WO-2026095307-A1 - ORAL MICROBIOME INSPECTION SYSTEM AND INSPECTION METHOD FOR EARLY DIAGNOSIS OF PET PERIODONTAL DISEASE

Abstract

According to some embodiments of the present invention, an oral microbiome inspection system for early diagnosis of pet periodontal disease comprises: a genome analysis device configured to generate microbiome data on the basis of a specimen collected from the oral cavity of a pet to be inspected; and a processing device configured to generate abundance data, occurrence frequency data, and correlation data of the microbiome data on the basis of a list of microorganisms of interest in a pet standard database, generate health state data of the specimen by comparing the abundance data, the occurrence frequency data, and the correlation data with standard samples of the pet standard database, and generate an oral health report for the pet to be inspected, on the basis of the health state data.

Inventors

• Lee, Junhyung
• KIM, Seonkyun

Assignees

• 주식회사 리비오젠

Dates

Publication Date

20260507

Application Date

20250903

Priority Date

20241031

Claims (10)

1. In an oral microbiome testing system for the early diagnosis of periodontal disease in companion animals, A genomic analysis device configured to generate microbiome data based on a sample collected from the oral cavity of a pet subject to examination; and Generate abundance data, prevalence data, and correlation data of the above microbiome data based on the list of microorganisms of interest in the pet standard database, and Generate health status data of the specimen by comparing the above abundance data, the above frequency of occurrence data, and the above correlation data with standard samples of the above pet standard database, and A processing device configured to generate an oral health report for the pet subject to examination based on the above health status data; comprising The standard samples of the above pet standard database are, Primary standard samples including multiple disease-specific abundance data, incidence data, and correlation data including bad breath stage, plaque stage, tartar stage, gingivitis stage, and periodontal disease stage, and For each of the above primary standard samples, the disease progression stage is subdivided and constructed with additional secondary standard samples including abundance data, incidence data, and correlation data by disease progression level, and An oral microbiome testing system for the early diagnosis of periodontal disease in companion animals, characterized in that the above occurrence frequency data is provided as a heatmap that visually displays the frequency of appearance of each microorganism in two dimensions by indicating the probability that a specific microorganism in the specimens of the above primary standard samples and the above secondary standard samples exceeds a preset threshold in different colors.
2. In paragraph 1, An oral microbiome testing system for the early diagnosis of periodontal disease in companion animals, wherein the above-described genome analysis device is configured to generate microbiome data by performing 16S rRNA sequencing based on next-generation sequencing (NGS) to identify the types of microorganisms present in the sample.
3. In paragraph 1, The above list of microorganisms of interest specifies n phylums and m genera referenced for the early diagnosis of periodontal disease, and An oral microbiome testing system for the early diagnosis of periodontal disease in companion animals, configured such that the processing device generates the abundance data, the frequency data, and the correlation data based on the n phyla and the m genera.
4. In paragraph 3, The processing device generates the abundance data by calculating the first composition ratio of the n phyla and the second composition ratio of the m genera of microorganisms present in the sample. An oral microbiome testing system for early diagnosis of periodontal disease in pets, configured to generate health status data of the specimen by comparing the first composition ratio and the second composition ratio with the standard samples.
5. In paragraph 3, The processing device generates the occurrence frequency data by generating a first heatmap representing the probabilities that each of the n doors exceeds detection thresholds of relative abundance and a second heatmap representing the probabilities that each of the m attributes exceeds detection thresholds of relative abundance. An oral microbiome testing system for early diagnosis of periodontal disease in pets, configured to generate health status data of the specimen by comparing the first heatmap and the second heatmap with the standard samples.
6. In paragraph 3, The processing device generates correlation data by calculating m correlation values between m microbial communities present in the sample and a representative microbial community corresponding to the m genera, and An oral microbiome testing system for the early diagnosis of periodontal disease in pets, configured to generate health status data of the specimen by comparing the m correlation values with the standard samples.
7. In paragraph 1, The processing device determines the standard sample closest to the abundance data, the frequency data, and the correlation data for the sample using a data classification model trained to output the standard sample closest to the input data among the standard samples, and An oral microbiome testing system for the early diagnosis of periodontal disease in pets, configured to generate health status data based on a standard sample determined to be closest to the above specimen.
8. In Paragraph 7, The above processing device is configured to generate the oral health report by considering metadata including the species, age, gender, weight, oral care status, and specific diseases of the pet subject to examination, in an oral microbiome testing system for early diagnosis of periodontal disease in pets.
9. In paragraph 1, The processing device determines an evaluation indicator corresponding to the microbiome data based on the PCoA cluster of the pet standard database, and An oral microbiome testing system for the early diagnosis of periodontal disease in companion animals, configured to generate the oral health report by additionally considering the above evaluation indicators.
10. In a method for testing the oral microbiome for the early diagnosis of periodontal disease in companion animals, A step of generating microbiome data based on a sample collected from the oral cavity of a pet subject to examination using a genomic analysis device; A step of generating abundance data, occurrence frequency data, and correlation data of the microbiome data based on the list of microorganisms of interest in the pet standard database through a processing device; A step of generating health status data of the specimen by comparing the abundance data, the frequency of occurrence data, and the correlation data through the processing device with standard samples of the pet standard database; and The method includes the step of generating an oral health report for the pet subject to examination based on the health status data through the processing device. The standard samples of the above pet standard database are, Primary standard samples including multiple disease-specific abundance data, incidence data, and correlation data including bad breath stage, plaque stage, tartar stage, gingivitis stage, and periodontal disease stage, and For each of the above primary standard samples, the disease progression stage is subdivided and constructed with additional secondary standard samples including abundance data, incidence data, and correlation data by disease progression level, and A method for testing the oral microbiome for the early diagnosis of periodontal disease in companion animals, characterized in that the above occurrence frequency data is provided as a heatmap that visually displays the frequency of appearance of each microorganism in two dimensions by indicating the probability that a specific microorganism in the specimens of the above primary standard samples and the above secondary standard samples exceeds a preset threshold in different colors.

Description

Oral microbiome testing system and testing method for early diagnosis of periodontal disease in companion animals The present invention relates to a system and method for analyzing the oral microbiome of a pet to diagnose oral health conditions, such as periodontal disease, at an early stage. Previously, the oral health of pets was primarily assessed through visual inspection, dental X-rays, and direct oral examinations by veterinarians, with the status determined by external signs such as tooth condition, gum inflammation, and bad breath. Mechanical methods, such as tartar removal, were the main approach to managing oral biofilm; veterinarians physically removed tartar using dental instruments or reduced biofilm through tooth rinsing. While some methods were used to inhibit the growth of oral bacteria using antimicrobial agents or mouthwashes, these were limited to superficial bacterial removal, making it difficult to regulate the balance of the microbial community. Existing technologies had problems and room for improvement in various aspects. While visual inspection and physical methods are effective for detecting advanced diseases, they had limitations in detecting changes in the microbial community or subtle imbalances in the early stages of disease. Existing microbial analysis techniques were useful for confirming the presence of microorganisms, but it was difficult to apply them directly to predicting health status or preventive management. Mechanical removal methods for managing biofilms were inefficient because they caused pain and stress during tartar removal, posed risks due to the need for general anesthesia, and were costly and cumbersome as they required regular procedures. The use of antimicrobial agents or detergents was effective for short-term bacterial reduction, but it was difficult to regulate the long-term balance of the oral microbial community or protect beneficial bacteria through them. Research on the oral microbiome of companion animals is relatively limited compared to humans, and there has been a lack of predictive models that reflect species-specific differences. Existing technologies have made it difficult to precisely assess the oral health status of individual companion animals. In particular, when applying oral microbiome data to health prediction and personalized preventive care, there has been a lack of standardized methods for clinically interpreting analysis results. (Prior Art Literature) Patent documents Patent Document 1: Published Patent Application No. 10-2023-0173676 (December 27, 2023) Patent Document 2: Published Patent Application No. 10-2024-0033007 (March 12, 2024) FIG. 1 illustrates an environment in which an oral microbiome testing system for early diagnosis of periodontal disease in companion animals, according to some embodiments, operates. FIG. 2 illustrates elements constituting an oral microbiome testing system for the early diagnosis of periodontal disease in companion animals according to some embodiments. FIG. 3 illustrates the process of an oral microbiome testing system according to some embodiments generating an oral health report based on an oral sample. FIG. 4 illustrates a method of collecting a sample from the oral cavity of a pet subject to examination according to some embodiments. FIG. 5 illustrates a method for designing and optimizing primers and probes for biomarkers according to some embodiments. FIG. 6 illustrates abundance data according to some embodiments. FIG. 7 illustrates prevalence data according to some embodiments. FIGS. 8 and 9 illustrate correlation data according to some embodiments. FIG. 10 illustrates a method for classifying the condition of a sample based on a PCoA cluster regarding a representative microorganism according to some embodiments. FIG. 11 illustrates metadata additionally considered to generate health status data of a pet subject to examination according to some embodiments. FIG. 12 illustrates steps constituting an oral microbiome test method for early diagnosis of periodontal disease in companion animals according to some embodiments. An oral microbiome testing system for the early diagnosis of periodontal disease in companion animals comprises: a genomic analysis device configured to generate microbiome data based on a sample collected from the oral cavity of a companion animal to be tested; A processing device configured to generate abundance data, prevalence data, and correlation data of the microbiome data based on a list of microorganisms of interest in a pet standard database, generate health status data of the specimen by comparing the abundance data, prevalence data, and correlation data with standard samples of the pet standard database, and generate an oral health report for the pet subject to examination based on the health status data; wherein the standard samples of the pet standard database are constructed of primary standard samples including abundance data, prevalence data, and correlatio