heptaketide, nectriafurone, 5-O-methyljavanicin, anhydrofusarubin lactol, 4-Acetoxyscirpenediol, 4-3-acetoxy-3,15-dihydroxy-12,13-epoxytrichothec-9-ene, monodeacetylanguidin, 4-acetylscirpentriol, 15-acetylscirpentriol, 3-Acetyldeoxynivalenol, Deoxynivalenol monoacetate, 3″-acetoxy- 7 ″,15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one, 8-Acetylneosolaniol, Neosolaniol monoacetate, 15-triacetoxy-3″-hydroxy-1 2,13-epoxytrichothec-9-ene, 4-Acetoxyscirpenediol, Acetyl T-2 toxin, 3″,4″,15-triacetoxy-8″-(3-methylbutyry(oxy)-1 2,1 3-epoxytricho-thec-9-ene), Anguidin, Diacetoxyscirpenol, Avenacein, Beauvericin, Butenolide, 4-acetamido-4-hydroxy-2-butenoic-acid-lactone, Calonectrin, 3″,15-diacetoxy-12,13-epoxytrichothec-9-ene, 15-Deacetylcalonectrin, 15-De-O-acetylcalonectrin, 3″-acetoxy-1 5-hydroxy-12, 13-epoxytrichothec-9-ene, Deoxynivalenol, Rd toxin, Vomitoxin, 3″,7″,15-trihydroxy-12,13-epoxytricho-thec-9-en-8-one, Deoxynivalenol diacetate, Diacetyldeoxynivalenol, Deoxynivalenol monoacetate 3-Acetyldeoxynjvalenol, Diacetoxyscirpendiol, 7″-Hydroxydiacetoxyscirpenol, Diacetoxyscirpenol, 4,15-diacetoxy-3′-hydroxy12,13-epoxytrjchothec-9-ene, Diacetoxyscerpentriol, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetyldeoxynivalenol, Deoxynivalenol diacetate, 3″,15-diacetoxy-7-hydroxy 12,13-epoxytrichothec-9-en-8-one, Diacetylnivalenol, Nivalenol diacetate, 4,15-diacetoxy-3′,7′-dihydroxy-12,13-epoxytrichothec-9-en-8-one, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetoxyscirpentriol, 4,15-diacetoxy-3″,7″,8″-trihydroxy-12,13-epoxytrichothec-9-ene, Enniatins, Fructigenin, Fumonisin B1, 1,2,3-propanetricarboxylic acid 1,-1-[1-(12-amino-4,9,11-trihydroxy-2-methyltridecyl)-2-(1-methylpentyl)-1,2-ethanediyI]ester, macrofusine, Fusarenon-X, Fusarenon, Monoacetylnivalenol, Nivalenol monoacetate, 4-acetoxy-3″,7″,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one, Fusaric acid, Fusarinic acid, 5-butylpicolinic acid, F-2 toxin, Zearalenone, HT-2 toxin, 15-acetoxy-3″,4-dihydroxy-8″-(3-methylbutyryloxy)-12-epoxytricho-thec-9-ene, 7″-Hydroxy-diacetoxyscirpenol, Diacetoxyscirpendiol, 4,15-diacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, 8″-Hydroxydiacetoxyscirpenol, Neosolaniol, 1,4-Ipomeadiol, 1-(3-furyI)-1,4-pentanediol, Ipomeanine, 1-(3-furyl)-1,4-pentanetione, 1-Ipomeanol, 1-(3-furyl)-1-hydroxy-4-pentanone, 4-ipomeanol, 1-(3-furyl)-4-hydroxy4pentanone, Lateritin, Lycomarasmin, Moniliformin, (potassium salt of 1-hydroxycyclobut-1-ene-3,4-dione, sodium salt of 1-hydroxycyclobut-1-ene-3,4-dione, Monoacetoxyscirpenol, 15-acetoxy-3″,4″-dihydroxy-12,13-epoxytrichothec-9ene, Monoacetylnivalenol, Monodeacetylanguidin, 4-Acetoxyscirpenediol, Neosolaniol, 8″-Hydroxydiacetoxyscirpenol, 4,15-diacetoxy-3″8″-dihydroxy-12,13-epoxytrichothec-9-ene, Neosolaniolacetate, 8-Acetylneosolaniol, Neosolaniol monoacetate, 8-Acetylneosolaniol, Nivalenol, 3″,4″,7″,15″-tetrahydroxy-12,13-epoxy-trichothec-9-en-8-one, Nivalenol diacetate, Diacetylnivalenol, Nivalenol monoacetate, NT-1 toxin, T-1 toxin, 4″,8″-diacetoxy-3″,15-dihydroxy-12,13-epoxy-trichothec-9-ene, NT-2 toxin, 4″-acetoxy-3″, 8″,15-trihydroxy-12,13-epoxytrichothec-9-ene, Deoxynivalenol, Sambucynin, Scirpentriol, 3″,4″,15″-trihydroxy-12,13-epoxytrichothec-9-ene, Solaniol, Neosolaniol, T-2 toxin, 4″,15″-diacetoxy-3″-hydroxy-8″-(3-methylbutyrlyloxy)-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpendiol, 4″,8″,15″-triacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpenol, 3″, 4″,15″-triacetoxy-12,13-epoxytrichothec-9-ene, Vomitoxin, Deoxynivalenol, Yavanicin, Zearalenol, 2,4-dihydroxy-6-(6,10-dihydroxy-trans-1-undecenyl)-benzoic acid-lactone, Zearalenone, and 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-resorcylic acid lactone.
  17. 36 . The fungus of claim 35 , wherein the mycotoxin is a fumonisin.
  18. 37 . The fungus of claim 36 , wherein the fumonisin is fumonisin Bl.
  19. 38 . A genetically modified fungus, wherein: the fungus is of the strain MK7, deposited as ATCC Accession Deposit No. PTA-10698; and the fungus comprises a nucleotide substitution and/or a nucleotide deletion that reduces expression of an endogenous gene, and wherein the endogenous gene encodes an enzyme that functions in a metabolic pathway for a mycotoxin selected from the group consisting of fumonisin, bikaverin, enniatin, fusaric acid, lycomarasmin, moniliformin, oxysporone, trichothecene, zearelone, naphthoquinone, anthraquinone, nonaketide naphthazarin quinone, norbikaverin, heptaketide, nectriafurone, 5-O-methyljavanicin, anhydrofusarubin lactol, 4-Acetoxyscirpenediol, 4-3-acetoxy-3,15-dihydroxy-12,13-epoxytrichothec-9-ene, monodeacetylanguidin, 4-acetylscirpentriol, 15-acetylscirpentriol, 3-Acetyldeoxynivalenol, Deoxynivalenol monoacetate, 3″-acetoxy-7″,15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one, 8-Acetylneosolaniol, Neosolaniol monoacetate, 15-triacetoxy-3″-hydroxy-1 2,13-epoxytrichothec-9-ene, 4-Acetoxyscirpenediol, Acetyl T-2 toxin, 3″,4″,15-triacetoxy-8″-(3-methylbutyry(oxy)-1 2,1 3-epoxytricho-thec-9-ene), Anguidin, Diacetoxyscirpenol, Avenacein, Beauvericin, Butenolide, 4-acetamido-4-hydroxy-2-butenoic-acid-lactone, Calonectrin, 3″,15-diacetoxy-12,13-epoxytrichothec-9-ene, 15-Deacetylcalonectrin, 15-De-O-acetylcalonectrin, 3″-acetoxy-1 5-hydroxy-12, 13-epoxytrichothec-9-ene, Deoxynivalenol, Rd toxin, Vomitoxin, 3″,7″,15-trihydroxy-12,13-epoxytricho-thec-9-en-8-one, Deoxynivalenol diacetate, Diacetyldeoxynivalenol, Deoxynivalenol monoacetate 3-Acetyldeoxynjvalenol, Diacetoxyscirpendiol, 7″-Hydroxydiacetoxyscirpenol, Diacetoxyscirpenol, 4,15-diacetoxy-3′-hydroxy12,13-epoxytrjchothec-9-ene, Diacetoxyscerpentriol, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetyldeoxynivalenol, Deoxynivalenol diacetate, 3″,15-diacetoxy-7-hydroxy 12,13-epoxytrichothec-9-en-8-one, Diacetylnivalenol, Nivalenol diacetate, 4,15-diacetoxy-3′,7′-dihydroxy-12,13-epoxytrichothec-9-en-8-one, 7″,8″-Dihydroxydiacetoxyscirpenol, Diacetoxyscirpentriol, 4,15-diacetoxy-3″,7″,8″-trihydroxy-12,13-epoxytrichothec-9-ene, Enniatins, Fructigenin, Fumonisin B1, 1,2,3-propanetricarboxylic acid 1,-1-[1-(12-amino-4,9,11-trihydroxy-2-methyltridecyl)-2-(1-methylpentyl)-1,2-ethanediyI]ester, macrofusine, Fusarenon-X, Fusarenon, Monoacetylnivalenol, Nivalenol monoacetate, 4-acetoxy-3″,7″,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one, Fusaric acid, Fusarinic acid, 5-butylpicolinic acid, F-2 toxin, Zearalenone, HT-2 toxin, 15-acetoxy-3″,4-dihydroxy-8″-(3-methylbutyryloxy)-12-epoxytricho-thec-9-ene, 7″-Hydroxy-diacetoxyscirpenol, Diacetoxyscirpendiol, 4,15-diacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, 8″-Hydroxydiacetoxyscirpenol, Neosolaniol, 1,4-Ipomeadiol, 1-(3-furyI)-1,4-pentanediol, Ipomeanine, 1-(3-furyl)-1,4-pentanetione, 1-Ipomeanol, 1-(3-furyl)-1-hydroxy-4-pentanone, 4-ipomeanol, 1-(3-furyl)-4-hydroxy4pentanone, Lateritin, Lycomarasmin, Moniliformin, (potassium salt of 1-hydroxycyclobut-1-ene-3,4-dione, sodium salt of 1-hydroxycyclobut-1-ene-3,4-dione, Monoacetoxyscirpenol, 15-acetoxy-3″,4″-dihydroxy-12,13-epoxytrichothec-9ene, Monoacetylnivalenol, Monodeacetylanguidin, 4-Acetoxyscirpenediol, Neosolaniol, 8″-Hydroxydiacetoxyscirpenol, 4,15-diacetoxy-3″8″-dihydroxy-12,13-epoxytrichothec-9-ene, Neosolaniolacetate, 8-Acetylneosolaniol, Neosolaniol monoacetate, 8-Acetylneosolaniol, Nivalenol, 3″,4″,7″,15″-tetrahydroxy-12,13-epoxy-trichothec-9-en-8-one, Nivalenol diacetate, Diacetylnivalenol, Nivalenol monoacetate, NT-1 toxin, T-1 toxin, 4″,8″-diacetoxy-3″,15-dihydroxy-12,13-epoxy-trichothec-9-ene, NT-2 toxin, 4″-acetoxy-3″, 8″,15-trihydroxy-12,13-epoxytrichothec-9-ene, Deoxynivalenol, Sambucynin, Scirpentriol, 3″,4″,15″-trihydroxy-12,13-epoxytrichothec-9-ene, Solaniol, Neosolaniol, T-2 toxin, 4″,15″-diacetoxy-3″-hydroxy-8″-(3-methylbutyrlyloxy)-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpendiol, 4″,8″,15″-triacetoxy-3″,7″-dihydroxy-12,13-epoxytrichothec-9-ene, Triacetoxy-scirpenol, 3″, 4″,15″-triacetoxy-12,13-epoxytrichothec-9-ene, Vomitoxin, Deoxynivalenol, Yavanicin, Zearalenol, 2,4-dihydroxy-6-(6,10-dihydroxy-trans-1-undecenyl)-benzoic acid-lactone, Zearalenone, and 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-resorcylic acid lactone.
  20. 39 . The fungus of claim 38 , wherein the mycotoxin is a fumonisin.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. provisional application No. 62/020,607, filed on Jul. 3, 2014, and U.S. provisional application 62/061,076, filed on Oct. 7, 2014, each of which is hereby incorporated by reference in its entirety for all purposes. DESCRIPTION OF THE TEXT FILE SUBMITTED ELECTRONICALLY The contents of the text file submitted electronically herewith are incorporated herein by reference in their entirety; A computer readable format copy of the Sequence Listing (filename: MONT_151_03US_ST25.txt, date recorded: Jul. 1, 2015, file size 2 kilobytes). TECHNICAL FIELD This application relates to novel isolated fungal strains of acidophilic Fusarium oxysporum, (e.g., MK7), their progeny, methods of producing such isolated fungal strains and their progeny, and methods of using such isolated fungal strains and their progeny to produce useful processes and products. For example, the isolated fungal strains and their progeny are useful for converting ligno-cellulosic feedstocks, algal biomass, and glycerol into energy-rich metabolites for bioenergy, for producing enzymes, antibiotics, specifically fatty acids and lipids (e.g. waxes) for commercial applications, dewaxing straw and for neutralizing acid and binding heavy metals. BACKGROUND Fusarium (synonyms: Fusisporium, Pseudofusarium, Sporotrichella) is a large genus of filamentous fungi widely distributed in soil and in association with plants. Most species are harmless saprobes and are relatively abundant members of the soil microbial community. Some species produce mycotoxins in cereal crops that can affect human and animal health if they enter the food chain. The main toxins produced by these Fusarium species are fumonisins and trichothecenes. Fusarium strains isolated from nature have proven useful to mankind in a wide variety of technologies, including for use as food and for the production of antibiotics. The need still exists for new isolates of Fusarium for use in new and existing applications. The present invention provides a unique isolated strain of Fusarium and its progeny which are useful for a number of different purposes, including but not limited to bioenergy production, as biolubricants, for biorecovery of precious metals by biosorption, for remediation of mine waste, for dewaxing of wheat straw, for degradation of algal biomass, and glycerol containing waste products, and for antibiotic, siderphore, and plasticizer production. SUMMARY The present invention provides isolated fungal strains of acidophilic Fusarium oxysporum, such as the isolated strain designated as MK7, which has been deposited as ATCC Deposit No. PTA-10698, and/or its progeny. “Progeny” as used herein refers to any and all descendants by lineage which originate from the isolated strain no matter however or wherever produced. Included within the definition of “progeny” as used herein are any and all mutants of the isolated/deposited strain and its progeny, wherein such mutants have all of the physiological and morphological characteristics of the isolated/deposited strain and its progeny. In one embodiment, the present invention provides an isolated ligno-cellulose degrading acidophilic fungal strain of Fusarium oxysporum or its progeny, wherein the isolated fungal strain of Fusarium oxysporum has at least the following indentifying characteristics: a) the isolated strain is acidophilic and can grow at a pH ranging from about 0.7 to about 7.5; andb) ability to produce lipids from ligno-cellulosic feedstocks, carbon containing waste products, carbohydrates, or a combination thereof under aerobic or substantially aerobic conditions; In one embodiment, the isolated strain of the present invention further comprises one or more the following additional identifying characteristics: c) ability to produce ethanol and/or hydrogen from ligno-cellulosic feedstocks, carbon containing waste products, carbohydrates, or a combination thereof under anaerobic or substantially anaerobic conditions;d) ability to tolerate Mn concentrations of up to 25 mM, As concentrations up to 250 mM or 300 mM, and Hg concentrations of up to 100 mM;e) ability to dewax wheat straw and other plant material;f) ability to produce lipids from algal feedstocks and from waste generated during biofuel production (e.g. processed algal biomass, glycerol) under aerobic or substantially aerobic conditions;g) ability to produce ethanol and/or hydrogen from algal feedstocks and from waste generated during biofuel production (e.g. processed algal biomass, glycerol) under anaerobic or substantially anaerobic conditions;h) ability to produce Diisooctyl phthalate (DIOP);i) ability to produce Hexanedioic acid, mono(2-ethylhexyl) ester; andj) comprising an 18S rRNA and ITS region DNA sequence that shares at least 98% identity to SEQ ID NO. 1. In some embodiments, the ligno-cellulosic feedstocks utilized by the isolated strain and its progeny are selected from the group cons