METHOD FOR SELECTIVELY PREPARING D-TAGATOSE-1,6-BISPHOSPHATE OR D-TAGATOSE-1-PHOSPHATE, AND USES THEREOF, IN PARTICULAR FOR PREPARING D-TAGATOSE

Abstract

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

Inventors

PAULAT, Léo
GUERARD HELAINE, Christine
HELAINE, Virgil
LEMAIRE, MARIELLE
De Berardinis, Véronique
PETIT, JEAN-LOUIS

Assignees

UNIVERSITÉ CLERMONT AUVERGNE
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Commissariat à l'Energie Atomique et aux Energies Alternatives
UNIVERSITÉ D'EVRY VAL D'ESSONNE

Dates

Publication Date: 20260507
Application Date: 20250930
Priority Date: 20241002