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WO-2026094581-A1 - MICROSCOPE SYSTEM

WO2026094581A1WO 2026094581 A1WO2026094581 A1WO 2026094581A1WO-2026094581-A1

Abstract

A microscope system 1 comprises a microscope 100 and a control device 200 that controls the microscope 100. The microscope 100 comprises: a gel immersion objective lens 130 which has a gel attached to the tip thereof; a stage 110 on which a sample is placed; and a revolver 120 which changes the distance in the optical axis direction between the position of the gel immersion objective lens 130 and the stage 110. The control device 200 sets a return speed at which the revolver 120 causes the gel immersion objective lens 130 to approach the stage 110 to be slower during a proximity period, in which the distance in the direction of the optical axis between the position of the gel immersion objective lens 130 and the stage 110 is equal to or less than a prescribed value, than during a period other than the proximity period.

Inventors

  • ASANO Madoka
  • KIUCHI Rie
  • OSAWA KENGO

Assignees

  • 株式会社エビデント

Dates

Publication Date
20260507
Application Date
20251008
Priority Date
20241028

Claims (5)

  1. A microscope comprising: a gel immersion objective lens with a gel attached to its tip; a stage on which a sample is placed; and a focusing device that changes the distance in the direction of the optical axis between the position of the gel immersion objective lens and the stage; The system comprises a control device for controlling the microscope, The control device is characterized in that it slows down the return speed at which the focusing device brings the gel immersion objective lens closer to the stage during the proximity period when the distance is below a predetermined value, compared to outside the proximity period.
  2. In the microscope system according to claim 1, The control device is characterized by determining the predetermined value according to the gel immersion objective lens.
  3. In the microscope system according to claim 2, The aforementioned microscope further, A plurality of objective lenses, including the aforementioned gel immersion objective lens, The system includes a plurality of mounting holes into which the plurality of objective lenses are attached, and a switching unit that switches between the objective lenses arranged on the optical path from among the plurality of objective lenses, The microscope system is characterized in that, when the control device determines that the gel immersion objective lens is positioned on the optical path by the switching unit, it determines the predetermined value based on the value of a parameter stored in association with the gel immersion objective lens.
  4. In the microscope system according to claim 1 or claim 2, The control device is characterized in that the retraction speed at which the focusing device moves the gel immersion objective lens away from the stage is faster than the return speed during the proximity period.
  5. In the microscope system according to claim 1 or claim 2, The aforementioned stage is a stage that moves the sample in a direction perpendicular to the optical axis, The control device is characterized in that the movement speed at which the stage moves the sample in a direction perpendicular to the optical axis is slower in a contact state where the gel is in contact with the sample or the sample holder containing the sample than in a non-contact state where the gel is not in contact with the sample or the sample holder.

Description

Microscope system This disclosure relates to a microscope system. Microscope objective lenses that use a gel instead of an immersion liquid such as water or oil, and which have a gel attached to the tip of the lens (hereinafter referred to as gel immersion objective lenses), offer superior maintainability and workability compared to liquid immersion objective lenses that use an immersion liquid, and are expected to improve the efficiency of microscopic observation. Such gel immersion objective lenses are described, for example, in Patent Document 1. This figure illustrates the configuration of a microscope system according to one embodiment.This diagram illustrates the configuration of a gel immersion objective lens.This is an example diagram showing a map image.This is an example of a table showing the specifications of the objective lens.This is an example of a table showing the settings for each mounting hole of a revolving nosepiece.This is a diagram illustrating the focusing mechanism.This is a diagram illustrating the process of removing air bubbles.This diagram illustrates the state of the gel when no field of view defects occur.This diagram illustrates the state of the gel when a field of view defect occurs.This diagram shows the relationship between the field of view and the XY range of motion.This is an example of a map image displaying the XY range of motion.This is a flowchart of the process according to the first embodiment.This is a flowchart illustrating an example of stage transition processing.This flowchart shows an example of the objective lens return process.This is a flowchart showing an example of a bubble removal process.This diagram illustrates the history of bubble detection processing results.This is a flowchart showing an example of the notification decision process.This diagram illustrates the retraction and return operation of the objective lens when a field of view movement is instructed on the application window.This is an example of how the map image is displayed before the view is moved, when the view is moved from within the application window.This is an example of how the map image is displayed after the field of view has been moved, when the field of view is moved via an instruction on the application window.This is another example of how the map image will be displayed after the field of view has been moved, when the field of view is moved via the application window.This diagram illustrates the retraction and return operation of the objective lens when a field of view movement is instructed using the JOG controller.This is an example of the map image displayed just before the objective lens retracts when the JOG controller is used to instruct the viewfinder to move.This is an example of the map image displayed immediately after the objective lens returns to its original position when the field of view is moved using the JOG controller.This is a flowchart of the process according to the second embodiment.This is a flowchart of the process according to the third embodiment.This is an example of how a map image is displayed when generating a stitched image.This is an example of how a map image is displayed when generating a stitched image.This is an example of how a map image is displayed when generating a stitched image.This is a flowchart of the process according to the fourth embodiment.This is a flowchart for determining the shooting order.This diagram illustrates the size of the continuous shooting range.This diagram illustrates the minimum number of evacuation steps required.This diagram explains the ground contact point and the shooting point.This diagram illustrates the sequence of movement of the point of contact.This diagram illustrates the order in which the shooting points move within each continuous shooting range.This diagram illustrates the order in which shooting points move within the target area.This is a flowchart of the process according to the fifth embodiment.This is a flowchart of the process according to the sixth embodiment.This diagram illustrates the hardware configuration of a computer used to implement a control device. Figure 1 illustrates the configuration of a microscope system according to one embodiment. Figure 2 illustrates the configuration of a gel immersion objective lens. Figure 3 illustrates a map image. The microscope system 1 will be described below with reference to Figures 1 through 3. The microscope system 1 shown in Figure 1 comprises a microscope 100 having a gel immersion objective lens 130 and a control device 200 for controlling the microscope 100. As shown in Figure 1, the microscope system 1 may also include a plurality of drive controllers (drive controllers 301, drive controllers 302) that control each motorized part of the microscope 100 according to instructions from the control device 200. Furthermore, it may include a display device 401, an input device 402, and a jog controller 403 connected to the control device 20